ZOOM™ Equilibration Trays

Las bandejas de equilibrio ZOOM™ son bandejas prácticas y desechables diseñadas para su uso con casetes ZOOM™ IPGRunner™ para equilibrarMás información

Número de catálogo	Cantidad
ZM0007	10/paq.

Número de catálogo ZM0007

Precio (CLP)

Cantidad:

10/paq.

Las bandejas de equilibrio ZOOM™ son bandejas prácticas y desechables diseñadas para su uso con casetes ZOOM™ IPGRunner™ para equilibrar las tiras ZOOM™ después del isoelectroenfoque de primera dimensión y antes de la SDS-PAGE de segunda dimensión (2D) (Gráfico 1).

Las bandejas de equilibrio ZOOM™ simplifican el proceso de equilibrado e incubación en electroforesis 2D y ofrecen las siguientes ventajas:

• Ahorro de tiempo y mano de obra
• Equilibrio óptimo de las muestras
• Riesgo mínimo de contaminación de las muestras y daños a las tiras IPG

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Línea de productosZOOM

Tipo de productoBandeja de equilibrado

Cantidad10/paq.

Duración de almacenamiento1 año

Condiciones de envíoTemperatura ambiente

Para utilizar con (equipo)ZOOM IPGRunner Mini

Unit Size10⁄pack

Contenido y almacenamiento

Las bandejas de equilibrio ZOOM™ se suministran en un paquete de 10. El kit combinado de casetes ZOOM™ y bandejas de equilibrio ZOOM™ incluye una caja de 10 casetes y un paquete de 10 bandejas. Almacenar a temperatura ambiente. Se garantiza la estabilidad durante un año si se almacena correctamente.

Preguntas frecuentes

I just set up the ZOOM IPGRunner for IEF but don't see any current running through the system. What is the issue?

A possible reason is poor contact between electrodes or incomplete circuit. Make sure that you have added 600 µL deionized water to the Electrode Wicks and the gel is exposed at the anodic and cathodic ends of the cassette. Check the power supply. Be sure to set the “Load Check” to off to enable the power supply to operate at low current.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What are the components of the ZOOM IPGRunner Mini-Cell?

Here are the components of the ZOOM IPGRunner Mini-Cell:

ZOOM IPGRunner Core
ZOOM IPGRunner Lid
Gel Tension Wedge
Buffer Dam
Mini-Cell Chamber

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

I used the ZOOM IPGRunner system and some of my protein spots are missing or appear as a smear. Can you please offer some suggestions?

Here are the possible causes and solutions:

*Protein degradation: Add protease inhibitors during sample preparation (see manual for details).
*Different subunits: Use denaturing conditions (8 M urea).
*Incomplete equilibration: Perform equilibration as described in the manual. Increase the equilibration time.
*Protein precipitates: Increase solubilization reagents in the rehydration buffer (see manual for details. Use appropriate strips based on the pI of the protein sample.
*Low protein load: Increase the protein load. You can load up to 400 µg of fractionated protein sample per ZOOM Strip. Use an accurate and sensitive protein estimation method.
*Insensitive detection method: Use sensitive detection methods such as silver staining or immunoblotting.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

I used the ZOOM IPGRunner system and got vertical streaking. Can you please offer some suggestions?

Here are the possible causes and solutions:

*Protein has got oxidized: Include DTT in the rehydration buffer and perform the alkylation step.
*Impure solutions: Use ultrapure reagents to prepare the rehydration buffer, equilibration buffer, and buffers for SDS/PAGE.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

I used the ZOOM IPGRunner System and got horizontal streaking. Can you please offer some suggestions?

Here are the possible causes and solutions:

*Impure solutions: Use ultrapure reagents to prepare the rehydration buffer, equilibration buffer, and buffers for SDS/PAGE.
*Air bubble between the strip and 2D gel: Smooth out the air bubbles.
*Poor strip rehydration: Rehydrate the strips in 140 µL rehydrating buffer for 1 hour as described in the manual. Rehydration can be extended to overnight if you use 155 µL rehydrating buffer. Make sure that the rehydration buffer covers the strip completely.
*Protein overload: Decrease the protein concentration or lower the sample volume.
*Protein precipitates: Increase solubilization reagents in the rehydration buffer (see manual). Use appropriate strips based on the pI of the protein sample. Do not add more than 10 µL of your sample to 140 µL of rehydration buffer (see manual).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all ZOOM™ Equilibration Trays FAQs