ZOOM™ Carrier Ampholytes 4-6, 10mL
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Invitrogen™

ZOOM™ Carrier Ampholytes 4-6, 10mL

Los anfolitos portadores ZOOM® son moléculas pequeñas y solubles con grupos de carga positivos y negativos. Se clasifican en funciónMás información
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Número de catálogoCantidad
ZM002510 mL
Número de catálogo ZM0025
Precio (CLP)
-
Cantidad:
10 mL
Los anfolitos portadores ZOOM® son moléculas pequeñas y solubles con grupos de carga positivos y negativos. Se clasifican en función de sus puntos isoeléctricos en un campo eléctrico, estableciendo así un gradiente de pH en solución o contribuyendo al gradiente cuando se utilizan con tiras IPG. Los anfolitos portadores ayudan a estabilizar el gradiente de pH y la corriente en las tiras de IPG y ayudan a la solubilidad de proteínas, lo que da como resultado una resolución reproducible de IEF.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Línea de productosZOOM
Tipo de productoÁnfólito portador
Cantidad10 mL
Duración de almacenamiento1 año
Condiciones de envíoTemperatura ambiente
Intervalo de pHentre 4 y 6
Unit Size10 mL
Contenido y almacenamiento
Los anfolitos portadores ZOOM™ se suministran en botellas de 10 ml. Almacenar a +4°C. Se garantiza la estabilidad durante un año si se almacena correctamente.

Preguntas frecuentes

When running the ZOOM IEF Fractionator, if there is not a solubility issue, will the proteins/peptides in the sample migrate to their expected chambers based on isoelectric point in the absence of carrier ampholytes? Won't they act as ampholytes within each chamber?

Proteins are ampholytes, but in general they are poor carrier ampholytes. The profiles of the fractions are similar, but not identical, to those obtained with ampholytes in the sample.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I heat my protein sample prepared using ZOOM 2D Protein Solubilizer?

We do not recommend heating protein samples containing urea over 37 degrees C as elevated temperatures cause urea to hydrolyze to isocyanate, which modifies proteins by carbamylation.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How do you recommend storing protein samples prepared in the Sample Rehydration buffer?

We recommend storing them at -80 degrees C. We do not recommend storing them at -20 degrees C

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How much ampholyte do you recommend adding to the sample rehydration buffer?

The recommended ampholyte concentration in the sample rehydration buffer is 0.5%.

*If you are loading 5-50 µg of protein (pure protein or crude lysate) per ZOOM strip, use 0.5% ampholytes in the sample rehydration buffer.
*If you are loading >50 µg of protein (crude lysate or fractionated sample) per ZOOM Strip, use 0.5-2% ampholytes in the sample rehydration buffer.

Note: Higher ampholyte concentration requires longer focusing times.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What is the maximum recommended volume of protein sample to be added to the Sample Rehydration buffer?

The maximum volume of the protein sample should at most be 1/6 of the final sample volume that will be added to the strip. A good general volume would be 5-10µL. 140 µL of sample diluted in Sample Rehydration buffer is used to rehydrate each ZOOM Strip for the standard rehydration time of one hour. For overnight rehydration, we recommend using 155 µL of diluted sample.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all ZOOM™ Carrier Ampholytes 4-6, 10mL FAQs