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Invitrogen™
AccuPrime™ Pfx DNA Polymerase
AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis.
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| Catalog Number | No. of Reactions |
|---|---|
| 12344024 | 200 Reactions |
| 12344032 | 1000 Reactions |
Catalog number 12344024
Price (CNY)
4,419.00
Each
No. of Reactions:
200 Reactions
Price (CNY)
4,419.00
Each
AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading (3'→5' exonuclease) activity. Platinum anti-Pfx DNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity (see figure). Thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over Platinum Pfx alone.
Benefits of AccuPrime Pfx DNA Polymerase
- Greater than 26 times higher fidelity than Taq DNA polymerase
- Amplification of fragments up to 12 kb
- Minimized PCR optimization
Applications
- Amplification of DNA from complex genomic, viral, and plasmid templates
- RT-PCR
- High-fidelity PCR
- Hot-start PCR
Notes
- AccuPrime Pfx DNA Polymerase is especially effective where very high levels of fidelity are required.
- The 10X AccuPrime Pfx reaction mix contains MgSO4, dNTPs, and thermostable proteins that enhance specific primer-template hybridization during each PCR cycle.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Fidelity (vs. Taq)26X
Hot StartBuilt-In Hot Start
No. of Reactions200 Reactions
OverhangBlunt
PolymeraseAccuPrime Pfx DNA Polymerase
Quantity200 Reactions
Reaction FormatSeparate Components
Shipping ConditionWet or Dry Ice
Size (Final Product)12 kb or less
Volume100 μL
Detection MethodPrimer-probe
For Use With (Application)Hot-start PCR, High-fidelity PCR
GC-Rich PCR PerformanceLow
Reaction SpeedStandard
Unit SizeEach
Contents & Storage
• AccuPrime Pfx DNA Polymerase (1 x 100 μL at 2.5 U/μL)
• 10X AccuPrime Pfx Reaction Mix (1 x 1 mL)
• 50 mM MgSO4 (1 x 1 mL)
Store at -20°C.
• 10X AccuPrime Pfx Reaction Mix (1 x 1 mL)
• 50 mM MgSO4 (1 x 1 mL)
Store at -20°C.