AmnioMAX™ C-100 Basalmedium
AmnioMAX™ C-100 Basalmedium
AmnioMAX™ C-100 Basalmedium
Gibco™

AmnioMAX™ C-100 Basalmedium

Gibco® AmnioMAX™ C-100 Basal Medium is part of a kit used to prepare a fully-supplemented Gibco® AmnioMAX™ C-100 Complete MediumWeitere Informationen
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KatalognummerMenge
17001074450 mL
1700108290 mL
Katalognummer 17001074
Preis (EUR)
307,00
Each
Menge:
450 mL
Preis (EUR)
307,00
Each

Gibco® AmnioMAX™ C-100 Basal Medium is part of a kit used to prepare a fully-supplemented Gibco® AmnioMAX™ C-100 Complete Medium developed for the short term culture of human amniotic fluid cells for cytogenetic studies and in vitro diagnostic procedures.

Gibco® AmnioMAX™ C-100 Complete Medium features:

  • Easy-to-use format
  • Quality and performance testing
  • Unique, optimized formulation

Easy-to-use format

Gibco® AmnioMAX™ C-100 Complete Medium is a two-part kit for preparation of a complete medium that requires no additional supplementation. The complete medium can be stored at 2–8°C for up to ten days.

Quality and performance testing

Every lot of Gibco® AmnioMAX™ C-100 Complete Medium is performance tested by a certified US reference cytogenetics laboratory to ensure consistently superior performance. Pooled primary human amniotic fluid samples are cultured for six days in Gibco® AmnioMAX™ C-100 Complete Medium before measuring the total number of colonies and total number of mitotic colonies. In addition, each lot is tested for sterility, pH and osmolality.

Unique, optimized formulation

Gibco® AmnioMAX™ C-100 Complete Medium contains Fetal Bovine Serum (FBS), gentamicin, and L-glutamine to maximize cell attachment and growth. This optimized medium also has an enhanced buffering system that provides greater pH stability during culture manipulations.

cGMP Manufacturing and Quality System

Gibco® AmnioMAX™ C-100 Basal Medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

In-vitro-Diagnostik
Specifications
ZelltypAmniozyten
ProduktlinieAmnioMAX
ProdukttypBasalmedium für Zellen der Amnionflüssigkeit
Menge450 mL
Haltbarkeit16 Monate
VersandbedingungRaumtemperatur
SpeziesHuman
FormFlüssig
Serum LevelStandard-Serumzugabe
SterilitätSteril gefiltert
Sterilization MethodSterile-filtered
Mit AdditivenGlutamin, HEPES
Unit SizeEach
Inhalt und Lagerung
Lagerbedingungen: 2 bis 8 °C
Versandbedingungen: Haltbarkeit bei
Umgebungstemperatur: 16 Monate ab Herstellungsdatum. Das vollständige Medium innerhalb von 7 bis 10 Tagen nach dem Öffnen verwenden.

Zitierungen und Referenzen (5)

Zitierungen und Referenzen
Abstract
Induced pluripotent stem cells offer new approach to therapy in thalassemia and sickle cell anemia and option in prenatal diagnosis in genetic diseases.
Authors:Ye L, Chang JC, Lin C, Sun X, Yu J, Kan YW,
Journal:Proc Natl Acad Sci U S A
PubMed ID:19482945
'The innovation of reprogramming somatic cells to induced pluripotent stem cells provides a possible new approach to treat beta-thalassemia and other genetic diseases such as sickle cell anemia. Induced pluripotent stem (iPS) cells can be made from these patients'' somatic cells and the mutation in the beta-globin gene corrected by ... More
Reactivation of XIST in normal fibroblasts and a somatic cell hybrid: abnormal localization of XIST RNA in hybrid cells.
Authors:Hansen RS, Canfield TK, Stanek AM, Keitges EA, Gartler SM,
Journal:Proc Natl Acad Sci U S A
PubMed ID:9560241
'The XIST gene, expressed only from the inactive X chromosome, is a critical component of X inactivation. Although apparently unnecessary for maintenance of inactivation, XIST expression is thought to be sufficient for inactivation of genes in cis even when XIST is located abnormally on another chromosome. This repression appears to ... More
Specific tumour-associated methylation in normal human term placenta and first-trimester cytotrophoblasts.
Authors:Novakovic B, Rakyan V, Ng HK, Manuelpillai U, Dewi C, Wong NC, Morley R, Down T, Beck S, Craig JM, Saffery R,
Journal:Mol Hum Reprod
PubMed ID:18708652
'Human placentation displays many similarities with tumourigenesis, including rapid cell division, migration and invasion, overlapping gene expression profiles and escape from immune detection. Recent data have identified promoter methylation in the Ras association factor and adenomatous polyposis coli tumour suppressor genes as part of this process. However, the extent of ... More
Placenta-specific methylation of the vitamin D 24-hydroxylase gene: implications for feedback autoregulation of active vitamin D levels at the fetomaternal interface.
Authors:Novakovic B, Sibson M, Ng HK, Manuelpillai U, Rakyan V, Down T, Beck S, Fournier T, Evain-Brion D, Dimitriadis E, Craig JM, Morley R, Saffery R,
Journal:J Biol Chem
PubMed ID:19237542
'Plasma concentrations of biologically active vitamin D (1,25-(OH)(2)D) are tightly controlled via feedback regulation of renal 1alpha-hydroxylase (CYP27B1; positive) and 24-hydroxylase (CYP24A1; catabolic) enzymes. In pregnancy, this regulation is uncoupled, and 1,25-(OH)(2)D levels are significantly elevated, suggesting a role in pregnancy progression. Epigenetic regulation of CYP27B1 and CYP24A1 has previously ... More
Development of transgenic cloned pig models of skin inflammation by DNA transposon-directed ectopic expression of human ß1 and a2 integrin.
Authors:Staunstrup NH, Madsen J, Primo MN, Li J, Liu Y, Kragh PM, Li R, Schmidt M, Purup S, Dagnæs-Hansen F, Svensson L, Petersen TK, Callesen H, Bolund L, Mikkelsen JG,
Journal:PLoS One
PubMed ID:22590584
Integrins constitute a superfamily of transmembrane signaling receptors that play pivotal roles in cutaneous homeostasis by modulating cell growth and differentiation as well as inflammatory responses in the skin. Subrabasal expression of integrins a2 and/or ß1 entails hyperproliferation and aberrant differentiation of keratinocytes and leads to dermal and epidermal influx ... More