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Thermo Scientific™
SuperSignal™ West Pico PLUS Chemiluminescent Substrate
Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.
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| Catalog Number | Quantity |
|---|---|
| 34580 | 500 mL |
| 34579 | 20 mL |
| 34577 | 200 mL |
| 34580X4 | 4 x 500 mL |
| 34578 | 1 L |
| 34578X4 | 4 x 1 L |
Catalog number 34580
Price (EUR)
256,65
線上優惠
295,00Save 38,35 (13%)
Each
Quantity:
500 mL
Price (EUR)
256,65
線上優惠
295,00Save 38,35 (13%)
Each
Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.
SuperSignal West Pico PLUS Chemiluminescent Substrate characteristics include:
- Sensitivity: picogram to femtogram sensitivity
- Stability: 8-hr working solution stability; 1-year kit stability at room temperature
- Compatibility: nitrocellulose and PVDF membranes
- Signal duration: 6 to 24 hours
- Recommended primary antibody concentration: 1:1000–1:5000 dilution (0.2–1.0 μg/mL)
- Recommended secondary antibody concentration: 1:20,000–100,000 dilution (10–50 ng/mL)
SuperSignal West Pico PLUS substrate is designed to provide excellent signal intensity and sensitivity for western blotting with HRP conjugates. The intensity of the light emission combined with the exceptional duration allows for the acquisition of multiple exposures to more easily obtain publication-quality blot images. SuperSignal West Pico PLUS substrate is compatible with different membranes, blocking reagents, and a wide range of antibody dilutions, making it an ideal choice for most western blotting applications.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ConjugateHRP
Kit ContentsLuminol/Enhancer, 250 mL, Stable Peroxide Buffer, 250 mL
Recommended Antibody Concentrations2°: 1:20-100 K (10-50 ng/mL), 1°: 1:1 K (0.2-1 μg/mL)
SensitivityPicogram to high femtogram-level
Shipping ConditionRoom Temperature
Signal Duration6 to 24 hr.
Sufficient For60 Mini-Gel Blots, 5000 cm2 of Membrane
Detection MethodChemiluminescence
For Use With (Application)Western Blot
FormLiquid
Membrane CompatibilityNitrocellulose, PVDF
Product LineSuperSignal
Product TypeSubstrate
Quantity500 mL
SubstrateHorseradish Peroxidase
Unit SizeEach
Contents & Storage
Sufficient for 60 mini-gel blots; 5000 cm2 of membrane
• SuperSignal West Pico PLUS Luminol/Enhancer, 250 mL
• SuperSignal West Pico PLUS Stable Peroxide, 250 mL
Store at room temperature.
• SuperSignal West Pico PLUS Luminol/Enhancer, 250 mL
• SuperSignal West Pico PLUS Stable Peroxide, 250 mL
Store at room temperature.