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Citas y referencias (48)
Invitrogen™
MANT-GTP (2'-(or-3')-O-(N-Methylanthraniloyl) Guanosine 5'-Triphosphate, Trisodium Salt)
El análogo nucleótido MANT GTP se modifica en la fracción de la ribosa. La madurez compacta del fluoróforo de MANTMás información
| Número de catálogo | Cantidad |
|---|---|
| M12415 | 400 μl |
Número de catálogo M12415
Precio (CLP)
484.526
Each
Cantidad:
400 μl
Precio (CLP)
484.526
Each
El análogo nucleótido MANT GTP se modifica en la fracción de la ribosa. La madurez compacta del fluoróforo de MANT y su posición de fijación resulta en análogos de nucleótidos que inducen una perturbación mínima de las interacciones de nucleótidos-proteínas. Debido a que la fluorescencia de MANT es sensible al ambiente del fluoróforo, las interacciones nucleótido-proteína pueden ser directamente detectables. Los nucleótidos MANT son valiosas sondas de la estructura y actividad enzimática de las proteínas de unión a nucleótidos.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Etiqueta o tinteMANT (N-Methylanthraniloyl)
Tipo de productoMANT-GTP
Cantidad400 μl
Condiciones de envíoHielo húmedo
Concentración5 mM
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.
Citations & References (48)
Citations & References
Abstract
Journal:
PubMed ID:11063593
Activation of the superoxide-generating NADPH oxidase by chimeric proteins consisting of segments of the cytosolic component p67(phox) and the small GTPase Rac1.
Journal:Biochemistry
PubMed ID:11724569
'Activation of the superoxide (O2(-))-generating NADPH oxidase of phagocytes is the consequence of the assembly of a membrane-associated flavocytochrome b(559) with the cytosolic proteins p47(phox) and p67(phox) and the small GTPase Rac (1 or 2). We proposed that Rac1 serves as a membrane-targeting molecule for p67(phox). This hypothesis was tested
A novel function for Cyclin A2: control of cell invasion via RhoA signaling.
Journal:J Cell Biol
PubMed ID:22232705
'Cyclin A2 plays a key role in cell cycle regulation. It is essential in embryonic cells and in the hematopoietic lineage yet dispensable in fibroblasts. In this paper, we demonstrate that Cyclin A2-depleted cells display a cortical distribution of actin filaments and increased migration. These defects are rescued by restoration
Low affinity interactions of GDPbetaS and ribose- or phosphoryl-substituted GTP analogues with the heterotrimeric G protein, transducin.
Journal:J Biol Chem
PubMed ID:8662741
'We have examined the effects of three commonly used classes of guanine nucleotide analogues on the retinal G protein, transducin (Gt), and found them to be quite different from those that might be expected from results with other GTP-binding proteins. The most surprising results were with guanosine 5''-O-(2-thiodiphosphate) (GDPbetaS); rather
Alanine scan mutagenesis of the switch I domain of the Caulobacter crescentus CgtA protein reveals critical amino acids required for in vivo function.
Journal:Mol Microbiol
PubMed ID:11251813
'The Caulobacter crescentus CgtA protein is a member of the Obg/GTP1 subfamily of monomeric GTP-binding proteins. In vitro, CgtA displays moderate affinity for both GDP and GTP and displays rapid exchange rate constants for either nucleotide, indicating that the guanine nucleotide-binding and exchange properties of CgtA are different from those