iBind™ Flex Western Starter Kit
iBind™ Flex Western Starter Kit
Invitrogen™

iBind™ Flex Western Starter Kit

El kit iBind Western Starter Kit incluye un dispositivo iBind Flex Western, una caja de tarjetas iBind Flex y unMás información
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Número de catálogoCantidad
SLF2000S1 kit
Número de catálogo SLF2000S
Precio (CLP)
-
Cantidad:
1 kit
El kit iBind Western Starter Kit incluye un dispositivo iBind Flex Western, una caja de tarjetas iBind Flex y un iBind Flex Solution Kit.

El dispositivo iBind Flex Western es un dispositivo automatizado de procesamiento Western que realiza cada paso, desde el bloqueo, pasando por los lavados y hasta las incubaciones de anticuerpos a través del flujo lateral secuencial (SLF). El SLF permite la liberación y flujo oportunos de soluciones y anticuerpos a la membrana sin necesidad de una fuente de alimentación externa, agitadores o bandejas. Cada solución se libera desde los pocillos del dispositivo iBind Flex a una tarjeta iBind Flex a través del SLF; las soluciones se transfieren hacia la región de pila de la tarjeta. La matriz de fibra de vidrio de la tarjeta permite un flujo uniforme y homogéneo de las soluciones hacia la membrana, lo que aumenta la interacción antígeno-anticuerpo.

Más información sobre los sistemas iBind Western ›

El procedimiento de detección iBind Flex Western minimiza el tiempo de intervención y es altamente reproducible. El tiempo de configuración es rápido: en 2,5 h está listo para el paso de detección. En comparación con los métodos manuales, el sistema iBind Flex Western requiere menos anticuerpo primario, permite resultados reproducibles y es compatible con los protocolos de detección posteriores.

El dispositivo iBind Flex Western incluye tres insertos de pocillos intercambiables por el usuario para procesar hasta un midi blot, dos miniblots o seis blots de tiras cortadas verticalmente cuando se utilizan con las tarjetas iBind Flex y el kit de solución iBind Flex o el iBind Flex de solución de detección fluorescente.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Capacidadun midiblot, seis transferencias en tiras cortadas verticalmente, dos miniblots
Contenido del kit10 tarjetas iBind, 1 kit de soluciones iBind, Dispositivo Western
Cantidad1 kit
Tiempo de actividad∼ 2,5 horas
Condiciones de envíoTemperatura ambiente
Método de detecciónCromogénico, quimioluminiscente, fluorescente
Membrane CompatibilityNitrocelulosa, PVDF
Línea de productosiBind
Tipo de productoKit de inicio
Unit Size1 kit
Contenido y almacenamiento
• 1 Dispositivo iBind Flex Western
• 10 Tarjetas iBind Flex, almacenar a temperatura ambiente.
• 1 kit de solución iBind Flex, almacenar a 2–8 °C.

Preguntas frecuentes

Can I use conjugated primary antibodies with the iBind Flex Western Device?

Yes. When using a conjugated primary antibody and no secondary antibody, add the diluted primary antibody to well 1 and replace diluted secondary antibody (well 3) with 1X iBind Flex/iBind Flex FD Solution. Alternatively, for faster time to results, add the diluted primary antibody to well 1, leave wells 2 and 3 empty, and add 1X iBind Flex/ iBind Flex FD Solution to well 4. This procedure will save time (~60 min) but may result in higher background.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What procedure should I follow when stripping and reprobing my blot processed using the iBind Western Device or iBind Flex Western Device?

To strip and reprobe your blot processed using the iBind Western Device or iBind Flex Western Device, first strip the blot outside the system using your preferred protocol. After the stripping protocol, wash the blot in distilled water for 5 min. Reblocking is unnecessary as the iBind Solution will perform this step during reprobing. Finally, reprobe with new antibodies using the iBind Western Device or iBind Flex Western Device, starting with the membrane preparation step.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I multiplex antibodies using the iBind Western Device or iBind Flex Western Device?

Yes, for each midi blot, mini blot, or vertically cut strip, prepare appropriate primary antibodies together in a single tube of iBind FD/ iBind Flex FD Solution, Prepare appropriate secondary antibodies together in a different tube of iBind FD/ iBind Flex FD Solution. Do not mix primary and secondary antibodies prior to loading in the device. All primary and secondary antibodies should be diluted according to the recommendations outlined in the user manual or quick reference card. When detecting several targets of the same species, the same concentration of secondary antibody can be used as when detecting one target. There is no need to increase the concentration for each additional target. Load multiplexed antibodies into the device as normal.
Caution: Consider cross-reactivity of secondary antibodies when multiplexing (e.g., rabbit anti-goat IgG and goat anti-mouse IgG are likely to cross-react).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the iBind Western Device or iBind Flex Western Device with Alexa Fluor Plus conjugated secondary antibodies?

Yes, both the iBind Western Device and iBind Flex Western Device work well with Alexa Fluor Plus conjugated secondary antibodies.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

With the iBind Western Device and iBind Flex Western Device, how do I know if I am rolling the membrane firmly enough?

When firmly rolling, the iBind/iBind Flex Card will slightly dip in the membrane area, causing the top and bottom of the card to lift. See the iBind/iBind Flex user manual for additional details.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.