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Gibco™
DMEM/F-12, powder
Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth ofRead more
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| Catalog Number | Quantity |
|---|---|
| 32500043 also known as 32500-043 | 50 L |
| 32500035 | 10 L |
Catalog number 32500043
also known as 32500-043
Price (EUR)
132,00
Each
Quantity:
50 L
Price (EUR)
132,00
Each
Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM / F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of Gibco™ DMEM / F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM / F-12 is modified as follows:
| With | Without |
| • L-glutamine | • HEPES |
| • Phenol Red | • Sodium Bicarbonate |
The complete formulation is available.Gibco™ DMEM / F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components.
DMEM / F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM / F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM / F-12 uses a sodium bicarbonate buffer system and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
Specifications
Culture EnvironmentCO2
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Osmolality250 - 290 mOsm/kg
Product LineGibco
Product TypeDMEM/F-12
Quantity50 L
Shelf Life36 Months
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
Culture TypeMammalian Cell Culture
FormPowder
Serum LevelStandard Serum Supplementation
With AdditivesGlutamine, Phenol Red, Sodium Pyruvate
Without AdditivesNo HEPES, No Sodium Bicarbonate
pH6.2 to 6.6
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C.
Shipping conditions: Ambient.
Shelf life: 36 months from date of manufacture.
Shipping conditions: Ambient.
Shelf life: 36 months from date of manufacture.