Kit de cuantificación de proteínas NanoOrange™, 200-2000 ensayos

Citas y referencias (29)

Invitrogen™

Kit de cuantificación de proteínas NanoOrange™, 200-2000 ensayos

El kit de cuantificación de proteínas NanoOrange contiene en ensayo muy sensible y sencillo para la cuantificación de proteínas, conMás información

Número de catálogo	Cantidad
N6666	1 kit

Número de catálogo N6666

Precio (CLP)

510.872

Añadir al carro de la compra

Cantidad:

1 kit

Pedido a granel o personalizado

Precio (CLP)

510.872

Añadir al carro de la compra

El kit de cuantificación de proteínas NanoOrange contiene en ensayo muy sensible y sencillo para la cuantificación de proteínas, con detección de concentraciones de tan solo 10 ng/ml de proteína en solución. Este tinte fluorescente es adecuado para el uso con espectrofluorímetros y lectores de microplacas. Para la detección de lipoproteínas o proteínas en un entorno complejo de lípidos, consulte nuestro kit de cuantificación de proteínas CBQCA (C-6667).

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

ensayoEnsayo de proteínas fluorescentes

Para utilizar con (aplicación)Cuantificación de proteínas

Para utilizar con (equipo)Lector de microplacas

Línea de productosNanoOrange

Tipo de productoEnsayo de cuantificación de proteínas

Cantidad1 kit

Suficiente paraDe 200 a 2000 ensayos

Método de detecciónFluorescencia

Unit SizeEach

Preguntas frecuentes

When preparing the serial dilutions for the standard curve for the NanoOrange Protein Quantitation Kit (Cat. No. N6666), can they first be diluted in buffer (e.g., Tris) or do they have to be diluted in 1X NanoOrange working solution?

All samples and standards need to be diluted in 1X NanoOrange working solution, not in any other buffer.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

My buffer or components of my buffer are not listed in the compatibility table for my protein assay. What should I do?

You can test the tolerance of the assay for your specific buffer formulation. For in-house generated compatibility information, substances were considered compatible at the indicated concentration in the Standard Test Tube Protocol (found in the manual for each protein assay) if the error in protein concentration estimation caused by the presence of the substance was less than or equal to 10%. The substances were tested using WR prepared immediately before each experiment. Blank-corrected 562nm absorbance measurements (for a 1000µg/mL BSA standard + substance) were compared to the net 562nm measurements of the same standard prepared in 0.9% saline.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

All the components of my sample buffer are at or below the indicated compatible concentration for my protein assay, but I am still seeing too much/too little color development. What could be the problem?

It is possible to have a substance additive affect such that even though a single component is present at a concentration below its listed compatibility, a sample buffer containing a combination of substances could interfere with the assay. You should take steps to eliminate or minimize the effects of the interfering substance(s) by diluting or removing the substance.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

My protein assay is not developing color or is developing too much color. What can I do?

Refer to the information in the product-specific instruction booklet or our Tech Tip: Protein Quantitation Assay Compatibility Table (https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/TR0068-Protein-assay-compatibility.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

My spectrophotometer doesn’t have a filter set for the absorbance maximum. Can I use an alternate wavelength to read the protein assay?

Often, an alternative wavelength can be used, although the slope of the standard curve and the overall assay sensitivity will most likely be reduced. Our Tech Tip (https://tools.thermofisher.com/content/sfs/brochures/TR0025-Protein-assay-spectra.pdf) offers additional information on determining acceptable wavelengths for measuring protein assays.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all Kit de cuantificación de proteínas NanoOrange™, 200-2000 ensayos FAQs

Citations & References (29)

Citations & References

Abstract

Authors:

Journal:

PubMed ID:10652312

Identification of cyclic AMP-regulated genes in Mycobacterium tuberculosis complex bacteria under low-oxygen conditions.

Authors:Gazdik MA, McDonough KA

Journal:J Bacteriol

PubMed ID:15805514

'Mycobacterium tuberculosis is the etiological agent of tuberculosis (TB), which kills approximately 2 million people a year despite current treatment options. A greater understanding of the biology of this bacterium is needed to better combat TB disease. The M. tuberculosis genome encodes as many as 15 adenylate cyclases, suggesting that ... More

Regulation of collagen deposition and lysyl oxidase by tumor necrosis factor-alpha in osteoblasts.

Authors:Pischon N, Darbois LM, Palamakumbura AH, Kessler E, Trackman PC

Journal:J Biol Chem

PubMed ID:15138266

'Tumor necrosis factor-alpha (TNF-alpha) inhibits osteoblast function in vitro by inhibiting collagen deposition. Studies generally support that TNF-alpha does not inhibit collagen biosynthesis by osteoblasts but that collagen deposition is in some way diminished. The study investigated TNF-alpha regulation of biosynthetic enzymes and proteins crucial for posttranslational extracellular collagen maturation ... More

Control of neuronal size homeostasis by trophic factor-mediated coupling of protein degradation to protein synthesis.

Authors:Franklin JL, Johnson EM

Journal:J Cell Biol

PubMed ID:9732291

'We demonstrate that NGF couples the rate of degradation of long-lived proteins in sympathetic neurons to the rate of protein synthesis. Inhibiting protein synthesis rate by a specific percentage caused an almost equivalent percentage reduction in the degradation rate of long-lived proteins, indicating nearly 1:1 coupling between the two processes. ... More

Key role of conserved histidines in recombinant mouse beta-carotene 15,15'-monooxygenase-1 activity.

Authors:Poliakov E, Gentleman S, Cunningham FX, Miller-Ihli NJ, Redmond TM

Journal:J Biol Chem

PubMed ID:15951442

'Alignment of sequences of vertebrate beta-carotene 15,15''-monooxygenase-1 (BCMO1) and related oxygenases revealed four perfectly conserved histidines and five acidic residues (His172, His237, His308, His514, Asp52, Glu140, Glu314, Glu405, and Glu457 in mouse BCMO1). Because BCMO1 activity is iron-dependent, we propose that these residues participate in iron coordination and therefore are ... More

View all Kit de cuantificación de proteínas NanoOrange™, 200-2000 ensayos citations