TC-ReAsH™ II In-Cell Tetracysteine Tag Detection Kit (Red Fluorescence), for live-cell imaging
Citas y referencias (36)
Invitrogen™
TC-ReAsH™ II In-Cell Tetracysteine Tag Detection Kit (Red Fluorescence), for live-cell imaging
El kit de detección de etiqueta de tetracisteína en las células TC-ReAsH™ II contiene un reactivo de etiquetado de fluorescenciaMás información
Have Questions?
Número de catálogo
Cantidad
T34562
1 kit
Número de catálogo T34562
Precio (CLP)
1.553.234
1 kit
Añadir al carro de la compra
Cantidad:
1 kit
Precio (CLP)
1.553.234
1 kit
Añadir al carro de la compra
El kit de detección de etiqueta de tetracisteína en las células TC-ReAsH™ II contiene un reactivo de etiquetado de fluorescencia basado en etiquetas de expresión para el etiquetado de células vivas. Las líneas de células de mamíferos que expresan una proteína fusionada con una etiqueta de tetracisteína (CCPGCC) pueden etiquetarse con el reactivo ReAsH-EDT2 fluorescente rojo incluido en el kit. La proteína etiquetada es fluorescente solo cuando se añade el reactivo de etiquetado. El tampón de lavado BAL sustituye al Disperse Blue 3 y al tampón de lavado a base de EDT suministrados anteriormente como un reactivo olfativamente más agradable que produce una relación señal-ruido superior.
El kit contiene reactivo de etiquetado ReAsH-EDT2 (almacenar a -20 °C, protegido de la luz) y tampón de lavado BAL (almacenar a 4 °C). Cuando se almacena como se indica, el kit es estable durante 6 meses.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
ColorRojo
Método de detecciónFluorescente
Para utilizar con (equipo)Microscopio de fluorescencia
Etiqueta o tinteReAsH
Línea de productosTC-ReAsH II
Tipo de productoKit de detección de etiquetas de tetracisteína
Cantidad1 kit
Condiciones de envíoHielo húmedo
FormatoKit
Unit Size1 kit
Contenido y almacenamiento
Almacenar en congelador (de -5 a -30 °C).
Preguntas frecuentes
Can fluorescent protein-expressing cells be fixed?
Yes, fluorescent protein-expressing cells can be fixed using 4% paraformaldehyde in PBS for 10 min followed by one quick PBS rinse and 3 x 5 min washes with 1 mL PBS.
Are the fluorescent proteins offered by Thermo Fisher Scientific (EmGFP, YFP, CFP, BFP and Cycle 3 GFP) humanized?
Yes, all of the fluorescent proteins offered by (EmGFP, YFP, CFP, BFP and Cycle 3 GFP) have been humanized for optimal mammalian expression.
Citations & References (36)
Citations & References
Abstract
Resolution of de novo HIV production and trafficking in immature dendritic cells.
Authors:Turville SG, Aravantinou M, Stössel H, Romani N, Robbiani M,
Journal:Nat Methods
PubMed ID:18059278
'The challenge in observing de novo virus production in human immunodeficiency virus (HIV)-infected dendritic cells (DCs) is the lack of resolution between cytosolic immature and endocytic mature HIV gag protein. To track HIV production, we developed an infectious HIV construct bearing a diothiol-resistant tetracysteine motif (dTCM) at the C terminus ... More
Identification of an intracellular trafficking and assembly pathway for HIV-1 gag.
Authors:Perlman M, Resh MD
Journal:Traffic
PubMed ID:16683918
'Retroviral Gag proteins are membrane-bound polyproteins that are necessary and sufficient for virus-like particle (VLP) formation. It is not known how Gag traffics through the cell or how the site of particle production is determined. Here we use two techniques, biarsenical/tetracysteine (TC) labeling and release from a cycloheximide block, to ... More
Real-time visualization of HIV-1 GAG trafficking in infected macrophages.
Authors:Gousset K, Ablan SD, Coren LV, Ono A, Soheilian F, Nagashima K, Ott DE, Freed EO,
Journal:PLoS Pathog
PubMed ID:18369466
'HIV-1 particle production is driven by the Gag precursor protein Pr55(Gag). Despite significant progress in defining both the viral and cellular determinants of HIV-1 assembly and release, the trafficking pathway used by Gag to reach its site of assembly in the infected cell remains to be elucidated. The Gag trafficking ... More
Site-specific, orthogonal labeling of proteins in intact cells with two small biarsenical fluorophores.
Authors:Zürn A, Klenk C, Zabel U, Reiner S, Lohse MJ, Hoffmann C,
Journal:Bioconjug Chem
PubMed ID:20429545
'The fusion of fluorescent proteins to proteins of interest has greatly advanced fluorescence microscopy, but is often limited by their large size. Here, we report site-specific, orthogonal labeling of two cellular proteins in intact cells with two small fluorescent dyes: fluorescein arsenical hairpin binder, FlAsH, and its red analogue, ReAsH, ... More
Fluorescence imaging of amyloid formation in living cells by a functional, tetracysteine-tagged alpha-synuclein.
'Alpha-synuclein is a major component of intraneuronal protein aggregates constituting a distinctive feature of Parkinson disease. To date, fluorescence imaging of dynamic processes leading to such amyloid deposits in living cells has not been feasible. To address this need, we generated a recombinant alpha-synuclein (alpha-synuclein-C4) bearing a tetracysteine target for ... More