Gateway™ pDEST™8 Vector

Citations & References (5)

Invitrogen™

Gateway™ pDEST™8 Vector

To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast,Read more

Catalog Number	Quantity
11804010 also known as 11804-010	6 μg

Catalog number 11804010

also known as 11804-010

Price (EUR)

1.446,00

Add to cart

Quantity:

Price (EUR)

1.446,00

Add to cart

To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway™ destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available.

Additional materials required, available separately: Gateway™ entry clone, Gateway™ LR Clonase™ enzyme mix, and reaction buffer.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product TypeExpression Vector

Quantity6 μg

VectorpDEST, Gateway Baculovirus Vectors

Cloning MethodGateway

Product LineGateway

PromoterPolyhedrin

Protein TagUntagged

Unit SizeEach

Contents & Storage

All destination vectors are provided lyophilized and supercoiled.

Frequently asked questions (FAQs)

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all Gateway™ pDEST™8 Vector FAQs

Citations & References (5)

Citations & References

Abstract

Target-induced formation of neuraminidase inhibitors from in vitro virtual combinatorial libraries.

Authors: HochgÃ¼rtel Matthias; Kroth Heiko; Piecha Dorothea; Hofmann Michael W; Nicolau Claude; Krause Sonja; Schaaf Otmar; Sonnenmoser Gabriele; Eliseev Alexey V;

Journal:Proc Natl Acad Sci U S A

PubMed ID:11891312

'Neuraminidase, a key enzyme responsible for influenza virus propagation, has been used as a template for selective synthesis of small subsets of its own inhibitors from theoretically highly diverse dynamic combinatorial libraries. We show that the library building blocks, aldehydes and amines, form significant amounts of the library components resulting ... More

Enhanced production of green fluorescent fusion proteins in a baculovirus expression system by addition of secretion signal.

Authors:Katagiri Y, Ingham KC.

Journal:Biotechniques

PubMed ID:12139250

All Six Modules of the Gelatin-binding Domain of Fibronectin Are Required for Full Affinity.

Authors:Katagiri Y, Brew SA, Ingham KC,

Journal:J Biol Chem

PubMed ID:12538576

The gelatin-binding sites of fibronectin are confined to a 42-kDa region having four type I and two type II modules in the following order: I(6)-II(1)-II(2)-I(7)-I(8)-I(9). To determine the relative importance of each module for recognition of gelatin, recombinant green fluorescent fusion proteins were prepared in which individual modules or groups ... More

Cleavage of von Willebrand Factor Requires the Spacer Domain of the Metalloprotease ADAMTS13.

Authors:Zheng X, Nishio K, Majerus EM, Sadler JE,

Journal:J Biol Chem

PubMed ID:12791682

ADAMTS13 consists of a reprolysin-type metalloprotease domain followed by a disintegrin domain, a thrombospondin type 1 motif (TSP1), Cys-rich and spacer domains, seven more TSP1 motifs, and two CUB domains. ADAMTS13 limits platelet accumulation in microvascular thrombi by cleaving the Tyr1605-Met1606 bond in von Willebrand factor, and ADAMTS13 deficiency causes ... More

DNA cloning using in vitro site-specific recombination.

Authors: Hartley J L; Temple G F; Brasch M A;

Journal:Genome Res

PubMed ID:11076863

As a result of numerous genome sequencing projects, large numbers of candidate open reading frames are being identified, many of which have no known function. Analysis of these genes typically involves the transfer of DNA segments into a variety of vector backgrounds for protein expression and functional analysis. We describe ... More