Search
Citations & References (3)
Invitrogen™
Gateway™ pcDNA™-DEST47 Vector
The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The Gateway™ pcDNA™-DEST47 vectorRead more
| Catalog Number | Quantity |
|---|---|
| 12281010 | 6 μg |
Catalog number 12281010
Price (EUR)
1.292,00
Each
Quantity:
6 μg
Price (EUR)
1.292,00
Each
The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The Gateway™ pcDNA™-DEST47 vector offers the following key features:
•C-terminal Cycle 3 GFP tag for rapid detection of recombinant protein
•Cytomegalovirus (CMV) promoter for high-level expression
•attR sites for Gateway™ cloning, enabling recombination with attL-flanked fragments
•Neomycin resistance gene for stable selection
•Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli
Gateway™ Cloning
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway™ destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ ORF Clone. The following table lists a variety of available destination vectors.
Additional materials required, available separately: Gateway™ entry clone, appropriate Gateway™ LR Clonase™ enzyme mix, and reaction buffer.
•C-terminal Cycle 3 GFP tag for rapid detection of recombinant protein
•Cytomegalovirus (CMV) promoter for high-level expression
•attR sites for Gateway™ cloning, enabling recombination with attL-flanked fragments
•Neomycin resistance gene for stable selection
•Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli
Gateway™ Cloning
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway™ destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ ORF Clone. The following table lists a variety of available destination vectors.
Additional materials required, available separately: Gateway™ entry clone, appropriate Gateway™ LR Clonase™ enzyme mix, and reaction buffer.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Constitutive or Inducible SystemConstitutive
Delivery TypeTransfection
For Use With (Application)Constitutive Expression
Product TypeGateway System Destination Expression Vector
Quantity6 μg
Reporter GeneGFP (Cycle 3)
Selection Agent (Eukaryotic)Geneticin™ (G-418)
VectorpDEST, pcDNA
Cloning MethodGateway
Product LineGateway, pDEST
PromoterCMV
Protein TagGFP (Cycle 3)
Unit SizeEach
Contents & Storage
All destination vectors are provided lyophilized and supercoiled.
Frequently asked questions (FAQs)
Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?
Do you have a recommended single-step protocol for BP/LR recombination?
How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?
Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?
Do you offer Gateway vectors for expression in plants?
Citations & References (3)
Citations & References
Abstract
MARK4 is a novel microtubule-associated proteins/microtubule affinity-regulating kinase that binds to the cellular microtubule network and to centrosomes.
Journal:J Biol Chem
PubMed ID:14594945
The MARK protein kinases were originally identified by their ability to phosphorylate a serine motif in the microtubule-binding domain of tau that is critical for microtubule binding. Here, we report the cloning and expression of a novel human paralog, MARK4, which shares 75% overall homology with MARK1-3 and is predominantly
Calpain 3 is activated through autolysis within the active site and lyses sarcomeric and sarcolemmal components.
Journal:Mol Cell Biol
PubMed ID:14645524
Calpain 3 (Capn3) is known as the skeletal muscle-specific member of the calpains, a family of intracellular nonlysosomal cysteine proteases. This enigmatic protease has many unique features among the calpain family and, importantly, mutations in Capn3 have been shown to be responsible for limb girdle muscular dystrophy type 2A. Here
Serinc, an activity-regulated protein family, incorporates serine into membrane lipid synthesis.
Journal:J Biol Chem
PubMed ID:16120614
Cell membranes contain various transporter proteins, some of which are responsible for transferring amino acids across membrane. In this study, we report another class of carrier proteins, termed Serinc1-5, that incorporates a polar amino acid serine into membranes and facilitates the synthesis of two serine-derived lipids, phosphatidylserine and sphingolipids. Serinc