Fluorescein-Ubiquitin
Fluorescein-Ubiquitin
Citas y referencias (1)

Fluorescein-Ubiquitin

Fluorescein-labeled ubiquitin for use as a general research tool or in high throughput assays to monitor protein ubiquitination. Due toMás información
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Número de catálogoCantidad
PV4378500 μg
PV437750 μg
Número de catálogo PV4378
Precio (CLP)
1.891.809
Each
Añadir al carro de la compra
Cantidad:
500 μg
Precio (CLP)
1.891.809
Each
Añadir al carro de la compra
Fluorescein-labeled ubiquitin for use as a general research tool or in high throughput assays to monitor protein ubiquitination. Due to the method in labeling the ubiquitin protein, all of the lysines with the protein are available for further conjugation to form poly-ubiquitin chains.

For use in combination with FRET donors including LanthaScreen™ Tb-Ubiquitin (PV4375) or a LanthaScreen™ antibody including Tb-anti-GST (PV4216) or Tb-anti-ubiquitin-FK2 (PV4751).
For Research Use Only. Not for use in diagnostic procedures.
Especificaciones
Método de detecciónFluorescence
Excitación/emisión340⁄490, 520
Para utilizar con (equipo)Microplate Reader
Tipo de etiquetaClassic Dyes
Etiqueta o tinteFITC (Fluorescein)
Cantidad500 μg
Presentación de datosEnd Point
Condiciones de envíoDry Ice
Propiedades de sustratoProtein-Based Substrate
TécnicaTR-FRET
ConjugadoFluorescein
FormularioLiquid
Unit SizeEach
Contenido y almacenamiento
Fluorescein-labeled ubiquitin in HEPES buffered saline. Store at -20°C.

Citations & References (1)

Citations & References
Abstract
TR-FRET-based high-throughput screening assay for identification of UBC13 inhibitors.
Authors:Madiraju C, Welsh K, Cuddy MP, Godoi PH, Pass I, Ngo T, Vasile S, Sergienko EA, Diaz P, Matsuzawa S, Reed JC,
Journal:J Biomol Screen
PubMed ID:22034497
UBC13 is a noncanonical ubiquitin conjugating enzyme (E2) that has been implicated in a variety of cellular signaling processes due to its ability to catalyze formation of lysine 63-linked polyubiquitin chains on various substrates. In particular, UBC13 is required for signaling by a variety of receptors important in immune regulation, ... More