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Citas y referencias (32)
Invitrogen™
1-pyrenebutanoic acid, succinimidyl ester
El ácido 1-pirenebutanoico reactivo a la amina, éster succinimidil puede usarse para crear bioconjugados sensibles al medio ambiente con esteMás información
| Número de catálogo | Cantidad |
|---|---|
| P130 | 100 mg |
Número de catálogo P130
Precio (CLP)
-
Cantidad:
100 mg
El ácido 1-pirenebutanoico reactivo a la amina, éster succinimidil puede usarse para crear bioconjugados sensibles al medio ambiente con este fluoróforo único.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Reactividad químicaAmina
Etiqueta o tintePireno
Tipo de productoÁcido 1-pirenebutanoico, éster succinimidilo
Cantidad100 mg
Fracción reactivaEster activo, succinimidilo éster
Condiciones de envíoTemperatura ambiente
Tipo de etiquetaColorantes clásicos
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador (de – 5 a – 30 °C) y proteger de la luz.
Citations & References (32)
Citations & References
Abstract
RNA tertiary folding monitored by fluorescence of covalently attached pyrene.
Journal:Biochemistry
PubMed ID:10571996
'The pathways by which large RNAs adopt tertiary structure are just beginning to be explored, and new methods that reveal RNA folding are highly desirable. Here we report an assay for RNA tertiary folding in which the fluorescence of a covalently incorporated chromophore is monitored. Folding of the 160-nucleotide Tetrahymena
Effects of Mg2+ and the 2' OH of guanosine on steps required for substrate binding and reactivity with the Tetrahymena ribozyme reveal several local folding transitions.
Journal:Biochemistry
PubMed ID:9287156
'Transient kinetic studies with fluorescence detection were used to determine the effects of Mg2+ concentration and the 2' OH group of guanosine monophosphate, prG, substrate on various steps in the transesterification reaction of prG with 5' pyrene-labeled oligonucleotides as catalyzed by the L-21 ScaI ribozyme. The effect of increasing Mg2+
Human plasma fibronectin structure probed by steady-state fluorescence polarization: evidence for a rigid oblate structure.
Journal:Biochemistry
PubMed ID:2337580
'In order to more clearly define the structure of human plasma fibronectin (PFn) under physiologic buffer conditions, we determined the mean harmonic rotational relaxation times (rho H) of PFn and the thrombin-derived 190/170-kDa PFn fragment using steady-state fluorescence polarization. These measurements utilized the long lifetime emission (tau = 1.2 X
Spontaneous and plasma factor-mediated transfer of pyrenyl cerebrosides between model and native lipoproteins.
Journal:Biochim Biophys Acta
PubMed ID:3931685
'A series of pyrenyl glucocerebrosides was synthesized by reacylation of psychosine with pyrene-labeled fatty acids having 3-11 methylene units. When incorporated into model high-density lipoproteins consisting of dimyristoylphosphatidylcholine-apolipoprotein A-II complexes and incubated with unlabeled complexes, these lipids exhibited spontaneous transfer. Half times of transfer varied from 1.5 min to 365
Productive and nonproductive intermediates in the folding of denatured rhodanese.
Journal:J Biol Chem
PubMed ID:10617586
'The competition between protein aggregation and folding has been investigated using rhodanese (thiosulfate:cyanide sulfurtransferase, EC 2.8.1.1) as a model. During folding from a urea-denatured state, rhodanese rapidly forms associated species or intermediates, some of which are large and/or sticky. The early removal of such particles by filtration results in a