pLenti6/V5-DEST™ Gateway™ Vector
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Citas y referencias (4)
Invitrogen™

pLenti6/V5-DEST™ Gateway™ Vector

El vector pLenti6⁄V5-DEST™ Gateway™ es un vector de expresión lentiviral ViraPower™ adaptado para Gateway™ para la expresión lentiviral de unMás información
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Número de catálogoCantidad
V496106 μg
Número de catálogo V49610
Precio (CLP)
-
Cantidad:
6 μg
El vector pLenti6⁄V5-DEST™ Gateway™ es un vector de expresión lentiviral ViraPower™ adaptado para Gateway™ para la expresión lentiviral de un gen diana en células de mamíferos que se dividen y no se dividen. El vector incluye el promotor de CMV para llevar a cabo una expresión constitutiva del gen diana y del marcador de selección de blasticidina y conseguir una selección estable en células de mamíferos.

Ventajas
• Expresión lentiviral de un gen diana en células de mamíferos que se dividen y no se dividen

Características clave
• Tecnología de clonación de recombinación Gateway™ flexible y versátil
• Alto nivel de expresión constitutiva con un promotor de CMV
• Marcador de selección de blasticidina para conseguir una selección estable
• Etiqueta V5 C-terminal para la detección rápida

Contenido del kit
• Vector pLenti6⁄V5-DEST™ Gateway™
• E. coli químicamente competente One Shot™ Stbl3™ (C7373-03)

SKU relacionadas
• Vector pLenti6⁄UbC⁄V5-DEST™ Gateway™ (V49910)
• Vector pLenti4⁄V5-DEST™ Gateway™ (V49810)
• Kit de clonación pLenti6⁄V5 Directional TOPO™ (K4955-10)
• Kit de expresión ViraPower™ Lentiviral Directional TOPO™ (K495000)
• Kit de expresión ViraPower™ Lentiviral Gateway™ (K4960-00)
• Kit de expresión ViraPower™ HiPerform™ Lentiviral Gateway™ (K5330-00)

Para uso exclusivo en investigación. No diseñado para uso diagnóstico o terapéutico.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Sistema constitutivo o inducibleConstitutivo
Tipo de entregaLentivírico
Para utilizar con (aplicación)Expresión viral
Tipo de productoVector de expresión lentiviral
Cantidad6 μg
Agente de selección (eucariótico)Blasticidina
VectorpDEST, pLenti
Método de clonaciónGateway
Línea de productosGateway, ViraPower
PromotorCMV
Etiqueta de proteínaEtiqueta de epítopo V5
Unit Size6 µg
Contenido y almacenamiento
Los vectores ViraPower™ Lentiviral Gateway™ se conservan a –20°C e incluyen:

pLenti6⁄V5-DEST™: 6 μg (40 μl de vector 150 ng⁄μl en 10 mM
Tris-HCl, 1mM EDTA, pH 8.0)

pLenti6⁄V5-GW⁄lacZ control: 10 μg
(20 μl de 0.5 μg⁄μl vector en 10 mM
Tris-HCl, 1mM EDTA, pH 8.0)

E. coli químicamente competente One Shot™ Stbl3™ conservar a –80°C

Preguntas frecuentes

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all pLenti6/V5-DEST™ Gateway™ Vector FAQs

Citations & References (4)

Citations & References
Abstract
The membrane anchor R7BP controls the proteolytic stability of the striatal specific RGS protein, RGS9-2.
Authors:Anderson GR, Semenov A, Song JH, Martemyanov KA,
Journal:J Biol Chem
PubMed ID:17158100
'A member of the RGS (regulators of G protein signaling) family, RGS9-2 is a critical regulator of G protein signaling pathways that control locomotion and reward signaling in the brain. RGS9-2 is specifically expressed in striatal neurons where it forms complexes with its newly discovered partner, R7BP (R7 family binding ... More
West Nile virus discriminates between DC-SIGN and DC-SIGNR for cellular attachment and infection.
Authors:Davis CW, Nguyen HY, Hanna SL, Sánchez MD, Doms RW, Pierson TC,
Journal:J Virol
PubMed ID:16415006
'The C-type lectins DC-SIGN and DC-SIGNR bind mannose-rich glycans with high affinity. In vitro, cells expressing these attachment factors efficiently capture, and are infected by, a diverse array of appropriately glycosylated pathogens, including dengue virus. In this study, we investigated whether these lectins could enhance cellular infection by West Nile ... More
Tumorigenesis suppressor Pdcd4 down-regulates mitogen-activated protein kinase kinase kinase kinase 1 expression to suppress colon carcinoma cell invasion.
Authors:Yang HS, Matthews CP, Clair T, Wang Q, Baker AR, Li CC, Tan TH, Colburn NH,
Journal:Mol Cell Biol
PubMed ID:16449643
Programmed cell death 4 (Pdcd4) suppresses neoplastic transformation by inhibiting the activation of c-Jun and consequently AP-1-dependent transcription. We report that Pdcd4 blocks c-Jun activation by inhibiting the expression of mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1)/hematopoietic progenitor kinase 1, a kinase upstream of Jun N-terminal kinase (JNK). cDNA ... More
Isolation of cell lines that show novel, murine leukemia virus-specific blocks to early steps of retroviral replication.
Authors:Bruce JW, Bradley KA, Ahlquist P, Young JA,
Journal:J Virol
PubMed ID:16188999
In order to identify cellular proteins required for early stages of retroviral replication, a high volume screening with mammalian somatic cells was performed. Ten pools of chemically mutagenized Chinese hamster ovary (CHO-K1) cells were challenged with a murine leukemia virus (MLV) vector pseudotyped with the vesicular stomatitis virus glycoprotein (VSV-G), ... More