Rat (Wistar) Cryopreserved Hepatocytes, Plateable Male

Estos hepatocitos criopreservados plaqueables de ratas macho (Wistar) para cultivo diferencial se han caracterizado para actividades enzimáticas de metabolización deMás información

Número de catálogo	Cantidad
WICP10	1 mL

Número de catálogo WICP10

Precio (CLP)

Cantidad:

Estos hepatocitos criopreservados plaqueables de ratas macho (Wistar) para cultivo diferencial se han caracterizado para actividades enzimáticas de metabolización de fármacos en fase I y fase II.

Células por vial: 4–8 millones

Obtenga los resultados que necesita con los hepatocitos GIBCO
Los hepatocitos de alta calidad con altas viabilidades, los niveles de expresión enzimática in vivo y la morfología celular adecuada aumentan la capacidad de extracciones de correlaciones in vitro⁄in vivo, para tomar decisiones fundadas con respecto al destino de un compuesto. Los técnicos experimentados y las técnicas de aislamiento patentadas junto con las estrictas especificaciones de liberación garantizan la recepción de hepatocitos criopreservados de la mejor calidad.

Cada lote se prueba para:

• Actividades enzimáticas en fase I y II
• Morfología adecuada
• Viabilidad generalmente > 75 %
• Eficacia de los accesorios
• Formación e integridad de monocapas

Solo para uso en investigación. No está indicado para uso terapéutico ni diagnóstico humano o animal.

Enlaces relacionados:

Obtenga más información sobre nuestros hepatocitos criopreservados

Obtenga más información sobre nuestra línea completa de hepatocitos,

Nota:
Estos productos requieren envío en vasos Dewar LN₂, por lo que pueden aplicarse cargos adicionales.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Fuente de dadoresAgrupadas

N.° de celdasDe 4 a 8 millones

Línea de productosGibco

Tipo de productoHepatocitos

Cantidad1 mL

Tipo de célulaCélula de hepatocitos

FormularioCrioconservado

SexoHombre

EspecieRata

Unit Size1 mL

Contenido y almacenamiento

Hepatocitos criopreservados almacenados en nitrógeno líquido (LN2).

Preguntas frecuentes

Who can I call if I have technical questions regarding cryopreserved hepatocytes?

Thermo Fisher Scientific has a dedicated team to answer questions regarding hepatocytes. You can send a question by email (hepaticproducts@thermofisher.com) or contact us by phone: US toll-free: (866) 952 3559

With my hepatocytes, I'm seeing rounding up of the cells, cellular debris, and/or holes in the monolayer, indicating dying cells. What should I do?

This could be due to the toxicity of the test compound. Here are other potential causes and recommendations:

-Sub-optimal culture medium: Use Williams Medium E with Plating and Incubation Supplement Packs; refer to our plating protocol
-Hepatocyte lot not characterized as plateable: Check lot specifications to ensure it is qualified for plating
-Cells were cultured for too long: In general, plateable cryopreserved hepatocytes should not be cultured for more than five days

I'm getting unexpected induction results with my hepatocytes. What could be causing this?

First of all, we recommend comparing results to those reported on our lot-specific characterization specification sheet (human cells) and also referring to our enzyme induction protocol. Here are other potential causes and recommendations:

-Sub-optimal monolayer confluency: Please see our recommendations for 'I have a sub-optimal monolayer confluency for my hepatocytes. What should I do?'
-Poor monolayer integrity: Please see our recommendations for 'With my hepatocytes, I’m seeing rounding up of the cells, cellular debris, and/or holes in the monolayer, indicating dying cells. What should I do?'
-Inappropriate positive control: Check positive control to ensure suitability
-Incorrect concentration of positive control: Use the correct concentration of positive control

I'm seeing sub-optimal bile canlicular formation with my hepatocytes. What should I do?

Please see the following causes and recommendations:

-Hepatocyte lot not transporter-qualified: Check lot specifications to ensure it is transporter-qualified
-Sub-optimal culture medium: Use Williams Medium E with Plating and Incubation Supplement Packs; refer to our plating protocol
-Not enough time for bile canaliculi to form: In general, at least 4-5 days in culture is required for bile canalicular network formation

I'm getting a loss of membrane integrity or cuboidal cell shape with my hepatocytes. What should I do?

Please see the following causes and recommendations:

-Hepatocyte lot not characterized as plateable: Check lot specifications to ensure it is qualified for plating
-Sub-optimal culture medium: Use Williams Medium E with Plating and Incubation Supplement Packs; refer to our plating protocol
-Cells were cultured for too long: In general, plateable cryopreserved hepatocytes should not be cultured for more than five days

View all Rat (Wistar) Cryopreserved Hepatocytes, Plateable Male FAQs