TransFluoSpheres™ Carboxylate-Modified Microspheres, 1.0 μm (488/645), 2% solids

Citas y referencias (7)

Invitrogen™

TransFluoSpheres™ Carboxylate-Modified Microspheres, 1.0 μm (488/645), 2% solids

Las microesferas (también llamadas gránulos o partículas de látex) son partículas esféricas en el rango de tamaño coloidal que seMás información

Número de catálogo	Cantidad
T8883	0.5 mL

Número de catálogo T8883

Precio (CLP)

Cantidad:

0.5 mL

Las microesferas (también llamadas gránulos o partículas de látex) son partículas esféricas en el rango de tamaño coloidal que se forman a partir de un polímero amorfo como el poliestireno. Nuestros gránulos Molecular Probes™ TransFluoSpheres™ se fabrican utilizando poliestireno ultralimpio de alta calidad y se cargan con dos (o más) colorantes fluorescentes para crear unas microesferas que pueden ser excitadas por las líneas espectrales de 488 nm o 514 nm del láser de iones de argón, pero emiten a longitudes de onda mucho más largas, facilitando los experimentos multicolores y permitiendo el uso de filtros de excitación y emisión de banda ancha. Las microesferas TransFluoSpheres™ suelen mostrar poco o ningún fotoblanqueo, incluso cuando son excitadas por la intensa iluminación necesaria para la microscopia de fluorescencia.

™ Especificaciones de la microesfera TransFluoSpheres:

• Etiqueta (Ex/Em): Fluorescente carmesí (488/645)
• Diámetro nominal del gránulo: 1,0 µm
• Superficie de acoplamiento: Carboxilato
• Sólidos: 2%

Características de la superficie de acoplamiento de carboxilato
Los gránulos de FluoSpheres™ modificados con carboxilato tienen una alta densidad de ácidos carboxílicos colgantes en su superficie, lo que los hace adecuados para el acoplamiento covalente de proteínas y otras biomoléculas que contienen amina utilizando reactivos de carbodiimida solubles en agua como EDAC.

Aplicaciones principales de las microesferas:
• Calibración de instrumentos (citometría de flujo, microscopia, HTS, HCS)
• Pruebas de flujo (microfluídica, flujo sanguíneo, flujo de agua y flujo de aire)
• Trazadores de biología celular (diferenciación celular y rastreo celular)
• Inmunoensayos (pruebas de aglutinación, ELISA, captura de partículas y reactivos de contraste)

Opciones para microesferas fluorescentes
Entre nuestra completa oferta de productos de microesferas fluorescentes, encontrará gránulos con estas variaciones:
• Diez colores fluorescentes
• Diez diámetros nominales de gránulos: 0,02 µm, 0,04 µm, 0,1 µm, 0,2 µm, 0,5 µm, 1,0 µm, 2,0 µm, 4,0 µm, 10,0 µm y 15,0 µm
• Cuatro modificaciones superficiales para el acoplamiento de proteínas: carboxilato, sulfato, aldehído-sulfato, amina
• Microesferas que además están preacopladas con estreptavidina, neutravidina, biotina, europio y platino

Opciones de microesferas sin teñir
También ofrecemos cientos de opciones de microesferas sin surfactantes UltraClean™ para aplicaciones de investigación y comerciales.

Le prepararemos un producto de microesferas personalizado
Preparamos pedidos personalizados a petición del cliente. Nuestro servicio de conjugación personalizada es eficiente y confidencial, y garantizamos la calidad de nuestro trabajo. Contamos con la certificación ISO 9001:2000.

Solo para uso en investigación. No apto para uso diagnóstico o terapéutico en animales o humanos.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Línea de productosTransFluoSpheres

Cantidad0.5 mL

Condiciones de envíoTemperatura ambiente

Modificación de la superficieCarboxilato

ColorCarmesí

Diámetro (métrico)1 μm

MaterialPoliestireno

Tipo de productoMicroesfera modificada con carboxilatos

Unit Size0.5 mL

Contenido y almacenamiento

Almacenar en el refrigerador (2–8 °C) y proteger de la luz.

Preguntas frecuentes

What is the warranty for FluoSpheres microspheres?

The warranty period for FluoSpheres microspheres is 1-year from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

After washing and centrifugation, there was only a very small pellet left of my microsphere beads and the solution was transparent. Why is this?

Centrifugation is not an effective way to collect smaller microspheres; many particles remain in the solution even if you can visualize a small pellet. For beads less than 1 µm in diameter, we recommend washing by either:

Cross-flow filtration, as these particles have a very high compression modulus and can withstand high g-forces without risk of harm or dialysis with a 500 kDa MWCO
Note: Microspheres greater than 1 µm in diameter can be centrifuged at 1,300 rpm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I've had my microspheres for over a year, and I'm wondering if they're still good to use. What are some good ways to check their functionality?

