FreeStyle™ MAX Reagent
FreeStyle™ MAX Reagent
Actual product may vary
Citations & References (1)
Gibco™

FreeStyle™ MAX Reagent

FreeStyle™ MAX Reagent is a proprietary, animal origin–free formulation for transfecting plasmid DNA into eukaryotic cells that can easily beRead more
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Catalog NumberQuantity
1644775010 x 15 mL
164471001 mL
1644750015 mL
Catalog number 16447750
Price (EUR)
-
Quantity:
10 x 15 mL
FreeStyle™ MAX Reagent is a proprietary, animal origin–free formulation for transfecting plasmid DNA into eukaryotic cells that can easily be scaled up to produce large amounts of recombinant proteins. FreeStyle™ MAX Reagent allows the highest expression levels and transfection rates with lowest cytotoxicity in bio-production applications, and is specifically formulated for use with:

• FreeStyle™ 293-F Cells (suspension human embryonal kidney cells, Cat. No. R790-07) in serum-free FreeStyle™ 293 Expression Medium (Cat. No. 12338-018)
• FreeStyle™ CHO-S Cells (suspension Chinese Hamster Ovary cells, Cat. No. R800-07) in serum-free FreeStyle™ CHO Expression Medium (Cat. No. 12651-014)
• DG44 Cells (DHFR– suspension CHO cells) in CD DG44 Medium with 8 mM L-glutamine and 18 mL/L 10% Pluronic™ F-68 (Cat. No. A11000-01)

Product use
FreeStyle™ MAX Reagent is intended for use with the FreeStyle™ MAX 293 Expression System (Cat. No. K9000-10) and the FreeStyle™ MAX CHO Expression System (Cat. No. K9000-20).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ClassificationAnimal Origin-Free
For Use With (Application)Protein Expression
High-throughput CompatibilityNot High-throughput Compatible (Manual)
Product LineFreestyle
Product TypeTransfection Reagent
Quantity10 x 15 mL
Serum CompatibleYes
Shipping ConditionWet Ice
Cell TypeEstablished Cell Lines
FormatTube
Sample TypePlasmid DNA
Transfection TechniqueLipid-based Transfection
Unit SizeEach
Contents & Storage
Each kit contains 10 units. Each unit contains one vial of reagent. Store at +4°C. FreeStyle™ MAX is guaranteed stable for 6 months when stored properly.

Frequently asked questions (FAQs)

What is the advantage of FreeStyle Max Reagent over 293fectin Transfection Reagent?

Both FreeStyle Max Reagent and 293fectin Transfection Reagent provide similar levels of high transfection efficiency; however, FreeStyle Max Reagent has lower cytotoxicity and hence results in higher protein yields. Additionally, FreeStyle Max Reagent is animal-origin free.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can FreeStyle 293-F cells grown in FreeStyle 293 expression medium be transfected with ExpiFectamine293 Reagent along with enhancers? Is so, will the transfection efficiency be higher than that with FreeStyle Max Reagent?

You should be able to use the ExpiFectamine293 Reagent for transfection of Freestyle 293-F cells grown in FreeStyle 293 Expression Medium; however, the enhancers will provide only a little boost in expression as they are designed to work with higher density cultures that FreeStyle 293 Expression Medium cannot support.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

What is the recommended ratio of DNA and transfection reagent when transfecting Freestyle cells?

The optimal amount will vary, but we recommend starting with a 1:1 ratio of DNA and lipid. For example, 1.25 ug DNA and 1.25 ul of the reagent for one ml of suspension cells.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

How does FreeStyle MAX reagent compare to Lipofectamine 2000 and 293fectin reagents?

Lipofectamine 2000 is designed for adherent cells. FreeStyle and 293fectin reagents are designed for suspended Hek293 and CHO cells. For transfecting 293 suspension cells, FreeStyle MAX reagent yields transfection efficiency comparable to Lipofectamine 2000 and 293fectin reagents. However, Lipofectamine 2000 and 293fectin reagents do not work well for CHO suspension cells.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

What's the transfection efficiency for FreeStyle MAX reagent?

In general, the transfection efficiency for FreeStyle MAX reagent is 65% when used with suspension CHO and 293 cells.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

View all FreeStyle™ MAX Reagent, 10 x 15 mL FAQs

Citations & References (1)

Citations & References
Abstract
Transient transfection factors for high-level recombinant protein production in suspension cultured mammalian cells.
Authors:Liu C, Dalby B, Chen W, Kilzer JM, Chiou HC,
Journal:Mol Biotechnol
PubMed ID:18327552
'The efficient transfection of cloned genes into mammalian cells system plays a critical role in the production of large quantities of recombinant proteins (r-proteins). In order to establish a simple and scaleable transient protein production system, we have used a cationic lipid-based transfection reagent-FreeStyle MAX to study transient transfection in ... More