FM™ 1-43 Dye (N-(3-Triethylammoniumpropyl)-4-(4-(Dibutylamino) Styryl) Pyridinium Dibromide)
FM&trade; 1-43 Dye (<i>N</i>-(3-Triethylammoniumpropyl)-4-(4-(Dibutylamino) Styryl) Pyridinium Dibromide)
Citas y referencias (371)
Invitrogen™

FM™ 1-43 Dye (N-(3-Triethylammoniumpropyl)-4-(4-(Dibutylamino) Styryl) Pyridinium Dibromide)

La sonda de membranas FM 1-43 es un excelente reactivo que se utiliza ampliamente para identificar activamente neuronas emisoras yMás información
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Número de catálogoCantidad
T3535610 x 100 μg
T31631mg
Número de catálogo T35356
Precio (CLP)
588.119
10 x 100 µg
Añadir al carro de la compra
Cantidad:
10 x 100 μg
Precio (CLP)
588.119
10 x 100 µg
Añadir al carro de la compra
La sonda de membranas FM 1-43 es un excelente reactivo que se utiliza ampliamente para identificar activamente neuronas emisoras y para investigar los mecanismos de los ciclos vesiculares dependientes de la actividad. Este tinte soluble en agua, que no es tóxico para las células y prácticamente no fluorescente en medio acuoso, se cree que se inserta en el recubrimiento exterior de la membrana celular donde se vuelve intensamente fluorescente. En una neurona que está liberando neurotransmisores activamente, el tinte se introduce dentro de las vesículas sinápticas recicladas y los terminales nerviosos se tiñen con brillo. La tinción inespecífica de las membranas de la superficie celular se puede lavar simplemente antes de la visualización. La sonda de membrana FM 1-43 también está disponible como 1 mg en un solo vial (T-3163). También está disponible la sonda de membrana FM 1-43FX (F-35355), un analógico de la sonda de membrana FM 1-43 que se puede fijar en su lugar mediante fijadores a base de aldehído.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
ColorRojo
Método de detecciónFluorescente
Para utilizar con (equipo)Microscopio de fluorescencia
Línea de productosFM
Cantidad10 x 100 μg
Condiciones de envíoTemperatura ambiente
Tipo de etiquetaFluorescent Dye
Tipo de productoTinte
SubCellular LocalizationMembrana plasmática
Unit Size10 x 100 µg
Contenido y almacenamiento
Almacenar a temperatura ambiente y proteger de la luz.

Preguntas frecuentes

I want to study endosomes trafficking using FM 4-64. Will the label be retained after fixation? And can I label already-fixed cells?

No. For that you would need the FM 4-64FX version. The non-FX version will not be retained upon fixation, leading to loss of much of the stain and an increase in background. The FX version will be retained using an aldehyde-based fixative. Cells that are already fixed will be stained throughout the cell and the signal will not be localized; it is recommended to stain live cells and then fix.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to study endosome trafficking using FM 4-64 dye. Will the label be retained after fixation? And can I label cells that have already been fixed?

No. For that, you would need the FM 4-64FX version. The non-FX version will be lost, leading to loss of much of the specific label and a vast increase in background labeling. The FX version will be fixed in place with formaldehyde. Cells that have been fixed already will not label correctly, so you will need to label the cells live and then fix.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

The product sheet for FM 1-43 Dye states that the maximum for excitation is 510 nm and emissions is 626 nm, but the spectrum on the product page shows a peak at 470 nm and 580 nm. Can you clarify what the excitation and emission maxima is for this dye?

FM lipophilic styryl dyes are known to shift their spectral properties upon binding to membranes. These dyes exhibit an appreciable solvent and environmental dependency in both free and membrane-bound forms. The emissions of the membrane-bound dye shifts to a shorter wavelength compared to the emission in the solvent.

In solvent, FM 1-43 Dye (Cat. Nos. T35356, T3163, F35355, F10317) absorbs in the range of fluorescein (~490 nm) and initially emits at around 626-636 nm. However, upon membrane integration, it exhibits emission in the 565-590 nm range. Because this dye exhibits a large Stokes shift, it does not perfectly fit into a green or orange fluorophore profile, but is rather a yellow-fluorescent dye, and may require a long-pass emission filter.

Note: FM 1-43 Dye is often used with typical fluorescein or GFP optical filter sets for biological imaging but is poorly excited through tetramethylrhodamine filters.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (371)

Citations & References
Abstract
Clathrin-mediated endocytosis near active zones in snake motor boutons.
Authors:Teng H,Wilkinson RS
Journal:The Journal of neuroscience : the official journal of the Society for Neuroscience
PubMed ID:11050119
We have used the activity-dependent probe FM1-43 with electron microscopy (EM) to examine endocytosis at the vertebrate nerve–muscle synapse. Preparations were fixed after very brief neural stimulation at reduced temperature, and internalized FM1-43 was photoconverted into an electron-dense reaction product. To locate the reaction product, we reconstructed computer renderings of ... More
Properties of fast endocytosis at hippocampal synapses.
Authors:Kavalali ET,Klingauf J,Tsien RW
Journal:Philosophical transactions of the Royal Society of London. Series B, Biological sciences
PubMed ID:10212482
Regulation of synaptic transmission is a widespread means for dynamic alterations in nervous system function. In several cases, this regulation targets vesicular recycling in presynaptic terminals and may result in substantial changes in efficiency of synaptic transmission. Traditionally, experimental accessibility of the synaptic vesicle cycle in central neuronal synapses has ... More
Endosome fusion and microtubule-based dynamics in the early endocytic pathway of dictyostelium.
Authors:Clarke M, Köhler J, Heuser J, Gerisch G
Journal:Traffic
PubMed ID:12383345
Dictyostelium amoebae, like mammalian macrophages, take up fluid by macropinocytosis. The present study used fluorescent fluid-phase markers and GFP-labeled microtubules to visualize the uptake, dynamics, and fusion of early endosomes in Dictyostelium. Consecutive labeling with two fluorescent fluid-phase markers demonstrated that within the first few minutes after uptake, new macropinosomes ... More
Reticulated lipid probe fluorescence reveals MDCK cell apical membrane topography.
Authors:Colarusso P, Spring KR
Journal:Biophys J
PubMed ID:11806917
High spatial resolution confocal microscopy of young MDCK cells stained with the lipophilic probe 1,1'-dihexadecyl-3,3,3',3'- tetramethylindocarbocyanine perchlorate (DiIC(16)) revealed a reticulated fluorescence pattern on the apical membrane. DiIC(16) was delivered as crystals to live cells to minimize possible solvent perturbations of the membrane lipids. The ratio of the integrated fluorescence ... More
Visualization of changes in presynaptic function during long-term synaptic plasticity.
Authors:Zakharenko SS, Zablow L, Siegelbaum SA
Journal:Nat Neurosci
PubMed ID:11426227
Controversy exists regarding the site of modification of synaptic transmission during long-term plasticity in the mammalian hippocampus. Here we used a fluorescent marker of presynaptic activity, FM 1-43, to directly image changes in presynaptic function during both short-term and long-term forms of plasticity at presynaptic boutons of CA3-CA1 excitatory synapses ... More
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