SMPB (succinimidyl 4-(p-maleimidophenyl)butyrate)
SMPB (succinimidyl 4-(p-maleimidophenyl)butyrate)
Citations & References (4)
Thermo Scientific™

SMPB (succinimidyl 4-(p-maleimidophenyl)butyrate)

Thermo Scientific Pierce SMPB is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of aRead more
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Catalog NumberQuantity
2241650 mg
Catalog number 22416
Price (EUR)
263,00
Each
Add to cart
Quantity:
50 mg
Price (EUR)
263,00
Each
Add to cart
Thermo Scientific Pierce SMPB is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length aromatic spacer arm (11.6 angstroms).

Features of SMPB:

• Reactive groups: NHS ester and maleimide
• Reactive towards: amino and sulfhydryl groups
• Extended chain analog of MBS
• Non-cleavable
• Water-insoluble (dissolve first in DMF or DMSO)
• Conjugates formed with SMPB are more stable in serum than SPDP conjugates

Product References:
Crosslinker Application Guide -- search for recent literature references for this product

Related Products
Sulfo-SMPB (sulfosuccinimidyl 4-(N-maleimidophenyl)butyrate)
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell PermeabilityYes
DescriptionSMPB
FormPowder
Labeling MethodChemical Labeling
Molecular Weight (g/mol)356.33
PEGylatedNo
Product LinePierce
Quantity50 mg
Reactive MoietyMaleimide, NHS Ester
Shipping ConditionAmbient
SolubilityDMF, DMSO
Spacer Arm Length11.6 Å
Water SolubleNo
Chemical ReactivityAmine-Sulfhydryl
CleavableNo
Crosslinker TypeHeterobifunctional
FormatStandard
Product TypeCrosslinker
SpacerMedium (10 to 30 Å)
Unit SizeEach
Contents & Storage
Upon receipt store desiccated at 4°C.

Frequently asked questions (FAQs)

Can you provide the shelf-life for SMPB (succinimidyl 4-(p-maleimidophenyl)butyrate)?

SMPB (succinimidyl 4-(p-maleimidophenyl)butyrate) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Citations & References (4)

Citations & References
Abstract
Covalent attachment of synthetic DNA to self-assembled monolayer films.
Authors:Chrisey LA, Lee GU, O'Ferrall CE
Journal:Nucleic Acids Res
PubMed ID:8760890
'The covalent attachment of thiol-modified DNA oligomers; to self-assembled monolayer silane films on fused silica and oxidized silicon substrates is described. A heterobifunctional crosslinking molecule bearing both thiol- and amino-reactive moieties was used to tether a DNA oligomer (modified at its terminus with a thiol group) to an aminosilane film ... More
Decorin is a novel antagonistic ligand of the Met receptor.
Authors:Goldoni S, Humphries A, Nyström A, Sattar S, Owens RT, McQuillan DJ, Ireton K, Iozzo RV
Journal:J Cell Biol
PubMed ID:19433454
'Decorin, a member of the small leucine-rich proteoglycan gene family, impedes tumor cell growth by down-regulating the epidermal growth factor receptor. Decorin has a complex binding repertoire, thus, we predicted that decorin would modulate the bioactivity of other tyrosine kinase receptors. We discovered that decorin binds directly and with high ... More
Dendritic cell-directed CTLA-4 engagement during pancreatic beta cell antigen presentation delays type 1 diabetes.
Authors:Karumuthil-Melethil S, Perez N, Li R, Prabhakar BS, Holterman MJ, Vasu C
Journal:J Immunol
PubMed ID:20483724
The levels of expression of alternatively spliced variants of CTLA-4 and insufficient CTLA-4 signaling have been implicated in type 1 diabetes. Hence, we hypothesized that increasing CTLA-4-specific ligand strength on autoantigen-presenting dendritic cells (DCs) can enhance ligation of CTLA-4 on T cells and lead to modulation of autoreactive T cell ... More
Visualizing dynamic cytoplasmic forces with a compliance-matched FRET sensor.
Authors:Meng F, Sachs F
Journal:J Cell Sci
PubMed ID:21172803
Mechanical forces are ubiquitous modulators of cell activity but little is known about the mechanical stresses in the cell. Genetically encoded FRET-based force sensors now allow the measurement of local stress in specific host proteins in vivo in real time. For a minimally invasive probe, we designed one with a ... More