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Citas y referencias (33)
Invitrogen™
Vybrant™ Cell Adhesion Assay Kit
El kit de ensayo de adherencia celular Vybrant™ es un ensayo rápido y sensible para medir la adherencia de célulaMás información
| Número de catálogo | Cantidad |
|---|---|
| V13181 | 1 kit |
Número de catálogo V13181
Precio (CLP)
530.694
1 kit
Cantidad:
1 kit
Precio (CLP)
530.694
1 kit
El kit de ensayo de adherencia celular Vybrant™ es un ensayo rápido y sensible para medir la adherencia de célula a célula o de célula a superficie para varios tipos celulares. En este ensayo, las células se etiquetan con calceína AM y se les permite adherirse. Después de la eliminación de células no adherentes, la fluorescencia de la calceína se utiliza para calcular el número de células adherentes.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Método de detecciónFluorescente
Tipo de coloranteOtras etiquetas o colorantes
FormatoPlaca de 96 pocillos
Cantidad1 kit
Condiciones de envíoTemperatura ambiente
Para utilizar con (aplicación)Ensayo de fijación celular
Para utilizar con (equipo)Lector de microplacas
Línea de productosVybrant
Tipo de productoTinción
Unit Size1 kit
Contenido y almacenamiento
Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.
Preguntas frecuentes
What are the fluorescence excitation/emission maxima for the Calcein AM and SYTOX Green nucleic acid stain provided in the Vybrant Cell Adhesion Assay Kit?
Citations & References (33)
Citations & References
Abstract
Effect of C-reactive protein on gene expression in vascular endothelial cells.
Journal:Am J Physiol Heart Circ Physiol
PubMed ID:15591095
'C-reactive protein (CRP) is significantly associated with the risk of ischemic cardiovascular disease in epidemiological studies. To explore if CRP has a functional role, we investigated its effect on the gene expression profile of vascular endothelial cells. Human vascular endothelial cells (human umbilical vein endothelial cells and human aortic endothelial
Atherogenic lipids induce adhesion of human coronary artery smooth muscle cells to macrophages by up-regulating chemokine CX3CL1 on smooth muscle cells in a TNFalpha-NFkappaB-dependent manner.
Journal:J Biol Chem
PubMed ID:17456471
'Recent genetic evidence has implicated the adhesive chemokine CX3CL1 and its leukocyte receptor CX3CR1 in atherosclerosis. We previously proposed a mechanism involving foam cell anchorage to vascular smooth muscle cells because: 1) CX3CL1 and CX3CR1 are expressed by both cell types in mouse and human atherosclerotic lesions; 2) foam cells
ROCK inhibitor improves survival of cryopreserved serum/feeder-free single human embryonic stem cells.
Journal:Hum Reprod
PubMed ID:19056770
'Efficient slow freezing protocols within serum-free and feeder-free culture systems are crucial for the clinical application of human embryonic stem (hES) cells. Frequently, however, hES cells must be cryopreserved as clumps when using conventional slow freezing protocols, leading to lower survival rates during freeze-thaw and limiting their recovery and growth
CXCL16 signals via Gi, phosphatidylinositol 3-kinase, Akt, I kappa B kinase, and nuclear factor-kappa B and induces cell-cell adhesion and aortic smooth muscle cell proliferation.
Journal:J Biol Chem
PubMed ID:14625285
'CXCL16, a recently discovered transmembrane chemokine, is expressed in human aortic smooth muscle cell (ASMC). It facilitates uptake of low density lipoproteins by macrophages, resulting in foam cell formation. However, it is not known whether ASMC express CXCR6, the receptor for CXCL16, or whether CXCL16 affects ASMC biology. To dissect
Targeting PKC in multiple myeloma: in vitro and in vivo effects of the novel, orally available small-molecule inhibitor enzastaurin (LY317615.HCl).
Journal:Blood
PubMed ID:17023575
'In multiple myeloma (MM) protein kinase C (PKC) signaling pathways have been implicated in cell proliferation, survival, and migration. Here we investigated the novel, orally available PKC-inhibitor enzastaurin for its anti-MM activity. Enzastaurin specifically inhibits phorbol ester-induced activation of PKC isoforms, as well as phosphorylation of downstream signaling molecules MARCKS