FP One-Step Reference Kit
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FP One-Step Reference Kit

Muchos instrumentos de lectura de polarización de fluorescencia (FP) necesitan utilizar un estándar de referencia para la calibración interna delMás información
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Número de catálogoCantidad
P30881 kit
Número de catálogo P3088
Precio (CLP)
654.481
Each
Añadir al carro de la compra
Cantidad:
1 kit
Precio (CLP)
654.481
Each
Añadir al carro de la compra
Muchos instrumentos de lectura de polarización de fluorescencia (FP) necesitan utilizar un estándar de referencia para la calibración interna del lector. El kit de referencia FP One Step contiene reactivos para su uso como referencias FP verdes o rojas en un solo tubo o en un instrumento de detección de alto rendimiento (HTS) de microplacas. Los reactivos verdes se pueden utilizar para las lecturas utilizando los siguientes filtros: 488 nm ± 5 nm para excitación y 535 nm ± 5 nm para emisión (o equivalente). Los reactivos rojos se pueden utilizar para las lecturas utilizando los siguientes filtros: 525 nm ± 5 nm para excitación y 590 nm ± 5 nm para emisión (o equivalente).
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Para utilizar con (equipo)Beacon™ 2000, lector de microplacas
Cantidad1 kit
Almacenamiento recomendadoTodos los componentes del kit deben almacenarse a 20-30 °C.
Condiciones de envíoAprobado para su envío a temperatura ambiente o en hielo húmedo o seco
Unit SizeEach

Preguntas frecuentes

What are the common issues encountered with FP assays?

-No assay window: Check that your instrument is designed to do FP experiments.
-Poor discrimination between the minimum and maximum mP Control (both include the receptor): This may be an indication of the quality of the receptor. Do not make single use aliquots of the receptor or store diluted.
-The type of microplate is critical: Please see if the protocol specifies untreated polystyrene or NBS-coated plates. If the plate binds the fluorescent substrate, it cannot freely rotate and the mP reading will always be high. White plates cannot be used for FP assays. Avoid the use of silanized pipette tips and vials.
-The “No Receptor Control' (free Fluormone/Tracer) should have much higher RFU values compared to buffer alone. If not, there is a problem with the instrument setup or less commonly with the Fluormone/Tracer.
-If the free Fluormone/Tracer mP value is greater than 100 mP or negative, please refer to the user manual for your instrument and reset the G factor.

Find additional tips, troubleshooting help, and resources within our Drug Discovery & Development Support Center.

What is the best type of microplate reader for fluorescence polarization (FP) assays?

For FP assays, plate readers must specifically have FP built in. Please see our instrument compatibility portal (http://www.thermofisher.com/us/en/home/industrial/pharma-biopharma/drug-discovery-development/dd-misc/instrument-compatibility-portal.html).

Find additional tips, troubleshooting help, and resources within our Drug Discovery & Development Support Center.

How can I test the fluorescence polarization (FP) settings of my microplate reader before doing the assay?

We offer a FP One-Step Reference Kit (https://www.thermofisher.com/order/catalog/product/P3088) to detect in green (Ex/Em = 488/535 nm) and red (Ex/Em = 525/590 nm) emission ranges, suitable for the PolarScreen Green and Red Assays. The alternative is to perform the Controls in the respective assay kit prior to performing the actual assay using test compounds.

Find additional tips, troubleshooting help, and resources within our Drug Discovery & Development Support Center.