Flp-In™-293 Cell Line
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Citas y referencias (11)
Invitrogen™

Flp-In™-293 Cell Line

La línea celular Flp-In™ 293 está diseñada para la generación rápida de líneas celulares estables que garantizan la expresión deMás información
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Número de catálogoCantidad
R750071 mL
Número de catálogo R75007
Precio (CLP)
-
Cantidad:
1 mL
La línea celular Flp-In™ 293 está diseñada para la generación rápida de líneas celulares estables que garantizan la expresión de alto nivel de la proteína de interés a partir de un vector de expresión Flp-In™. Estas células contienen un solo sitio de FRT integrado de forma estable en un locus genómico activo transcripcionalmente. La integración selectiva del vector de expresión de Flp-In™ garantiza la expresión de alto nivel del gen de interés. La línea celular Flp-In-293™ se creó mediante la transfección de las líneas celulares madre con pFRT⁄lacZeo y la selección de clones estables resistentes a Zeocin™.

Las células Flp-In™-293 funcionan bien con vectores Flp-In™ que expresan un gen del promotor del CMV (por ejemplo, pcDNA™5⁄FRT, pcDNA5⁄FRT⁄V5-His-TOPO™ y pSecTag⁄FRT⁄V5-His-TOPO
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
N.° de celdas1 mL
Línea de productosFlp-In
Cantidad1 mL
Línea de célulasFlp-In™-293
EspecieHumano
Unit SizeEach
Contenido y almacenamiento
1 x 10E7 células se suministran congeladas en 1 ml de 90 % de medio completo y 10 % de DMSO. Las células deben almacenarse en nitrógeno líquido. Se garantiza la estabilidad de todas las células durante 6 meses si se almacenan adecuadamente.

Preguntas frecuentes

Is multiple integration of the Flp-In expression construct possible? How do you screen for multiple integrants, and how stable is the Flp-In expression cell line?

In theory, one can get multiple integrations of the Flp-In expression construct—an FRT-specific integration event and a random, second-site integration. However, random integration is a relatively uncommon event. Limiting the amount of DNA in the transfection will reduce the chance of second-site integration. We have transfected 293 cells (lacking the FRT site) with the pcDNA5/FRT vector and have identified one potential second-site integrant after screening over 200 clones. DNA integrations can be detected by Southern blot. A single integrant will display a single band; double: two; triple: three, etc. We have maintained a number of Flp-In expression cell lines for over four months and have not observed any loss of the Flp-In expression construct, whether hygromycin selection was maintained or not.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What kind of Flp-In T-REx cell lines do you offer?

We offer the Flp-In T-REx system that contains pFRT/lacZeo and pcDNA6/TR stably integrated into HEK 293 cells. This cell line has been functionally tested for its ability to regulate expression.

Citations & References (11)

Citations & References
Abstract
Functional validation of the genetic polymorphisms of human ATP-binding cassette (ABC) transporter ABCG2: identification of alleles that are defective in porphyrin transport.
Authors:Tamura A, Watanabe M, Saito H, Nakagawa H, Kamachi T, Okura I, Ishikawa T,
Journal:Mol Pharmacol
PubMed ID:16608919
'The ATP-binding cassette (ABC) transporter ABCG2 has been implicated to play a significant role in the response of patients to medication and/or the risk of diseases. To clarify the possible physiological or pathological relevance of ABCG2 polymorphisms, we have functionally validated single nucleotide polymorphisms (SNP) of ABCG2. In the present ... More
Analysis of NOD2-mediated Proteome Response to Muramyl Dipeptide in HEK293 Cells.
Authors:Weichart D, Gobom J, Klopfleisch S, Häsler R, Gustavsson N, Billmann S, Lehrach H, Seegert D, Schreiber S, Rosenstiel P,
Journal:J Biol Chem
PubMed ID:16257953
'NOD2, a cytosolic receptor for the bacterial proteoglycan fragment muramyl dipeptide (MDP), plays an important role in the recognition of intracellular pathogens. Variants in the bacterial sensor domain of NOD2 are genetically associated with an increased risk for the development of Crohn disease, a human chronic inflammatory bowel disease. In ... More
Expression, processing, and glycosaminoglycan binding activity of the recombinant human 315-kDa hyaluronic acid receptor for endocytosis (HARE).
Authors:Harris EN, Kyosseva SV, Weigel JA, Weigel PH,
Journal:J Biol Chem
PubMed ID:17145755
'The hyaluronic acid (HA) receptor for endocytosis (HARE; also designated stabilin-2 and FEEL-2) mediates systemic clearance of glycosaminoglycans from the circulatory and lymphatic systems via coated pit-mediated uptake. HARE is primarily found as two isoforms (315- and 190-kDa) in sinusoidal endothelial cells of the liver, lymph node, and spleen. Here ... More
Hepatitis C virus subgenomic replicons in the human embryonic kidney 293 cell line.
Authors:Ali S, Pellerin C, Lamarre D, Kukolj G,
Journal:J Virol
PubMed ID:14671129
'Hepatitis C virus (HCV) infects liver cells and its replication in other cells is incompletely defined. Human hepatoma Huh-7 cells harboring subgenomic HCV replicons were used in somatic cell fusion experiments with human embryonic kidney 293 cells as a means of examining the permissiveness of 293 cells for HCV subgenomic ... More
Acylation of CD44 and its association with lipid rafts are required for receptor and hyaluronan endocytosis.
Authors:Thankamony SP, Knudson W,
Journal:J Biol Chem
PubMed ID:16945930
CD44 is a cell surface receptor for the extracellular matrix macromolecule hyaluronan. In addition, CD44 mediates the endocytosis of hyaluronan leading to its subsequent degradation within lysosomes. Using model systems of COS-7 and Flp-293 cells, we demonstrate that the association of CD44 with lipid rafts is essential for the endocytosis ... More
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