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Gibco™

Grace's Insect Medium, supplemented

Grace's Insect Medium, Supplemented, was originally designed for the growth of cells from the Australian Emperor Gum Moth, Antherea eucalypti.Read more
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Catalog NumberQuantity
11605045
also known as 11605-045
500 mL
11605086
also known as 11605-086
10 x 500 mL
This chemical may require us to obtain additional information for our regulatory and chemical compliance records. If required, we will contact you for this information once your order is placed.
Catalog number 11605045
also known as 11605-045
Price (EUR)
81,65
キャンペーン価格
95,00
Save 13,35 (14%)
Each
Quantity:
500 mL
Customize this product
Price (EUR)
81,65
キャンペーン価格
95,00
Save 13,35 (14%)
Each
Grace's Insect Medium, Supplemented, was originally designed for the growth of cells from the Australian Emperor Gum Moth, Antherea eucalypti. Grace's Supplemented Insect Medium is now widely used for the growth of Spodeptera frugiperda cells, Sf9 and Sf21.

Sf9 and Sf21 cells are routinely used to express recombinant protein using the Baculovirus Expression Vector System (BEVS). We offer a variety of Gibco™ media options for a range of insect cell culture applications.

Formulation
This Grace's Insect Medium contains lactalbumin hydrolysate, yeastolate, L-glutamine, and sodium bicarbonate. The complete formulation is available. Grace's Supplemented Insect Medium contains lactalbumin hydrolysate and yeastolate not commonly found in the unsupplemented version.

Dual site cGMP manufacturing and quality system
Gibco™ Grace's Supplemented Insect Medium is manufactured at a cGMP-compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco™ Grace's Supplemented Insect Medium made in our Grand Island facility (11605-094). This facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and 9001 standards.

Grace's Supplemented Insect Medium contains lactalbumin hydrolysate and yeastolate, and is commonly supplemented with 10% Fetal Bovine Serum (FBS) before use. Grace's Supplemented Insect Medium requires ambient CO2 in order to maintain physiological pH.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell LineSf21, Sf9
Cell TypeInsect Cell
Inorganic SaltsCalcium, Magnesium
Product LineGibco
Product TypeGrace's Insect Medium
Quantity500 mL
Shipping ConditionRoom Temperature
Culture TypeInsect Cell Culture
FormLiquid
With AdditivesGlutamine, Lactalbumin Hydrolysate, Yeastolate, Sodium Bicarbonate
Without AdditivesNo Insect Hemolymph
Unit SizeEach
Contents & Storage
Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Frequently asked questions (FAQs)

For how long can I store Grace's Insect Medium, unsupplemented at room temperature?

We ship this product under “ambient” conditions throughout the year. We have performed shipping studies that shown components to be stable for at least 7 days at ambient temperatures. The important factor is to ensure that the media is not exposed to light at the warmer temperatures as there are vitamins and amino acids that will breakdown with light exposure.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What additional supplements are required in Grace's Insect Cell Culture Medium, Unsupplemented to make a "complete" formulation that will support insect cell growth?

Grace's Insect Cell Culture Medium, Unsupplemented requires addition of 3.33 g/L of lactalbumin hydrolysate and 3.33 g/L of yeastolate for the “base” growth medium. For the “complete” growth medium that will support insect cell growth, 10% fetal bovine serum must also be added. This “complete” growth medium is also referred to as TNM-FH.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.