Mitochondrial Membrane Potential Apoptosis Kit, with Mitotracker™ Red & Annexin V Alexa Fluor™ 488, for flow cytometry

Citas y referencias (4)

Invitrogen™

Mitochondrial Membrane Potential Apoptosis Kit, with Mitotracker™ Red & Annexin V Alexa Fluor™ 488, for flow cytometry

Este producto de citometría de flujo proporciona un ensayo rápido y cómodo para dos características distintivas de la apoptosis -Más información

Número de catálogo	Cantidad
V35116	1 Kit

Número de catálogo V35116

Precio (CLP)

591.054

1 kit

Añadir al carro de la compra

Cantidad:

1 Kit

Precio (CLP)

591.054

1 kit

Añadir al carro de la compra

Este producto de citometría de flujo proporciona un ensayo rápido y cómodo para dos características distintivas de la apoptosis - La externalización de fosfatidilserina y los cambios en el potencial de la membrana mitocondrial. Después de teñir una población celular con las tinciones Alexa Fluor™ 488 anexina V y MitoTracker™ Red CMXRos en el tampón de unión, las células vivas exhiben muy poca fluorescencia verde y fluorescencia roja brillante, mientras que las apoptóticas muestran fluorescencia verde y disminución de la fluorescencia roja. Estas poblaciones pueden distinguirse fácilmente mediante un citómetro de flujo; la línea de 488 nm de un láser de iones de argón puede usarse para excitar ambas tinciones.

Consulte la guía de selección para todos los ensayos de apoptosis para citometría de flujo.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Excitación/emisiónMitotracker™ Red 579⁄599, Alexa Fluor™ 488: 499⁄521

Líneas láser del citómetro de flujo488

Para utilizar con (aplicación)Citometría de flujo

Para utilizar con (equipo)Microscopio de fluorescencia, citómetro de flujo

N.º de reacciones50

Línea de productosAlexa Fluor, MitoTracker

Tipo de productoKit de apoptosis de membrana mitocondrial

Cantidad1 Kit

Condiciones de envíoHielo húmedo

ConjugadoAlexa Fluor™ 488, MitoTracker™ Red

FormatoTubo, portaobjetos

Unit Size1 kit

Contenido y almacenamiento

Contiene 1 vial de conjugado de anexina, Alexa Fluor™ 488 (250 µl), 3 viales de MitoTracker™ Red (50 µg por vial), 1 frasco de tampón de unión de anexina (solución 5X, 15 ml) y 1 vial de DMSO (100 µl).

Almacenar en el refrigerador (2–8 °C) y proteger de la luz.

Preguntas frecuentes

What are the fluorescence excitation/emission maxima for the dyes contained in the Mitochondrial Membrane Potential Apoptosis Kit, with Mitotracker Red & Alexa Fluor 488 annexin V, for flow cytometry

Alexa Fluor 488 annexin V: 499/521 nm
MitoTracker Red: 579/599 nm

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to study apoptosis using an Annexin V conjugate, but with adherent cells via microscopy instead of flow cytometry. Can this be done?

It has been done, but we don‘t recommend it. Both healthy cells and apoptotic cells possess phosphatidylserine on the cell surface, which can be detected with Annexin V, but apoptotic cells have significantly more of it. You can easily tell the difference between these two populations with flow cytometry, because flow cytometers are more sensitive and have a higher throughput. But with a microscope, you cannot always tell the difference, especially for adherent cells. Instead, for microscopy, we recommend a different technique, such as detecting caspases with CellEvent Caspase Detection Reagents.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (4)

Citations & References

Abstract

Galectin 1 modulates plasma cell homeostasis and regulates the humoral immune response.

Authors:Anginot A, Espeli M, Chasson L, Mancini SJ, Schiff C,

Journal:J Immunol

PubMed ID:23616571

'Galectin-1 (GAL1) is an S-type lectin with multiple functions, including the control of B cell homeostasis. GAL1 expression was reported to be under the control of the plasma cell master regulator BLIMP-1. GAL1 was detected at the protein level in LPS-stimulated B cells and was shown to promote Ig secretion ... More

FGF23 activates injury-primed renal fibroblasts via FGFR4-dependent signalling and enhancement of TGF-ß autoinduction.

Authors:Smith ER, Holt SG, Hewitson TD

Journal:Int J Biochem Cell Biol

PubMed ID:28919046

Bone-derived fibroblast growth factor 23 (FGF23) is an important endocrine regulator of mineral homeostasis with effects transduced by cognate FGF receptor (FGFR)1-a-Klotho complexes. Circulating FGF23 levels rise precipitously in patients with kidney disease and portend worse renal and cardiovascular outcomes. De novo expression of FGF23 has been found in the ... More

High Tidal Volume Induces Mitochondria Damage and Releases Mitochondrial DNA to Aggravate the Ventilator-Induced Lung Injury.

Authors:Lin JY, Jing R, Lin F, Ge WY, Dai HJ, Pan L

Journal:Front Immunol

PubMed ID:30018615

This study aimed to determine whether high tidal volume (HTV) induce mitochondria damage and mitophagy, contributing to the release of mitochondrial DNA (mtDNA). Another aim of the present study was to investigate the role and mechanism of mtDNA in ventilator-induced lung injury (VILI) in rats. ... More

Adaptive phenotypic modulations lead to therapy resistance in chronic myeloid leukemia cells.

Authors:Baykal-Köse S, Acikgoz E, Yavuz AS, Gönül Geyik Ö, Ates H, Sezerman OU, Özsan GH, Yüce Z

Journal:PLoS One

PubMed ID:32106243

Tyrosine kinase inhibitor (TKI) resistance is a major problem in chronic myeloid leukemia (CML). We generated a TKI-resistant K562 sub-population, K562-IR, under selective imatinib-mesylate pressure. K562-IR cells are CD34-/CD38-, BCR-Abl-independent, proliferate slowly, highly adherent and form intact tumor spheroids. Loss of CD45 and other hematopoietic markers reveal these cells have ... More