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Citations & References (9)
Invitrogen™
ElectroMAX™ DH10B Cells
ElectroMAX DH10B Cells are electrocompetent E. coli cells offering the highest transformation efficiencies of >1 x 1010 cfu/μg plasmid DNA.Read more
| Catalog Number | Quantity |
|---|---|
| 18290015 | 5 x 100 μL |
Catalog number 18290015
Price (EUR)
445,00
Each
Quantity:
5 x 100 μL
Price (EUR)
445,00
Each
ElectroMAX DH10B Cells are electrocompetent E. coli cells offering the highest transformation efficiencies of >1 x 1010 cfu/μg plasmid DNA. The high transformation efficiencies make these cells ideal for cloning both prokaryotic and eukaryotic genomic DNA and efficient plasmid rescue from eukaryotic genomes.
ElectroMAX DH10B Cells are suitable for construction of gene banks, generation of cDNA libraries using plasmid-derrived vectors, and for situations when DNA is limited. They are ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction.
ElectroMAX DH10B Cells provide:
• High-efficiency transformation to maximize clones
• Enhanced genetic markers for cDNA or gDNA cloning capabilities
ElectroMAX DH10B Cell features
• Φ80lacZΔM15 marker that provides α-complementation of the β-galactosidase gene allowing blue/white screening on agar plates containing X-gal or Bluo-gal
• mcrA genotypic marker and mcrBC, mrr deletion that allow cloning DNA that contains methylcytosine and methyladenine
• endA1 mutation that enables increased plasmid yield and quantity
Genotype
F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139Δ(ara, leu)7697 galU galK λ-rpsL nupG
Get more detailed information about specific Genotypes and Genetic Markers of our E. coli Competent Cells.
Find the strain and format that you need
DH10B cells are available in both electrocompetent and chemically competent formats.
ElectroMax DH10B T1R Cells and MegaX DH10B T1R Electrocomp Cells offer the benefit of T1 and T5 phage resistance.
Note: A high-voltage electroporation apparatus capable of generating field strengths of 16 kV/cm is required.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Antibiotic Resistance BacterialYes (Streptomycin)
Blue/White ScreeningYes (lacZΔM15)
Cloning Methylated DNAYes (mcrA)
Cloning Unstable DNANot suitable for cloning unstable DNA
Contains F' EpisomeNo
High-throughput CompatibilityLow
Improves Plasmid QualityYes (endA1)
PlasmidMay be used for plasmids >20 kb
Preparing Unmethylated DNANo
Product LineElectroMAX
Product TypeElectrocompetent Cells
Quantity5 x 100 μL
Reduces RecombinationYes (recA1)
Shipping ConditionDry Ice
T1 Phage - Resistant (tonA)No
Transformation Efficiency LevelHigh Efficiency (>1 x 109 cfu/μg)
FormatTube
SpeciesE. coli (K12)
Unit SizeEach
Contents & Storage
• ElectroMAX DH10B Cells (5 x 100 μL)
Store Competent Cells at –80°C.
• pUC19 DNA (50 μL at 10 pg/μL)
Store pUC19 DNA at –20°C.
• S.O.C. medium (2 x 6 mL)
Store S.O.C. Medium at 4°C or room temperature.
Store Competent Cells at –80°C.
• pUC19 DNA (50 μL at 10 pg/μL)
Store pUC19 DNA at –20°C.
• S.O.C. medium (2 x 6 mL)
Store S.O.C. Medium at 4°C or room temperature.
Citations & References (9)
Citations & References
Abstract
Biosensor detection systems: engineering stable, high-affinity bioreceptors by yeast surface display.
Journal:Methods Mol Biol
PubMed ID:19159105
'Over the past two decades, the field of biosensors has been developing fast, portable, and convenient detection tools for various molecules of interest, both biological and environmental. Although much attention is paid to the transduction portion of the sensor, the actual bioreceptor that binds the ligand is equally critical. Tight,
Mutations in Mlph, encoding a member of the Rab effector family, cause the melanosome transport defects observed in leaden mice.
Journal:Proc Natl Acad Sci U S A
PubMed ID:11504925
'The d, ash, and ln coat color mutations provide a unique model system for the study of vesicle transport in mammals. All three mutant loci encode genes that are required for the polarized transport of melanosomes, the specialized, pigment-containing organelles of melanocytes, to the neighboring keratinocytes and eventually into coat
Association of syncoilin and desmin: linking intermediate filament proteins to the dystrophin-associated protein complex.
Journal:J Biol Chem
PubMed ID:11694502
'We recently identified a novel protein called syncoilin, a putative intermediate filament protein that interacts with alpha-dystrobrevin, a member of the dystrophin-associated protein complex. Syncoilin is found at the neuromuscular junction, sarcolemma, and Z-lines and is thought to be important for muscle fiber integrity. Based on the similar protein structure
Identification of diverse nerve growth factor-regulated genes by serial analysis of gene expression (SAGE) profiling.
Journal:Proc Natl Acad Sci U S A
PubMed ID:10984536
'Neurotrophic factors such as nerve growth factor (NGF) promote a wide variety of responses in neurons, including differentiation, survival, plasticity, and repair. Such actions often require changes in gene expression. To identify the regulated genes and thereby to more fully understand the NGF mechanism, we carried out serial analysis of
Altering the substrate specificity of cephalosporin acylase by directed evolution of the Beta -subunit.
Journal:J Biol Chem
PubMed ID:12198140
'Using directed evolution, we have selected an adipyl acylase enzyme that can be used for a one-step bioconversion of adipyl-7-aminodesacetoxycephalosporanic acid (adipyl-7-ADCA) to 7-ADCA, an important compound for the synthesis of semisynthetic cephalosporins. The starting point for the directed evolution was the glutaryl acylase from Pseudomonas SY-77. The gene fragment