NeuroTrace™ 530/615 Red Fluorescent Nissl Stain - Solution in DMSO
NeuroTrace™ 530/615 Red Fluorescent Nissl Stain - Solution in DMSO
Citas y referencias (21)
Invitrogen™

NeuroTrace™ 530/615 Red Fluorescent Nissl Stain - Solution in DMSO

La tinción de Nissl es el método histológico estándar para visualizar neuronas en cerebro y médula espinal Compuesto de ARNMás información
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Número de catálogoCantidad
N214821 mL
Número de catálogo N21482
Precio (CLP)
316.146
Each
Añadir al carro de la compra
Cantidad:
1 mL
Precio (CLP)
316.146
Each
Añadir al carro de la compra
La tinción de Nissl es el método histológico estándar para visualizar neuronas en cerebro y médula espinal Compuesto de ARN ribosomal asociado con el retículo endoplasmático áspero en pericaria neuronal y dendritas, la sustancia Nissl se redistribuye dentro del cuerpo celular en neuronas lesionadas o en regeneración, proporcionando un marcador del estado fisiológico de la neurona Nuestra tinción Nissl fluorescente rojo NeuroTrace 530/615 es un selectivo para la sustancia Nissl característica de las neuronas y proporciona más sensibilidad que los colorantes histológicos tradicionales, como el azul de toluidina o el violeta de cresilo.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
ColorNaranja
Método de detecciónFluorescencia
Para utilizar con (equipo)Microscopio de fluorescencia
Tipo de productoTinción de Nissl
Cantidad1 mL
Condiciones de envíoTemperatura ambiente
Localización subcelularCuerpos de Nissl
Excitation/Emission530/615 nm
Línea de productosNEUROTRACE
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador (de -5 a -30 °C) y proteger de la luz.

Preguntas frecuentes

I am labeling brain cryosections with a NeuroTrace Nissl Stain. Is this compatible with antibody labeling?

Yes. We have done this successfully with an anti-GFAP primary and an Alexa Fluor secondary antibody. We would recommend labeling with the primary and secondary antibodies first, then following up with the standard NeuroTrace Nissl Stain protocol.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I can use the NeuroTrace Nissl stains for staining glia or other cell types. What can I do to improve the staining so that it is more selective for neurons?

Our NeuroTrace Nissl stains label the Nissl substance which is composed of ribosomal RNA associated with the rough endoplasmic reticulum and is present in high amounts in neuronal cells. These dyes are not completely specific for neurons, but will selectively stain neurons based on their high level of protein synthesis. In some cases they may show staining of other cell types such as glia, so you may need to decrease the staining concentration to obtain more selective neuronal labeling. We suggest dilutions in the range of 20- to 300-fold for neuronal staining.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can the NeuroTrace Nissl stains be used on paraffin sections?

We have only tested them on mouse brain cryosections, however, there is at least one citation describing their use on paraffin tissue sections (Michelle L. Schlief, Ann Marie Craig, and Jonathan D. Gitlin. NMDA Receptor Activation Mediates Copper Homeostasis in Hippocampal Neurons. The Journal of Neuroscience, January 5, 2005, 25(1):239 - 246).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What are the NeuroTrace Nissl stains?

The dyes are proprietary, however they are stains that label the Nissl substance, which is composed of ribosomal RNA associated with the rough endoplasmic reticulum and is present in high amounts in neuronal cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What products do you have for neuronal tracing?

Please check out this web page (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing.html) for details.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (21)

Citations & References
Abstract
Transgenic labeling of the corticospinal tract for monitoring axonal responses to spinal cord injury.
Authors:Bareyre FM, Kerschensteiner M, Misgeld T, Sanes JR
Journal:Nat Med
PubMed ID:16286922
'The rodent corticospinal tract (CST) has been used extensively to investigate regeneration and remodeling of central axons after injury. CST axons are currently visualized after injection of tracer dye, which is invasive, incomplete and prone to variation, and often does not show functionally crucial but numerically minor tract components. Here, ... More
A new method for imaging and 3D reconstruction of mammalian cochlea by fluorescent confocal microscopy.
Authors:Hardie NA, MacDonald G, Rubel EW
Journal:Brain Res
PubMed ID:15053969
'Traditional methods for anatomical and morphometric studies of cochlear tissues have relied upon either microdissection of the organ of Corti or the generation of serial sections of the cochlea. Such methods are time-consuming, disruptive to three-dimensional relationships and often restrict sampling to very limited numbers of cells. We have found ... More
Characterization of nestin expression in the spinal cord of GFP transgenic mice after peripheral nerve injury.
Authors:Matsumura S, Takagi K, Okuda-Ashitaka E, Lu J, Naritsuka H, Yamaguchi M, Ito S,
Journal:Neuroscience
PubMed ID:20673789
'Many studies have shown that activation and increase in the number of astrocytes and microglia in the spinal cord participate in the initiation and maintenance of neuropathic pain, but little attention has been paid to the responses of neural progenitor cells to peripheral nerve injury. Nestin, a class VI intermediate ... More
Synfire chains and cortical songs: temporal modules of cortical activity.
Authors:Ikegaya Y, Aaron G, Cossart R, Aronov D, Lampl I, Ferster D, Yuste R
Journal:Science
PubMed ID:15105494
How can neural activity propagate through cortical networks built with weak, stochastic synapses? We find precise repetitions of spontaneous patterns of synaptic inputs in neocortical neurons in vivo and in vitro. These patterns repeat after minutes, maintaining millisecond accuracy. Calcium imaging of slices reveals reactivation of sequences of cells during ... More
RNAi reveals doublecortin is required for radial migration in rat neocortex.
Authors:Bai J, Ramos RL, Ackman JB, Thomas AM, Lee RV, LoTurco JJ
Journal:Nat Neurosci
PubMed ID:14625554
Mutations in the doublecortin gene (DCX) in humans cause malformation of the cerebral neocortex. Paradoxically, genetic deletion of Dcx in mice does not cause neocortical malformation. We used electroporation of plasmids encoding short hairpin RNA to create interference (RNAi) of DCX protein in utero, and we show that DCX is ... More
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