DAB EASY tablets, for immunohistochemistry, 10 mg/tablet

DAB, or 3,3-diaminobenzidine tetrahydrochloride, EASY tablets for immunohistochemistry, CAS # 7411-49-6, is a chromogenic substrate used to observe peroxidase activity. In immunohistochemistry, DAB is used to catalyze peroxidase-bound antibodies in order to visualize protein presence. | CAS: 7411-49-6

Catalog Number	Quantity
328005000	500 mg

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Catalog number 328005000

Price (EUR)

309,00

Quantity:

500 mg

Request bulk or custom format

Price (EUR)

309,00

Chemical Identifiers

CAS7411-49-6

IUPAC Nametetrahydrogen [1,1'-biphenyl]-3,3',4,4'-tetramine tetrachloride

Molecular FormulaC12H18Cl4N4

InChI KeyKJDSORYAHBAGPP-UHFFFAOYSA-N

SMILES[H+].[H+].[H+].[H+].[Cl-].[Cl-].[Cl-].[Cl-].NC1=CC=C(C=C1N)C1=CC=C(N)C(N)=C1

Specifications

Assay9 to 11 mg/tablet

pH6.0 to 7.5

Appearance (Color)Light grayish white

Appearance (Form)Tablet

This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

3,3′-Diaminobenzidine is a derivative of benzene and the precursor to polybenzimidazole

Applications

DAB is mainly used to visualize peroxidase activity. During this chemical reaction, DAB is a hydrogen donor in the presence of peroxide. When DAB is oxidized, an insoluble brown end-product is produced.
DAB has been used for staining and oxidative detection of mitochondria
It has also been utilized for electron microscopy applications

RUO – Research Use Only

Literature References: Beard, M.E.; Novikoff, A.B. Distribution of Peroxisomes (Microbodies) in the Nephron of the Rat: A Cytochemical Study. J Cell Biol. 1969; 42(2): 501-518.Cammer, W.; Moore, C.L. Oxidation of 3,3'-diaminobenzidine by rat liver mitochondria. Biochem. 1973; 12(13): 2502-2509.

Frequently asked questions (FAQs)

How should I reconstitute DAB EASY tablets, for immunohistochemistry, 10 mg/tablet (Cat. No. 328005000)?

1. Remove the bottle from the refrigerator and allow to stand at room temperature (~20 degrees C) for 15-30 min.
2. Dissolve five tablets containing 10 mg DAB in 10 mL of distilled water at room temperature. Do not use metal forceps during handling of the tablets, since metal ions catalyze the oxidation process. Protect the solution from direct sunlight.
3. Some fine particulate matter will remain in the suspension even after the solution is thoroughly mixed. This will not affect your results, however if preferred, the solution can be filtered using Whatman No. 2 paper.
4. Add 4 urea peroxide tablets (Acros Cat. No. 32807) to 10 mL of the substrate solution. Alternatively add 40 µL 30% hydrogen peroxide (H2O2) and mix. This solution should be used within 2 hours.