PFB-FDGlu (5-(Pentafluorobenzoylamino)Fluorescein Di-β-D-Glucopyranoside)

Citas y referencias (16)

Invitrogen™

PFB-FDGlu (5-(Pentafluorobenzoylamino)Fluorescein Di-β-D-Glucopyranoside)

El sustrato ß-glucosidasa, 5-(pentafluorobenzoilo)fluoresceína di-ß-D-glucopiranósido (PFB-FDGlu) produce el colorante verde-fluorescente PFB-F (excitación/emisión máxima aprox. ∼492/516 nm), que parece localizar losMás información

Número de catálogo	Cantidad
P11947 también denominado P-11947	5 mg

Número de catálogo P11947

también denominado P-11947

Precio (CLP)

Cantidad:

El sustrato ß-glucosidasa,

5-(pentafluorobenzoilo)fluoresceína di-ß-D-glucopiranósido (PFB-FDGlu)
produce el colorante verde-fluorescente PFB-F (excitación/emisión máxima aprox. ∼492/516 nm), que parece localizar los compartimentos endosómico y lisosómico cuando se carga en células por pinocitosis.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Permeabilidad celularPenetra en la célula

ColorGreen

Excitación/emisión492⁄516

Para utilizar con (equipo)Citómetro de flujo

Etiqueta o tinteFluorescein

Tipo de productoSustrato

Cantidad5 mg

Condiciones de envíoTemperatura ambiente

Requisitos de almacenamientoFreezer (-5°C to -30°C)

SustratoSustrato beta-glucosidasa

Método de detecciónFluorescente

FormularioPolvo

Substrate PropertiesSustrato químico

Tipo de sustratoBeta-Glucosidase Substrate

Target EnzymeBeta-Glucosidase

Unit SizeEach

Contenido y almacenamiento

Almacenar en congelador (de -5 a -30 °C).

Citations & References (16)

Citations & References

Abstract

Activation and Purification of ß-Glucocerebrosidase by Exploiting its Transporter LIMP-2 - Implications for Novel Treatment Strategies in Gaucher's and Parkinson's Disease.

Authors:Dobert JP,Bub S,Mächtel R,Januliene D,Steger L,Regensburger M,Wilfling S,Chen JX,Dejung M,Plötz S,Hehr U,Moeller A,Arnold P,Zunke F

Journal:Advanced science (Weinheim, Baden-Wurttemberg, Germany)

PubMed ID:38666485

Genetic variants of GBA1 can cause the lysosomal storage disorder Gaucher disease and are among the highest genetic risk factors for Parkinson's disease (PD). GBA1 encodes the lysosomal enzyme beta-glucocerebrosidase (GCase), which orchestrates the degradation of glucosylceramide (GluCer) in the lysosome. Recent studies have shown that GluCer accelerates α-synuclein aggregation, ... More

Late-onset Krabbe disease presenting as spastic paraplegia - implications of GCase and CTSB/D.

Authors:Mächtel R,Dobert JP,Hehr U,Weiss A,Kettwig M,Laugwitz L,Groeschel S,Schmidt M,Arnold P,Regensburger M,Zunke F

Journal:Annals of clinical and translational neurology

PubMed ID:38837642

Krabbe disease (KD) is a multisystem neurodegenerative disorder with severe disability and premature death, mostly with an infancy/childhood onset. In rare cases of late-onset phenotypes, symptoms are often milder and difficult to diagnose. We here present a translational approach combining diagnostic and biochemical analyses of a male patient with a ... More

The Parkinson's disease risk gene cathepsin B promotes fibrillar alpha-synuclein clearance, lysosomal function and glucocerebrosidase activity in dopaminergic neurons.

Authors:Jones-Tabah J,He K,Karpilovsky N,Senkevich K,Deyab G,Pietrantonio I,Goiran T,Cousineau Y,Nikanorova D,Goldsmith T,Del Cid Pellitero E,Chen CX,Luo W,You Z,Abdian N,Ahmad J,Ruskey JA,Asayesh F,Spiegelman D,Fahn S,Waters C,Monchi O,Dauvilliers Y,Dupré N,Miliukhina I,Timofeeva A,Emelyanov A,Pchelina S,Greenbaum L,Hassin-Baer S,Alcalay RN,Milnerwood A,Durcan TM,Gan-Or Z,Fon EA

Journal:Molecular neurodegeneration

PubMed ID:39587654

Variants in the CTSB gene encoding the lysosomal hydrolase cathepsin B (catB) are associated with increased risk of Parkinson's disease (PD). However, neither the specific CTSB variants driving these associations nor the functional pathways that link catB to PD pathogenesis have been characterized. CatB activity contributes to lysosomal protein degradation ... More

Flow Cytometry Measurement of Glucocerebrosidase Activity in Human Monocytes.

Authors:Hughes LP,Halliday GM,Dzamko N

Journal:Bio-protocol

PubMed ID:33659542

Glucocerebrosidase (GCase) is an important enzyme for the metabolism of glycolipids. GCase enzyme deficiency is implicated in human disease and the efficient measurement of GCase activity is important for evaluating the efficacy of therapeutics targeting this enzyme. Existing approaches to measure GCase activity include whole blood mass spectrometry-based assays, where ... More

Tau accumulation in degradative organelles is associated to lysosomal stress.

Authors:Piovesana E,Magrin C,Ciccaldo M,Sola M,Bellotto M,Molinari M,Papin S,Paganetti P

Journal:Scientific reports

PubMed ID:37865674

Neurodegenerative disorders are characterized by the brain deposition of insoluble amyloidogenic proteins, such as α-synuclein or Tau, and the concomitant deterioration of cell functions such as the autophagy-lysosomal pathway (ALP). The ALP is involved in the degradation of intracellular macromolecules including protein aggregates. ALP dysfunction due to inherited defects in ... More

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