Bacterial contamination is the most common cause of microspheres becoming unusable. Many of our particles are supplied with a low level of sodium azide to prevent bacterial contamination, but sometimes this can still occur. Bacterial contamination is best assessed by plating on appropriate growth medium and checking the plates after 72 hr.

Find additional tips, troubleshooting help, and resources within ourMicrospheres Support Center.

I accidentally froze my microspheres; can I still use them?

Even brief freezing can cause irreversible aggregation and potential distortion of the bead shape. You should not use these microspheres.

Find additional tips, troubleshooting help, and resources within our Microspheres Support Center.

My protein-coated microspheres appear to be non-specifically binding in my experimental system. Do you have a product that can help reduce these non-specific interactions?

Non-specific binding can often be relieved by a blocking solution, but microspheres seem to require a stronger blocking solution than those most commonly commercially available. Hence, we've developed the BlockAid Blocking Solution (Cat. No. B10710). This reagent is a protein-based blocking solution designed for use with FluoSpheres microspheres and TransFluoSpheres microspheres conjugated to biotin, streptavidin, NeutrAvidin biotin-binding protein, or other proteins. The BlockAid Blocking Solution has proven useful for reducing the nonspecific binding of protein-coated or other macromolecule-coated microspheres in a wide variety of flow cytometry, microscopy, and microarray applications.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all TransFluoSpheres™ Carboxylate-Modified Microspheres, 1.0 μm (488/645), 2% solids FAQs

Citations & References (7)

Citations & References

Abstract

A single amino acid in the cytoplasmic domain of the beta 2 integrin lymphocyte function-associated antigen-1 regulates avidity-dependent inside-out signaling.

Authors:Bleijs DA, van Duijnhoven GC, van Vliet SJ, Thijssen JP, Figdor CG, van Kooyk Y

Journal:J Biol Chem

PubMed ID:11134023

'The leukocyte-specific beta(2) integrin lymphocyte function-associated antigen-1 (LFA-1) (alpha(L)/beta(2)) mediates activation-dependent adhesion to intercellular adhesion molecule (ICAM)-1. In leukocytes, LFA-1 requires activation by intracellular messengers to bind ICAM-1. We observed malfunctioning of LFA-1 activation in leukemic T cells and K562-transfected cells. This defective inside-out integrin activation is only restricted to ... More

DC-SIGN-ICAM-2 interaction mediates dendritic cell trafficking.

Authors:Geijtenbeek TB, Krooshoop DJ, Bleijs DA, van Vliet SJ, van Duijnhoven GC, Grabovsky V, Alon R, Figdor CG, van Kooyk Y

Journal:Nat Immunol

PubMed ID:11017109

'Dendritic cells (DCs) are recruited from blood into tissues to patrol for foreign antigens. After antigen uptake and processing, DCs migrate to the secondary lymphoid organs to initiate immune responses. We now show that DC-SIGN, a DC-specific C-type lectin, supports tethering and rolling of DC-SIGN-positive cells on the vascular ligand ... More

Comparison of methods for monitoring bacterial transport in the subsurface.

Authors:DeFlaun MF, Fuller ME, Zhang P, Johnson WP, Mailloux BJ, Holben WE, Kovacik WP, Balkwill DL, Onstott TC

Journal:J Microbiol Methods

PubMed ID:11576686

'The purpose of this study was to compare in a laboratory experiment, a suite of methods developed to track viable bacteria during field transport experiments. The criteria for development and selection of these methods included: (1) the ability to track bacteria within the environment from which they were isolated; (2) ... More

Identification of DC-SIGN, a novel dendritic cell-specific ICAM-3 receptor that supports primary immune responses.

Authors:Geijtenbeek TB, Torensma R, van Vliet SJ, van Duijnhoven GC, Adema GJ, van Kooyk Y, Figdor CG

Journal:Cell

PubMed ID:10721994

Contact between dendritic cells (DC) and resting T cells is essential to initiate a primary immune response. Here, we demonstrate that ICAM-3 expressed by resting T cells is important in this first contact with DC. We discovered that instead of the common ICAM-3 receptors LFA-1 and alphaDbeta2, a novel DC-specific ... More

DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells.

Authors:Geijtenbeek TB, Kwon DS, Torensma R, van Vliet SJ, van Duijnhoven GC, Middel J, Cornelissen IL, Nottet HS, KewalRamani VN, Littman DR, Figdor CG, van Kooyk Y

Journal:Cell

PubMed ID:10721995

Dendritic cells (DC) capture microorganisms that enter peripheral mucosal tissues and then migrate to secondary lymphoid organs, where they present these in antigenic form to resting T cells and thus initiate adaptive immune responses. Here, we describe the properties of a DC-specific C-type lectin, DC-SIGN, that is highly expressed on ... More

View all TransFluoSpheres™ Carboxylate-Modified Microspheres, 1.0 μm (488/645), 2% solids citations