Qtracker™ 565 Vascular Labels
Qtracker™ 565 Vascular Labels
Citas y referencias (9)
Invitrogen™

Qtracker™ 565 Vascular Labels

Los puntos cuánticos no dirigidos Qtracker™ están diseñados para ser inyectados en la vena de la cola de ratones paraMás información
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Número de catálogoCantidad
Q21031MP200 μl
Número de catálogo Q21031MP
Precio (CLP)
856.799
Each
Añadir al carro de la compra
Cantidad:
200 μl
Precio (CLP)
856.799
Each
Añadir al carro de la compra
Los puntos cuánticos no dirigidos Qtracker™ están diseñados para ser inyectados en la vena de la cola de ratones para el estudio de la estructura vascular usando técnicas de obtención de imágenes de animales pequeños in vivo (SAIVI). Estos nanocristales muestran una intensa fluorescencia con emisión de desplazamiento rojo para aumentar la penetración de los tejidos, y tienen un revestimiento de superficie de PEG especialmente desarrollado para minimizar las interacciones inespecíficas y reducir la respuesta inmune del tejido. Debido a que el revestimiento de la superficie de PEG no contiene grupos funcionales reactivos, los puntos cuánticos no dirigidos Qtracker™ se conservan en circulación durante más tiempo y se pueden exponer durante hasta 3 horas con una sola inyección o durante periodos de tiempo más largos con inyecciones adicionales.

¿Necesita otro espectro de emisión o un seguimiento más prolongado? Consulte nuestros otros productos de seguimiento de células de mamífero.

Atributos principales:

• La etiqueta Qtracker™ 565 tiene Ex/Em (405-525/655) nm
• Diseñados para la adquisición de imágenes in vivo de animales pequeños
• Se introducen mediante inyección en la vena de la cola, se puede adquirir imágenes hasta 3 horas después de la inyección
• Disponibles en cuatro colores: emisión de 565 nm, 655 nm, 705 nm u 800 nm

Obtenga más información sobre SAIVI y sobre las aplicaciones de los nanocristales Qdot™.

Para uso exclusivo en investigación. No diseñado para uso terapéutico o de diagnóstico en animales o humanos.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Concentración2 μM
Tipo de coloranteNanocristales Qdot
Línea de productosQTRACKER, Qdot
Cantidad200 μl
Tipo de reactivoReactivos para imágenes vasculares
Condiciones de envíoTemperatura ambiente
TécnicaObtención de imágenes in vivo
Tipo de productoEtiqueta
Unit SizeEach
Contenido y almacenamiento
Contiene 200 μl de puntos cuánticos no dirigidos Qtracker™ (solución de 2 μM en 50 mM de tampón de borato, pH de 8,3). Almacenar de 2–6 °C. No la congele. Estable durante al menos 6 meses.

Preguntas frecuentes

When I label with Qtracker cell labeling reagents, I get a punctate label pattern. How do I make it more uniform?

Qtracker cell labeling reagents are taken up by the cell through endocytosis and sequestered in endosomes. This gives the label a punctate or vesicular appearance. This is normal. There is nothing that can be done to make it appear uniform throughout the cytoplasm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How long after injection should I start imaging my mouse?

The imaging time course varies with the nature of the injected agent. Vascular tracers are visible in the blood vessels immediately after injection and may be imaged for several hours. Conjugated whole IgG antibodies reach their targets within a few hours of injection and may be imaged for several days.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What happens to unconjugated dye in the mouse after small animal in vivo imaging?

The dye will be eliminated via the bladder. The bladder signal is detectable within ~3 minutes of IV injection of the dye and clearance with ~30 minutes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What amount of Qtracker non-targeted reagent should I inject to image vasculature?

A recommended starting dosage is 25-50 µL of Qtracker reagent diluted to the desired injection volume with PBS or normal saline. Qtracker reagent should be diluted immediately prior to injection. DO NOT STORE DILUTED. You will need to determine the optimal dosage for your experimental models.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to track my cells with a nucleic acid stain, like DAPI or Hoechst dye. Do you recommend this?

This is not recommended. When these stains bind to DNA and RNA, they may affect the normal function of the nucleic acids, disrupting transcription, as well as replication. Other reagents, such as CellTracker dyes or Qtracker reagents are more optimized for tracking without disrupting normal activity. If a nuclear label is still desired, though, and the cells are mammalian and non-hematopoietic, CellLight nuclear reagents can transiently transfect cells to express GFP or RFP on a nuclear-expressing protein for up to several days without affecting function.

Find additional tips, troubleshooting help, and resources within our Cell Tracing and Tracking Support Center.

View all Qtracker™ 565 Vascular Labels FAQs

Citations & References (9)

Citations & References
Abstract
Intracellular fluid flow in rapidly moving cells.
Authors:Keren K, Yam PT, Kinkhabwala A, Mogilner A, Theriot JA,
Journal:Nat Cell Biol
PubMed ID:19767741
'Cytosolic fluid dynamics have been implicated in cell motility because of the hydrodynamic forces they induce and because of their influence on transport of components of the actin machinery to the leading edge. To investigate the existence and the direction of fluid flow in rapidly moving cells, we introduced inert ... More
A novel method to allow noninvasive, longitudinal imaging of the murine immune system in vivo.
Authors:Gibson VB, Benson RA, Bryson KJ, McInnes IB, Rush CM, Grassia G, Maffia P, Jenkinson EJ, White AJ, Anderson G, Brewer JM, Garside P,
Journal:Blood
PubMed ID:22271449
'In vivo imaging has revolutionized understanding of the spatiotemporal complexity that subserves the generation of successful effector and regulatory immune responses. Until now, invasive surgery has been required for microscopic access to lymph nodes (LNs), making repeated imaging of the same animal impractical and potentially affecting lymphocyte behavior. To allow ... More
Variables influencing interactions of untargeted quantum dot nanoparticles with skin cells and identification of biochemical modulators.
Authors:Ryman-Rasmussen JP, Riviere JE, Monteiro-Riviere NA
Journal:Nano Lett
PubMed ID:17408303
Skin cells (NHEK) take up untargeted quantum dots (QD) with surface polyethylene glycol (PEG), amines, and carboxylic acids, but the mechanisms are unknown. Time courses of QD-NHEK interactions were determined and effects of QD surface coating, temperature, culture medium supplements and inhibitors of the cell cycle and endocytosis identified. The ... More
Granule exocytosis is required for platelet spreading: differential sorting of a-granules expressing VAMP-7.
Authors:Peters CG, Michelson AD, Flaumenhaft R,
Journal:Blood
PubMed ID:22589474
There has been recent controversy as to whether platelet a-granules represent a single granule population or are composed of different subpopulations that serve discrete functions. To address this question, we evaluated the localization of vesicle-associated membrane proteins (VAMPs) in spread platelets to determine whether platelets actively sort a specific subpopulation ... More
Surface coatings determine cytotoxicity and irritation potential of quantum dot nanoparticles in epidermal keratinocytes.
Authors:Ryman-Rasmussen JP, Riviere JE, Monteiro-Riviere NA
Journal:J Invest Dermatol
PubMed ID:16902417
Quantum dot (QD) nanoparticles have potential applications in nanomedicine as drug delivery vectors and diagnostic agents, but the skin toxicity and irritation potential of QDs are unknown. Human epidermal keratinocytes (HEKs) were used to assess if QDs with different surface coatings would cause differential effects on HEK cytotoxicity, proinflammatory cytokine ... More
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