El kit de soluciones iBind se utiliza con el dispositivo iBind Western que es un dispositivo de procesamiento de WesternMás información
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Número de catálogo
Cantidad
SLF1020X4
Suficiente para 10 reacciones de minimembrana
SLF1020
1 Kit
2 opciones
Número de catálogo SLF1020X4
Precio (CLP)
370.062
4 kits,1 kit
Añadir al carro de la compra
Cantidad:
Suficiente para 10 reacciones de minimembrana
Precio (CLP)
370.062
4 kits,1 kit
Añadir al carro de la compra
El kit de soluciones iBind se utiliza con el dispositivo iBind Western que es un dispositivo de procesamiento de Western automatizado que realiza todos los pasos del flujo de trabajo de Western, desde el bloqueo y el lavado hasta la incubación de anticuerpos. Las soluciones iBind actúan como soluciones de bloqueo, lavado y dilución en el proceso de inmunodetección iBind y se han optimizado para tener una viscosidad ideal. Cada solución pasa de los pocillos del dispositivo iBind a la tarjeta iBind mediante el flujo lateral secuencial (SLF); seguidamente, las soluciones se transfieren hacia la zona de la pila de la tarjeta. La matriz de fibra de vidrio de la tarjeta permite el flujo uniforme y homogéneo de las soluciones hacia la membrana, lo que aumenta la interacción entre el antígeno y el anticuerpo.
El kit de soluciones de iBind, junto con el dispositivo iBind Western y las tarjetas iBind, aportan automatización y simplicidad al protocolo occidental. Cada kit de soluciones iBind contiene suficiente solución para 10 reacciones de minimembrana. Para usar este kit es necesario un dispositivo iBind Western. Para aquellas aplicaciones que emplean métodos fluorescentes, utilice el kit de solución de detección fluorescente (FD) iBind.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
What procedure should I follow when stripping and reprobing my blot processed using the iBind Western Device or iBind Flex Western Device?
To strip and reprobe your blot processed using the iBind Western Device or iBind Flex Western Device, first strip the blot outside the system using your preferred protocol. After the stripping protocol, wash the blot in distilled water for 5 min. Reblocking is unnecessary as the iBind Solution will perform this step during reprobing. Finally, reprobe with new antibodies using the iBind Western Device or iBind Flex Western Device, starting with the membrane preparation step.
Can I multiplex antibodies using the iBind Western Device or iBind Flex Western Device?
Yes, for each midi blot, mini blot, or vertically cut strip, prepare appropriate primary antibodies together in a single tube of iBind FD/ iBind Flex FD Solution, Prepare appropriate secondary antibodies together in a different tube of iBind FD/ iBind Flex FD Solution. Do not mix primary and secondary antibodies prior to loading in the device. All primary and secondary antibodies should be diluted according to the recommendations outlined in the user manual or quick reference card. When detecting several targets of the same species, the same concentration of secondary antibody can be used as when detecting one target. There is no need to increase the concentration for each additional target. Load multiplexed antibodies into the device as normal.
Caution: Consider cross-reactivity of secondary antibodies when multiplexing (e.g., rabbit anti-goat IgG and goat anti-mouse IgG are likely to cross-react).
With the iBind Western Device and iBind Flex Western Device, how do I know if I am rolling the membrane firmly enough?
When firmly rolling, the iBind/iBind Flex Card will slightly dip in the membrane area, causing the top and bottom of the card to lift. See the iBind/iBind Flex user manual for additional details.
Is there any difference between the iBind Flex Card and the original iBind Card?
Besides being ~1.7 times as wide, the iBind Flex Card also includes alignment lines printed on the back that appear when solution is added to the card to help in proper horizontal placement of multiple mini or vertically cut strip blots.