Zenon™ LysoLight™ Green IgG Labeling Reagents

Streamline antibody-drug conjugate screening with the use of Invitrogen Zenon LysoLight Green IgG labeling reagents for the monitoring of antibody internalization. These reagents offer rapid, scalable antibody labeling and a bright fluorescence-based indication of antibody uptake and lysosomal trafficking in target cells when analyzed by flow cytometry, fluorescence microscopy, or high-content screening.

Features of Zenon LysoLight Green IgG labeling reagents include:

• Simplicity—no reagent purification is required
• Accuracy—no wash steps or quenchers are needed, minimizing cross-reactivity or background
• Speed—labeled antibodies are typically ready to use in 10 minutes
• Specificity—dyes are designed for the specific detection of lysosomal trafficking

Zenon LysoLight Green-labeled Fab fragments (i.e., labeling reagent) are directed against the Fc portion of an intact IgG primary antibody to form a labeling complex. Formation of the Fab–antibody complex occurs in fewer than five minutes. Because the LysoLight dye fluorescence dramatically increases upon lysosomal catabolism, the antibodies coupled with the Zenon LysoLight Green IgG labeling reagent provide an excellent measure of lysosomal delivery of internalized antibodies.

Zenon LysoLight Green IgG labeling reagents contain sufficient material to label up to sixteen 96-well plates, depending on the product size and Zenon labeling reagent concentration used. Only a small amount of starting material is needed, and the method is optimized for efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions.

Preserve primary antibody function and affinities

Reactive dye labeling of primary antibodies can have unpredictable and undesirable outcomes. One of these is reduced binding affinity due to label addition in the binding pocket. The Zenon antibody labeling approach, which is targeted to the Fc tail, resolves this potential issue.

Try custom antibody conjugates

If the antibody conjugate you need is not available as one of our stocked products, we will prepare a custom antibody conjugate for you. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 13485:2000 certified.