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Citations & References (10)
Invitrogen™
BioPrime™ DNA Labeling System
The BioPrime™ DNA Labeling System is specifically designed for use in the preparation of biotinylated probes. Random primers (octamers) areRead more
| Catalog Number | Quantity |
|---|---|
| 18094011 | 30 Reactions |
Catalog number 18094011
Price (EUR)
690,00
Each
Quantity:
30 Reactions
Price (EUR)
690,00
Each
The BioPrime™ DNA Labeling System is specifically designed for use in the preparation of biotinylated probes. Random primers (octamers) are annealed to the denatured DNA template and extended by Klenow fragment in the presence of biotin-14-dCTP to produce sensitive biotinylated-DNA probes for use in the nonradioactive detection of DNA and RNA. With the BioPrime™ DNA Labeling System, considerable net DNA synthesis occurs resulting in a 10–40 fold amplification of the probe. This product is particularly well suited for situations in which the DNA is limited. Probes made with this product have been used for hybridization to Southern and northern blots, plaque and colony lifts, and for in situ hybridization to chromosome spreads. The BioPrime™ DNA Labeling System:
• Requires less template DNA than nick translation
• Amplifies template to provide an increased amount of biotinylated probe
• Labels 50 to 500 ng of template DNA in one reaction
• Requires less template DNA than nick translation
• Amplifies template to provide an increased amount of biotinylated probe
• Labels 50 to 500 ng of template DNA in one reaction
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Includes Label or DyeYes
Labeling MethodDirect Labeling
Product LineBioPrime
Product TypeDNA Labeling System
Quantity30 Reactions
Shipping ConditionApproved for shipment on Wet or Dry Ice
Detection MethodBiotin-based
Final Product TypeProbes (Labeled DNA)
Labeling TargetDNA (General), Genomic DNA
Label or DyeBiotin
Unit SizeEach
Contents & Storage
• 700 μl 2.5X Random Primers Solution
• 175 μl 10X dNTP Mixture
• 100 μg Control DNA
• 35 μl Klenow Fragment (40 U/μl)
• 500 μl Stop Buffer
• 1.25 ml Distilled Water
Store at -20°C in a non-frost-free freezer.
• 175 μl 10X dNTP Mixture
• 100 μg Control DNA
• 35 μl Klenow Fragment (40 U/μl)
• 500 μl Stop Buffer
• 1.25 ml Distilled Water
Store at -20°C in a non-frost-free freezer.
Frequently asked questions (FAQs)
Can exo-Klenow be purchased separately from your array labeling kits?
Citations & References (10)
Citations & References
Abstract
A whole-genome mouse BAC microarray with 1-Mb resolution for analysis of DNA copy number changes by array comparative genomic hybridization.
Journal:Genome Res
PubMed ID:14707179
'Microarray-based comparative genomic hybridization (CGH) has become a powerful method for the genome-wide detection of chromosomal imbalances. Although BAC microarrays have been used for mouse CGH studies, the resolving power of these analyses was limited because high-density whole-genome mouse BAC microarrays were not available. We therefore developed a mouse BAC
DNA replication origins fire stochastically in fission yeast.
Journal:Mol Biol Cell
PubMed ID:16251353
'DNA replication initiates at discrete origins along eukaryotic chromosomes. However, in most organisms, origin firing is not efficient; a specific origin will fire in some but not all cell cycles. This observation raises the question of how individual origins are selected to fire and whether origin firing is globally coordinated
A virulence and antimicrobial resistance DNA microarray detects a high frequency of virulence genes in Escherichia coli isolates from Great Lakes recreational waters.
Journal:Appl Environ Microbiol
PubMed ID:16751532
'Escherichia coli is generally described as a commensal species with occasional pathogenic strains. Due to technological limitations, there is currently little information concerning the prevalence of pathogenic E. coli strains in the environment. For the first time, using a DNA microarray capable of detecting all currently described virulence genes and
Microarray deacetylation maps determine genome-wide functions for yeast histone deacetylases.
Journal:Cell
PubMed ID:12086601
'Yeast contains a family of five related histone deacetylases (HDACs) whose functions are known at few genes. Therefore, we used chromatin immunoprecipitation and intergenic microarrays to generate genome-wide HDAC enzyme activity maps. Rpd3 and Hda1 deacetylate mainly distinct promoters and gene classes where they are recruited largely by novel mechanisms.
Characterization of the genome composition of Bartonella koehlerae by microarray comparative genomic hybridization profiling.
Journal:J Bacteriol
PubMed ID:16109957
Bartonella henselae is present in a wide range of wild and domestic feline hosts and causes cat-scratch disease and bacillary angiomatosis in humans. We have estimated here the gene content of Bartonella koehlerae, a novel species isolated from cats that was recently identified as an agent of human endocarditis. The