Zitierungen und Referenzen (5)

Gibco™

Streptomyzin-Sulfat

Streptomycinsulfat ist ein wasserlösliches Antibiotikum, das ursprünglich aus dem Bakterium Streptomyces griseus extrahiert wurde. Streptomycinsulfat wirkt, indem es sich anWeitere Informationen

Katalognummer	Menge
11860038	100 g

Katalognummer 11860038

Preis (EUR)

154,00

Menge:

100 g

Preis (EUR)

154,00

Streptomycinsulfat ist ein wasserlösliches Antibiotikum, das ursprünglich aus dem Bakterium Streptomyces griseus extrahiert wurde. Streptomycinsulfat wirkt, indem es sich an die 30S-Untereinheit des bakteriellen Ribosoms anlagert, was zu einer Hemmung der Proteinsynthese und anschließendem Absterben anfälliger Bakterien führt. Streptomycin-Sulfat ist hochwirksam gegen gramnegative Bakterien und hat eine gewisse Aktivität gegen grampositive Bakterien. Gibco™ Streptomycinsulfat wird allein oder in Kombination mit Penicillin, einem hochaktiven Antibiotikum gegen grampositive Bakterien, zur Verhinderung bakterieller Kontamination von Zellkulturen eingesetzt. Der empfohlene Konzentrationsbereich liegt zwischen 50 und 100 µg/ml. Wir bieten eine Vielzahl von Antibiotika und Antimykotika für Zellkulturanwendungen an.

Verwendung des Produkts
Nur für Forschungszwecke: Nicht für diagnostische oder therapeutische Zwecke bei Tieren und Menschen vorgesehen.

cGMP-konforme Fertigung an zwei Standorten
Zur Sicherung der Lieferkette stellen wir Gibco™ Streptomyzinsulfat an zwei gesonderten Standorten in Grand Island, NY (USA), und Schottland (Großbritannien) her. Beide Standorte erfüllen die cGMP-Fertigungsanforderungen und sind ISO 13485-zertifiziert und bei der FDA als Hersteller medizinischer Geräte registriert.

Nur für Forschungszwecke: Nicht für diagnostische oder therapeutische Zwecke an Tieren und Menschen geeignet.

Specifications

Konzentration50 bis 100 μg/ml

KulturtypSäugetierzellkultur, Insektenzellkultur

Zur Verwendung mit (Anwendung)Auswahl der Bakterien

Menge100 g

Haltbarkeit24 Monate

VersandbedingungRaumtemperatur

FormPulver

ProdukttypAntibiotikum

Unit SizeEach

Inhalt und Lagerung

Lagerbedingungen: 2 bis 8 °C
Versandbedingungen: Haltbarkeit bei
Umgebungstemperatur: 24 Monate ab Herstellungsdatum

Häufig gestellte Fragen (FAQ)

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Zitierungen und Referenzen (5)

Zitierungen und Referenzen

Abstract

The cyclin-dependent kinase inhibitor p21WAF1/Cip1 is an antiestrogen-regulated inhibitor of Cdk4 in human breast cancer cells.

Authors: Skildum Andrew J; Mukherjee Shibani; Conrad Susan E;

Journal:J Biol Chem

PubMed ID:11741909

'The MCF-7 cell line is a model of estrogen-dependent, antiestrogen-sensitive human breast cancer. Antiestrogen treatment of MCF-7 cells causes dramatic decreases in both Cdk4 and Cdk2 activities, which leads to a G(1) phase cell cycle arrest. In this report, we investigate the mechanism(s) by which Cdk4 activity is regulated in ... More

Subretinal transplantation of genetically modified human cell lines attenuates loss of visual function in dystrophic rats.

Authors: Lund R D; Adamson P; SauvÃ© Y; Keegan D J; Girman S V; Wang S; Winton H; Kanuga N; Kwan A S; BeauchÃ¨ne L; Zerbib A; Hetherington L; Couraud P O; Coffey P; Greenwood J;

Journal:Proc Natl Acad Sci U S A

PubMed ID:11504951

'Royal College of Surgeons rats are genetically predisposed to undergo significant visual loss caused by a primary dysfunction of retinal pigment epithelial (RPE) cells. By using this model, we have examined the efficacy of subretinal transplantation of two independent human RPE cell lines each exhibiting genetic modifications that confer long-term ... More

Exercise-induced a-ketoglutaric acid stimulates muscle hypertrophy and fat loss through OXGR1-dependent adrenal activation.

Authors:Yuan Y, Xu P, Jiang Q, Cai X, Wang T, Peng W, Sun J, Zhu C, Zhang C, Yue D, He Z, Yang J, Zeng Y, Du M, Zhang F, Ibrahimi L, Schaul S, Jiang Y, Wang J, Sun J, Wang Q, Liu L, Wang S, Wang L, Zhu X, Gao P, Xi Q, Yin C, Li F, Xu G, Zhang Y, Shu G

Journal:EMBO J

PubMed ID:32104923

'Beneficial effects of resistance exercise on metabolic health and particularly muscle hypertrophy and fat loss are well established, but the underlying chemical and physiological mechanisms are not fully understood. Here, we identified a myometabolite-mediated metabolic pathway that is essential for the beneficial metabolic effects of resistance exercise in mice. We ... More

Human GBP1 is a microbe-specific gatekeeper of macrophage apoptosis and pyroptosis.

Authors:Fisch D, Bando H, Clough B, Hornung V, Yamamoto M, Shenoy AR, Frickel EM

Journal:EMBO J

PubMed ID:31268602

'The guanylate binding protein (GBP) family of interferon-inducible GTPases promotes antimicrobial immunity and cell death. During bacterial infection, multiple mouse Gbps, human GBP2, and GBP5 support the activation of caspase-1-containing inflammasome complexes or caspase-4 which trigger pyroptosis. Whether GBPs regulate other forms of cell death is not known. The apicomplexan ... More

Mycobacterial Mutagenesis and Drug Resistance Are Controlled by Phosphorylation- and Cardiolipin-Mediated Inhibition of the RecA Coprotease.

Authors:Wipperman MF, Heaton BE, Nautiyal A, Adefisayo O, Evans H, Gupta R, van Ditmarsch D, Soni R, Hendrickson R, Johnson J, Krogan N, Glickman MS

Journal:Mol Cell

PubMed ID:30174294

'Infection with Mycobacterium tuberculosis continues to cause substantial human mortality, in part because of the emergence of antimicrobial resistance. Antimicrobial resistance in tuberculosis is solely the result of chromosomal mutations that modify drug activators or targets, yet the mechanisms controlling the mycobacterial DNA-damage response (DDR) remain incompletely defined. Here, we ... More