GlycanPac™ AXH-1 HPLC Columns

Use Thermo Scientific™ GlycanPac™ AXH-1 LC Columns for qualitative, quantitative, and structural characterization of uncharged and charged glycans present in biological molecules. This high-performance, silica-based high-performance liquid chromatography (HPLC) column provides simultaneous separation of glycans by charge, size and polarity. Achieve high-resolution and high-throughput analysis with unique selectivity for biologically important glycans, either labeled or native, by LC-fluorescence and LC-mass spectrometry (MS) methods.

The GlycanPac AXH-1 LC column, based on innovative mixed-mode surface chemistry, combines both weak anion-exchange (WAX) and hydrophilic interaction (HILIC) retention mechanisms for optimal selectivity and resolution power. The WAX functionality provides retention and selectivity for negatively charged glycans while the HILIC mode facilitates the separation of glycans of the same charge according to their polarity and size. As a result, the GlycanPac AXH-1 LC column provides unparalleled capabilities for glycan separations.

Unique Glycan Selectivity Based on Charge, Size and Polarity

• Excellent resolution for both native and labeled glycans
• Useful for both high-resolution glycan profile characterization and easy quantification of glycans based on charge
• Compatible with fluorescence and MS detection methods
• High chromatographic efficiency and excellent column stability

GlycanPac AXH-1 LC columns are based on high-purity and spherical silica substrates in both 1.9 and 3μm sizes for UHPLC and standard HPLC applications, respectively. The various dimensions of GlycanPac AXH-1 LC columns meet the needs of your applications with different resolutions, throughput and instrument requirements.