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Citations & References (159)
Applied Biosystems™
MicroSEQ™ 500 16S rDNA PCR Kit
The MicroSEQ™ 500 16S rDNA PCR Kit is the PCR component of the MicroSEQ™ 500 16S rDNA Bacterial Identification System,Read more
| Catalog Number | Quantity |
|---|---|
| 4348228 | 1 kit |
Catalog number 4348228
Price (EUR)
1.526,00
Each
Quantity:
1 kit
Price (EUR)
1.526,00
Each
The MicroSEQ™ 500 16S rDNA PCR Kit is the PCR component of the MicroSEQ™ 500 16S rDNA Bacterial Identification System, which provides an easy-to-use DNA sequence-based method to identify most bacteria. PCR-amplified products generated with this kit can be sequenced using the MicroSEQ™ 500 16S rDNA Sequencing Kit. The two kits together permit the quick and convenient amplification and sequencing of 500 base pairs of the 16S rRNA gene, which can then be compared to the MicroSEQ™ 16S rDNA 500 Library to obtain an identity match. This kit does not include a copy of the protocol, nor a Quick Reference Card.
• Sequence-based measurement ensures you get reproducible results in under 24 hours.
• Growth requirement-independent measurement enables you to identify bacteria using objective criteria.
• Universal domain-level primers eliminate up-front optimization.
• MicroSEQ ID Analysis Software ensures accurate species identification.
• Customizable libraries give you the flexibility to create new libraries and search validated libraries that contain the most frequently encountered organisms.
• Requires minimal training-same work flow as the MicroSEQ D2 LSU fungal kit.
The MicroSEQ™ ID System: A Complete, Integrated Solution
Streamlining every step in microbial identification, Applied Biosystems MicroSeq System combines the advantages of MicroSEQ ID Software with easy PrepMan Ultra sample preparation reagents and protocols, MicroSeq bacterial and fungal application kits, and industry-leading thermal cyclers and sequencing systems. To ensure optimal performance and system-wide integration, we develop and test all components of the system together.
For Research Use Only. Not for use in diagnostics procedures.
• Sequence-based measurement ensures you get reproducible results in under 24 hours.
• Growth requirement-independent measurement enables you to identify bacteria using objective criteria.
• Universal domain-level primers eliminate up-front optimization.
• MicroSEQ ID Analysis Software ensures accurate species identification.
• Customizable libraries give you the flexibility to create new libraries and search validated libraries that contain the most frequently encountered organisms.
• Requires minimal training-same work flow as the MicroSEQ D2 LSU fungal kit.
The MicroSEQ™ ID System: A Complete, Integrated Solution
Streamlining every step in microbial identification, Applied Biosystems MicroSeq System combines the advantages of MicroSEQ ID Software with easy PrepMan Ultra sample preparation reagents and protocols, MicroSeq bacterial and fungal application kits, and industry-leading thermal cyclers and sequencing systems. To ensure optimal performance and system-wide integration, we develop and test all components of the system together.
For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Equipment)GeneAmp 9700, Veriti Thermal Cycler
LibrariesCustomizable, Bacterial Library (> 2000 species)
Product LineMicroSEQ
Product TypePCR Kit
Quantity1 kit
Sample TypeDNA (Genomic)
Shipping ConditionDry Ice
SoftwareMicroSEQ™ ID Analysis Software
Target1st 500 bp of 16S rRNA gene
Target Organism ClassBacteria
TechniquePCR Amplification, SBT (Sequence-Based Typing)
Detection MethodPrimer-probe
For Use With (Application)Microbial Detection
Unit SizeEach
Contents & Storage
Each kit contains enough PCR Master Mix to perform 50 PCR amplifications, 5 negative control assays, and 5 positive control assays. One tube of positive control E. coli DNA, and one of water (for use as a negative control) are also included. Store at -15 to -25°C upon reciept. After first use, store at 2–8 °C in a PCR Clean Room.
Frequently asked questions (FAQs)
What is the sensitivity of the MicroSeq 500 kits?
Citations & References (159)
Citations & References
Abstract
Structures of aldouronic acids liberated from kenaf xylan by endoxylanases from Streptomyces sp.
Journal:
PubMed ID:
'Streptomyces sp. SK519 was cultivated in the medium containing 0.5% birch wood xylan to produce extracellular xylanase. The xylanase was isolated from the culture supernatant by ammonium sulfate precipitation and partially purified by absorbing on the insoluble xylan. Alkali-extracted kenaf glucuronoylan of which main chain primarily consisting of (1-4)-linked β-D-xylopyranose
Pseudomonas panipatensis sp. nov., isolated from an oil-contaminated site
Journal:
PubMed ID:
'A Gram-negative, motile, rod-shaped, non-sporulating, aerobic bacterial strain (Esp-1(T)) was isolated from oil-contaminated soil of Panipat Oil Refinery, India, and its taxonomic position was determined using a polyphasic approach. Strain Esp-1(T) grew in the presence of 2% NaCl at 30 °C and was characterized chemotaxonomically by having C(16:0) as the
Multilocus DNA sequence comparisons rapidly identify pathogenic molds.
Journal:
PubMed ID:
'The increasing incidence of opportunistic fungal infections necessitates rapid and accurate identification of the associated fungi to facilitate optimal patient treatment. Traditional phenotype-based identification methods utilized in clinical laboratories rely on the production and recognition of reproductive structures, making identification difficult or impossible when these structures are not observed. We
Species distribution and antimicrobial resistance of enterococci isolated from surface and ocean water
Journal:
PubMed ID:
'Aims: The species identification and antimicrobial resistance profiles were determined for enterococci isolated from Southern California surface and ocean waters. Methods and Results: Species identification was determined for 1413 presumptive Enterococcus isolates from urban runoff, bay, ocean and sewage water samples. The most frequently isolated species were Enterococcus faecalis, Enterococcus
Multicenter evaluation of the new VITEK 2 advanced colorimetric yeast identification card.
Journal:
PubMed ID:
'The performance of the new VITEK 2 Advanced Colorimetry yeast identification (YST) card for use with the VITEK 2 system (bioMerieux, Inc., Hazelwood, MO) was compared to that of the API 20C AUX (API) system (bioMerieux SA, Marcy-l''Etoile, France) in a multicenter evaluation. A total of 12 quality control, 64