Search
Citas y referencias (19)
Invitrogen™
Transferrin From Human Serum, Alexa Fluor™ Conjugate
Transferrin is a monomeric serum glycoprotein that binds to a specific receptor on the surface of vertebrate cells and delivers up to two Fe3+ atoms via receptor-mediated endocytosis—our labeled LDL complexes are useful tools for studying this phenomenon.
Have Questions?
Cambiar vista
| Número de catálogo | Tipo de colorante | Cantidad |
|---|---|---|
| T35352 | Colorantes Alexa Fluor | 5 mg |
| T13342 | Colorantes Alexa Fluor | 5 mg |
| T2871 | Colorantes clásicos | 5 mg |
| T23364 | Colorantes Alexa Fluor | 5 mg |
| T23365 | Colorantes Alexa Fluor | 5 mg |
| T13343 | Colorantes Alexa Fluor | 5 mg |
| T23362 | Colorantes Alexa Fluor | 5 mg |
| T23366 | Colorantes Alexa Fluor | 5 mg |
Número de catálogo T35352
Precio (CLP)
634.032
5 mg
Tipo de colorante:
Colorantes Alexa Fluor
Cantidad:
5 mg
Precio (CLP)
634.032
5 mg
Fluorescent transferrin for receptor-mediated endocytosis and recycling
Fluorescent transferrin conjugates are widely used to study transferrin receptor-mediated endocytosis, endosomal trafficking, and recycling using live-cell imaging or fixed-cell endpoint analysis. Transferrin binds transferrin receptor 1 (TfR1) at the cell surface and is internalized through clathrin-mediated endocytosis. Following endosomal acidification and iron release, apo-transferrin remains bound to TfR1 and the complex recycles to the plasma membrane, where apo-transferrin dissociates.
Why use fluorescent transferrin for endocytosis studies?
- Track receptor-mediated endocytosis: Follow transferrin binding, TfR-mediated internalization, and intracellular trafficking.
- Monitor endocytic recycling: Use fluorescent transferrin as a well-established marker of the endosomal recycling pathway.
- Study trafficking kinetics: Follow uptake and recycling using live-cell imaging, pulse-chase experiments, or fixed-cell endpoint analysis.
- Assess endosomal trafficking: Investigate trafficking events associated with endosomal acidification, cargo sorting, and membrane transport.
- Compare trafficking pathways: Combine fluorescent transferrin with fluorescent LDL or other cargo markers to compare recycling and lysosomally directed pathways.
- Enable multiplex fluorescence imaging: Choose from fluorescein and Alexa Fluor™ 488, 546, 555, 568, 594, 633, and 647 conjugates for multicolor imaging workflows.
Fluorescent transferrin enables a direct fluorescence readout of a well-characterized endocytic pathway, making it a versatile probe for studying receptor internalization, endosomal trafficking, and recycling.
Choose a fluorescent transferrin conjugate for your imaging workflow
Select from fluorescein and Alexa Fluor™ 488, 546, 555, 568, 594, 633, and 647 transferrin conjugates, with fluorescence spanning green to far-red wavelengths.
Multiple fluorophore options
- Match your imaging system: Select a conjugate compatible with available excitation sources, emission filters, and detectors.
- Support multicolor imaging: Choose spectrally distinct labels for use with antibodies, organelle markers, and other fluorescent probes.
- Optimize experimental design: Select the excitation and emission profile that best fits your available fluorescence channels.
- Use one biological probe across workflows: Study transferrin receptor trafficking with fluorophore options suitable for a range of fluorescence microscopy applications.
Applications of fluorescent transferrin
Fluorescent transferrin is a well-established probe for studying transferrin receptor-mediated endocytosis and intracellular trafficking.
Use fluorescent transferrin to:
- Visualize receptor-mediated internalization and recycling using live-cell imaging or fixed-cell endpoint analysis
- Track endosomal trafficking using fluorescence and confocal microscopy
- Study trafficking events associated with endosomal acidification and changes in endosomal function
- Investigate transferrin receptor dynamics, including appropriately designed FRET-based studies
- Measure transferrin receptor binding in mammalian and parasite model systems
- Compare recycling and lysosomal trafficking using complementary fluorescent cargo markers
For Research Use Only. Not for use in diagnostic procedures.
Especificaciones
Método de detecciónFluorescente
Tipo de coloranteColorantes Alexa Fluor
Excitación/emisión555/565
FormularioSólido
Familia de proteínasTransferrina
Cantidad5 mg
Condiciones de envíoTemperatura ambiente
Línea de productosAlexa Fluor
Tipo de productoTransferrina
pH7,2
Unit Size5 mg
Contenido y almacenamiento
Almacenar en el congelador (de – 5 a – 30 °C) y proteger de la luz.
Citations & References (19)
Citations & References
Abstract
TrkA receptor activation by nerve growth factor induces shedding of the p75 neurotrophin receptor followed by endosomal gamma-secretase-mediated release of the p75 intracellular domain.
Journal:J Biol Chem
PubMed ID:17215246
'Neurotrophins are trophic factors that regulate important neuronal functions. They bind two unrelated receptors, the Trk family of receptor-tyrosine kinases and the p75 neurotrophin receptor (p75). p75 was recently identified as a new substrate for gamma-secretase-mediated intramembrane proteolysis, generating a p75-derived intracellular domain (p75-ICD) with signaling capabilities. Using PC12 cells
VAMP4 cycles from the cell surface to the trans-Golgi network via sorting and recycling endosomes.
Journal:J Cell Sci
PubMed ID:17327277
'VAMP4 is enriched in the trans-Golgi network (TGN) and functions in traffic from the early and recycling endosomes to the TGN, but its trafficking itinerary is unknown. Cells stably expressing TGN-enriched VAMP4 C-terminally-tagged with EGFP (VAMP4-EGFP) are able to internalize and transport EGFP antibody efficiently to the TGN, suggesting that
Elucidation of intracellular recycling pathways leading to exocytosis of the Fc receptor, FcRn, by using multifocal plane microscopy.
Journal:Proc Natl Acad Sci U S A
PubMed ID:17384151
'The intracellular events on the recycling pathway that lead from sorting endosomes to exocytosis at the plasma membrane are central to cellular function. However, despite intensive study, these processes are poorly characterized in spatial and dynamic terms. The primary reason for this is that, to date, it has not been
Small GTPase Rab21 regulates cell adhesion and controls endosomal traffic of beta1-integrins.
Journal:J Cell Biol
PubMed ID:16754960
'Dynamic turnover of integrin cell adhesion molecules to and from the cell surface is central to cell migration. We report for the first time an association between integrins and Rab proteins, which are small GTPases involved in the traffic of endocytotic vesicles. Rab21 (and Rab5) associate with the cytoplasmic domains
High accuracy 3D quantum dot tracking with multifocal plane microscopy for the study of fast intracellular dynamics in live cells.
Journal:Biophys J
PubMed ID:18835896
'Single particle tracking in three dimensions in a live cell environment holds the promise of revealing important new biological insights. However, conventional microscopy-based imaging techniques are not well suited for fast three-dimensional (3D) tracking of single particles in cells. Previously we developed an imaging modality multifocal plane microscopy (MUM) to