RPMI 1640 Medium, no phenol red

Citations & References (3)

Gibco™

RPMI 1640 Medium, no phenol red

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park MemorialRead more

Catalog Number	Quantity
11835030	500 mL

Catalog number 11835030

Price (EUR)

46,03

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Quantity:

500 mL

Customize this product

Price (EUR)

46,03

Add to cart

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI is modified as follows:

With	Without
• L-glutamine	• HEPES
	• Phenol Red

The complete formulation is available.

Using RPMI
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B₁₂, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

Specifications

Cell LineHeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas

Cell TypeLeukemic Cells

Concentration1 X

Manufacturing QualitycGMP-compliant under the ISO 13485 standard

Product LineGibco

Product TypeRPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)

Quantity500 mL

Shelf Life12 Months From Date of Manufacture

Shipping ConditionRoom Temperature

ClassificationAnimal Origin-free

FormLiquid

Serum LevelStandard Serum Supplementation

SterilitySterile-filtered

Sterilization MethodSterile-filtered

With AdditivesGlutamine

Without AdditivesNo HEPES, No Phenol Red, No Sodium Pyruvate

Unit SizeEach

Contents & Storage

Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Frequently asked questions (FAQs)

How light sensitive is RPMI 1640 media? Should I also be protecting it from LED light?

While we know that different wavelengths of light are worse than others for exposure, we would recommend as a best practice to protect the medium from all forms of light exposure including LEDs, as much as possible to ensure optimal performance, as several components within the medium are light sensitive, such as vitamins.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the density (g/L) for RPMI 1640 Medium?

We have specific gravity information for RPMI 1640 Medium: 1.006 kg/L. In this case, the specific gravity is the same as density as the solvent is water.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer RPMI 1640 medium without both methionine and phenol red?

We offer RPMI 1640 Medium, no methionine (Cat. No. A1451701) and also offer RPMI Medium, no phenol red (Cat. Nos. 11835030, 11835055, 11835063, and 11835105). However, we do not offer RPMI 1640 medium without both methionine and phenol red as a standard catalog product. Our Customs group can make it for you as a custom media. If you are interested, please complete this Custom order inquiry form PDF (https://www.thermofisher.com/content/dam/LifeTech/global/applied-sciences/pdfs/Bioproduction/Gibco-Custom-Media-Buffers-Request-Form.pdf) and email it to custommedia@thermofisher.com. Our Customs group will review your requirements and get back to you within a couple of days with pricing, ordering information, and lead time. After that, you have the choice of proceeding with an “official” order or declining.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I understand that some media are worse than others for fluorescence imaging. How do I choose?

Most media contain phenol red, which can quench fluorescent dyes in the visible wavelengths. Most media also contain autofluorescent components, such as riboflavin, which can reduce signal-to-background. We offer FluoroBrite DMEM and HEPES-based Live Cell Imaging Solution, which have been optimized for fluorescent imaging. We also offer a number of media without phenol red. But if none of these are reasonable options for your experiment, then we also offer BackDrop Background Suppressor ReadyProbes Reagent, which can be added to quench media autofluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Should I be concerned about phenol red in my media when labeling my live cells with fluorescent dyes?

Some cell types accumulate phenol red, and this can pose a problem in the use of many fluorescent probes. Phenol red can quench visible-wavelength dyes and, although phenol red is non-fluorescent, various impurities may be fluorescent. We have many phenol red-free media to choose from. Our Live Cell Imaging Solution (HEPES-based) and our FluoroBrite DMEM have been optimized to be phenol red-free as well as to be non-autofluorescent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all RPMI 1640 Medium, no phenol red, 500 mL FAQs

Citations & References (3)

Citations & References

Abstract

Inhibition of both paracrine and autocrine VEGF/ VEGFR-2 signaling pathways is essential to induce long-term remission of xenotransplanted human leukemias.

Authors: Dias S; Hattori K; Heissig B; Zhu Z; Wu Y; Witte L; Hicklin D J; Tateno M; Bohlen P; Moore M A; Rafii S;

Journal:Proc Natl Acad Sci U S A

PubMed ID:11553814

'Antiangiogenic agents block the effects of tumor-derived angiogenic factors (paracrine factors), such as vascular endothelial growth factor (VEGF), on endothelial cells (EC), inhibiting the growth of solid tumors. However, whether inhibition of angiogenesis also may play a role in liquid tumors is not well established. We recently have shown that ... More

Regulation of neural differentiation by normal and mutant (G654A, amyloidogenic) gelsolin.

Authors: Westberg J A; Zhang K Z; Andersson L C;

Journal:FASEB J

PubMed ID:10463954

'Gelsolin belongs to a family of proteins that modulate the structural dynamics of cytoskeletal actin. Gelsolin activity is required for the redistribution of actin occurring during membrane ruffling, cell crawling, and platelet activation. A point mutation (G654A) in the gelsolin gene causes a dominantly inherited systemic amyloidosis called familial amyloidosis ... More

Inhibition of mitochondrial respiration by endogenous nitric oxide: a critical step in Fas signaling.

Authors: BeltrÃ¡n BelÃ©n; Quintero Marisol; GarcÃa-ZaragozÃ¡ Eugenia; O'Connor Enrique; Esplugues Juan V; Moncada Salvador;

Journal:Proc Natl Acad Sci U S A

PubMed ID:12077295

We have found that activation of human adult T cell leukemia (Jurkat) cells with anti-Fas Ab leads, in a concentration-dependent manner, to an early burst of production of nitric oxide (NO), which inhibits cell respiration. This results in mitochondrial hyperpolarization, dependent on the hydrolysis of glycolytic ATP by the F1F(o)-ATPase ... More