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Citations & References (90)
Invitrogen™
PLUS™ Reagent
PLUS™ Reagent is used in conjunction with various transfection reagents, such as Lipofectamine™ Reagent, to improve transfection efficiency in adherentRead more
| Catalog Number | Quantity |
|---|---|
| 11514015 | 0.85 mL |
Catalog number 11514015
Price (EUR)
689,65
Online Exclusive
718,00Save 28,35 (4%)
0.85 mL
Quantity:
0.85 mL
Price (EUR)
689,65
Online Exclusive
718,00Save 28,35 (4%)
0.85 mL
PLUS™ Reagent is used in conjunction with various transfection reagents, such as Lipofectamine™ Reagent, to improve transfection efficiency in adherent cell lines.
Using PLUS™ Reagent
Some customers find that when using PLUS™ Reagent, less optimization of DNA concentration is needed to achieve high transfection performance. The addition of PLUS™ Reagent may also reduce the quantities of DNA and lipid required by approximately one-half compared to lipid alone. PLUS™ Reagent was specifically designed to expand the array of transfectable cell types. When used with Lipofectamine™ Reagent, PLUS™ Reagent has been demonstrated to improve the transfection of BHK-21, NIH 3T3, and other cell lines.
Using PLUS™ Reagent
Some customers find that when using PLUS™ Reagent, less optimization of DNA concentration is needed to achieve high transfection performance. The addition of PLUS™ Reagent may also reduce the quantities of DNA and lipid required by approximately one-half compared to lipid alone. PLUS™ Reagent was specifically designed to expand the array of transfectable cell types. When used with Lipofectamine™ Reagent, PLUS™ Reagent has been demonstrated to improve the transfection of BHK-21, NIH 3T3, and other cell lines.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)Transfection
High-throughput CompatibilityNot High-throughput Compatible (Manual)
Product LinePLUS
Product TypeReagent
Quantity0.85 mL
Serum CompatibleYes
Shipping ConditionWet Ice
Cell TypeEstablished Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells
Format6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Sample TypePlasmid DNA, RNAi Plasmids (shRNA, miR)
Transfection TechniqueLipid-based Transfection
Unit Size0.85 mL
Contents & Storage
Contains one vial (0.85 ml) PLUS™ Reagent. Store at 4°C. Do not freeze.
Frequently asked questions (FAQs)
I accidentally left my lipid reagent at room temperature. Can I still use it?
Do you offer a reagent for the transfection of endothelial cells?
Can I use the PLUS Reagent with Lipofectamine RNAiMAX?
Why do I have to dilute my DNA in medium before adding the PLUS Reagent? Can I just add them together?
What is the PLUS Reagent?
Citations & References (90)
Citations & References
Abstract
c-Jun is a JNK-independent coactivator of the PU.1 transcription factor.
Journal:J Biol Chem
PubMed ID:9988737
The ETS domain transcription factor PU.1 is necessary for the development of monocytes and regulates, in particular, the expression of the monocyte-specific macrophage colony-stimulating factor (M-CSF) receptor, which is critical for monocytic cell survival, proliferation, and differentiation. The bZIP transcription factor c-Jun, which is part of the AP-1 transcription factor
Receptor-selective effects of endogenous RGS3 and RGS5 to regulate mitogen-activated protein kinase activation in rat vascular smooth muscle cells.
Journal:J Biol Chem
PubMed ID:12006602
Regulators of G protein signaling (RGS) proteins compose a highly diverse protein family best known for inhibition of G protein signaling by enhancing GTP hydrolysis by Galpha subunits. Little is known about the function of endogenous RGS proteins. In this study, we used synthetic ribozymes targeted to RGS2, RGS3, RGS5,
A
Journal:J Biol Chem
PubMed ID:11973330
The large size (six membrane-spanning repeats in each of four domains) and asymmetric architecture of the voltage-dependent Na+ channel has hindered determination of its structure. With the goal of determining the minimum structure of the Na+ channel permeation pathway, we created two stable cell lines expressing the voltage-dependent rat skeletal
Vesicle-associated membrane protein-2/synaptobrevin binding to synaptotagmin I promotes O-glycosylation of synaptotagmin I.
Journal:J Biol Chem
PubMed ID:12048209
Synaptotagmin I (Syt I), an evolutionarily conserved integral membrane protein of synaptic vesicles, is now known to regulate Ca2+-dependent neurotransmitter release. Syt I protein should undergo several post-translational modifications before maturation and subsequent functioning on synaptic vesicles (e.g. N-glycosylation and fatty acylation in vertebrate Syt I), because the apparent molecular
Extracellular Export of Sphingosine Kinase-1 Enzyme. SPHINGOSINE 1-PHOSPHATE GENERATION AND THE INDUCTION OF ANGIOGENIC VASCULAR MATURATION.
Journal:J Biol Chem
PubMed ID:11741921
The enzyme sphingosine kinase (SK) catalyzes the formation of sphingosine 1-phosphate (S1P), a bioactive lipid that acts extracellularly on G protein-coupled receptors of the S1P(1)/EDG-1 subfamily. Although S1P is formed in the cytosol of various cells, S1P release is not understood and is controversial because this lipid mediator is also