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Advanced RPMI 1640 Medium
Citations & References (5)
Gibco™

Advanced RPMI 1640 Medium

Advanced RPMI (Roswell Park Memorial Institute) 1640 is a widely used basal medium that allows the culture of mammalian cellsRead more
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Catalog NumberQuantity
12633012500 mL
1263302010 x 500 mL
Catalog number 12633012
Price (EUR)
43,26
Each
Quantity:
500 mL
Customize this product
Price (EUR)
43,26
Each

Advanced RPMI (Roswell Park Memorial Institute) 1640 is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic RPMI 1640, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology.i Cells successfully cultured in Advanced RPMI 1640, with no adaptation, include Sp2, Vero, Raji, Daudi, and Jurkat.

This Advanced RPMI 1640 is manufactured as follows:

With: glucose, non-essential amino acids, sodium pyruvate, phenol red

Without: L-glutamine, HEPES

The complete formulation is available.

Gibco Advanced RPMI 1640 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.

Advanced RPMI 1640 requires supplementation with 1-5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX supplement. Many cell lines do not require adaptation to this medium. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced RPMI 1640 uses a sodium bicarbonate buffer system (2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

iAdvanced Media: Reduced Sera Solutions. Quest, 2004. 1.3:26-30.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell LineSp2, Vero, Raji, Daudi, and Jurkat
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco
Product TypeRPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Quantity500 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin
FormLiquid
Serum LevelReduced Serum
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesGlucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)
Without AdditivesNo Glutamine, No HEPES
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Frequently asked questions (FAQs)

What is the glucose concentration in Advanced RPMI 1640 Medium (Cat. No. 12633012, 12633020)?

Advanced RPMI 1640 Medium (Cat. No. 12633012, 12633020) contains glucose (D-Glucose or Dextrose) at a concentration of 2 g/L.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer Advanced RPMI 1640 Medium (Cat. No. 12633012 or 12633020) without phenol red?

We do not offer Advanced RPMI 1640 medium without phenol red as a standard catalog product. However, our Customs group can make it for you as a custom media. If you are interested, please complete this Custom order inquiry form PDF (https://www.thermofisher.com/content/dam/LifeTech/global/applied-sciences/pdfs/Bioproduction/Gibco-Custom-Media-Buffers-Request-Form.pdf) and email it to custommedia@thermofisher.com. Our Customs group will review your requirements and get back to you within a couple of days with pricing, ordering information, and lead time. After that, you have the choice of proceeding with an “official” order or declining.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between Advanced RPMI 1640 Medium and other RPMI 1640 formulations?

Advanced Media are widely used basal media, which require 50-90% less Fetal Bovine Serum (FBS) supplementation compared to conventional basal media formulations. The Advanced media is unique due to the addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

View all Advanced RPMI 1640 Medium, 500 mL FAQs

Citations & References (5)

Citations & References
Abstract
Viral complementation allows HIV-1 replication without integration.
Authors:Gelderblom HC,Vatakis DN,Burke SA,Lawrie SD,Bristol GC,Levy DN
Journal:Retrovirology
PubMed ID:18613957
BACKGROUND: The integration of HIV-1 DNA into cellular chromatin is required for high levels of viral gene expression and for the production of new virions. However, the majority of HIV-1 DNA remains unintegrated and is generally considered a replicative dead-end. A limited amount of early gene expression from unintegrated DNA ... More
Modelling kidney disease with CRISPR-mutant kidney organoids derived from human pluripotent epiblast spheroids.
Authors:Freedman BS,Brooks CR,Lam AQ,Fu H,Morizane R,Agrawal V,Saad AF,Li MK,Hughes MR,Werff RV,Peters DT,Lu J,Baccei A,Siedlecki AM,Valerius MT,Musunuru K,McNagny KM,Steinman TI,Zhou J,Lerou PH,Bonventre JV
Journal:Nature communications
PubMed ID:26493500
Human-pluripotent-stem-cell-derived kidney cells (hPSC-KCs) have important potential for disease modelling and regeneration. Whether the hPSC-KCs can reconstitute tissue-specific phenotypes is currently unknown. Here we show that hPSC-KCs self-organize into kidney organoids that functionally recapitulate tissue-specific epithelial physiology, including disease phenotypes after genome editing. In three-dimensional cultures, epiblast-stage hPSCs form spheroids ... More
Drug screening of cancer cell lines and human primary tumors using droplet microfluidics.
Authors:Wong AH,Li H,Jia Y,Mak PI,Martins RPDS,Liu Y,Vong CM,Wong HC,Wong PK,Wang H,Sun H,Deng CX
Journal:Scientific reports
PubMed ID:28831060
Precision Medicine in Oncology requires tailoring of therapeutic strategies to individual cancer patients. Due to the limited quantity of tumor samples, this proves to be difficult, especially for early stage cancer patients whose tumors are small. In this study, we exploited a 2.4 × 2.4 centimeters polydimethylsiloxane (PDMS) based microfluidic chip which ... More
The G-Protein-Coupled Estrogen Receptor Agonist G-1 Inhibits Proliferation and Causes Apoptosis in Leukemia Cell Lines of T Lineage.
Authors:Torres-López L,Olivas-Aguirre M,Villatoro-Gómez K,Dobrovinskaya O
Journal:Frontiers in cell and developmental biology
PubMed ID:35237599
The G-protein–coupled estrogen receptor (GPER) mediates non-genomic action of estrogen. Due to its differential expression in some tumors as compared to the original healthy tissues, the GPER has been proposed as a therapeutic target. Accordingly, the non-steroidal GPER agonist G-1, which has often demonstrated marked cytotoxicity in experimental models, has ... More
Nephron organoids derived from human pluripotent stem cells model kidney development and injury.
Authors:Morizane R,Lam AQ,Freedman BS,Kishi S,Valerius MT,Bonventre JV
Journal:Nature biotechnology
PubMed ID:26458176
Kidney cells and tissues derived from human pluripotent stem cells (hPSCs) would enable organ regeneration, disease modeling, and drug screening in vitro. We established an efficient, chemically defined protocol for differentiating hPSCs into multipotent nephron progenitor cells (NPCs) that can form nephron-like structures. By recapitulating metanephric kidney development in vitro, ... More