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Citations & References (2)
Gibco™
Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (100X)
Gibco™ Insulin,-Transferrin-Selenium-Ethanolamine (ITS-X) is used as a basal medium supplement in order to reduce the amount of fetal bovine serumRead more
| Catalog Number | Quantity |
|---|---|
| 51500056 | 10 mL |
Catalog number 51500056
Price (EUR)
85,00
Each
Quantity:
10 mL
Price (EUR)
85,00
Each
Gibco™ Insulin,-Transferrin-Selenium-Ethanolamine (ITS-X) is used as a basal medium supplement in order to reduce the amount of fetal bovine serum (FBS) used to culture cells. ITS-X solution is routinely used, in combination with 2 to 4% FBS, to support cell types that require ethanolamine, such as bronchial epithelial cells.
Insulin promotes glucose and amino acid uptake, lipogenesis, intracellular transport, and the synthesis of proteins and nucleic acids. Transferrin is an iron carrier and may also help to reduce toxic levels of oxygen radicals and peroxide. Selenium, as sodium selenite is a co-factor for glutathione peroxidase and other proteins that is used as an anti-oxidant in media. Ethanolamine is a precursor of phospho-glycerides which are essential to the structure of the plasma membrane and cellular organelles.
The complete formulation is available.
cGMP Manufacturing and Quality System
Gibco™ ITS-X is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeMammalian Cells
Concentration100 X
Quantity10 mL
Shelf Life18 Months
Shipping ConditionRoom Temperature
FormLiquid
Product TypeTransferrin
SterilitySterile-filtered
With AdditivesInsulin
Unit SizeEach
Contents & Storage
Storage conditions: 2°C to 8°C
Shipping conditions: Room temperature
Shelf life: 18 months from date of manufacture
Shipping conditions: Room temperature
Shelf life: 18 months from date of manufacture
Frequently asked questions (FAQs)
What is the buffer composition of the various Insulin-Transferrin-Selenium (ITS) (100X) and Insulin-Transferrin-Selenium (ITS) Select (100X) supplements?
What is the difference between Insulin-Transferrin-Selenium (ITS) (100X) and Insulin-Transferrin-Selenium (ITS) Select (100X) supplements?
What are the individual concentrations of insulin, transferrin, and selenium in the Insulin-Transferrin-Selenium (ITS) (100X) supplements?
Can I store aliquots of Insulin-Transferrin-Selenium (ITS) (100X) supplements at -20 degrees C without compromising the integrity of the product?
What is the source of transferrin in Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (100X) (Cat. No. 51500056)?
Citations & References (2)
Citations & References
Abstract
Directed differentiation of pancreatic δ cells from human pluripotent stem cells.
Journal:Nature communications
PubMed ID:39068220
Dysfunction of pancreatic δ cells contributes to the etiology of diabetes. Despite their important role, human δ cells are scarce, limiting physiological studies and drug discovery targeting δ cells. To date, no directed δ-cell differentiation method has been established. Here, we demonstrate that fibroblast growth factor (FGF) 7 promotes pancreatic
Protection of Primary Dopaminergic Midbrain Neurons by GPR139 Agonists Supports Different Mechanisms of MPP(+) and Rotenone Toxicity.
Journal:Front Cell Neurosci
PubMed ID:27445691
'The G-protein coupled receptor 139 (GPR139) is expressed specifically in the brain in areas of relevance for motor control. GPR139 function and signal transduction pathways are elusive, and results in the literature are even contradictory. Here, we examined the potential neuroprotective effect of GPR139 agonism in primary culture models of