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Pierce™ C18 Spin Tips & Columns
Thermo Scientific™

Pierce™ C18 Spin Tips & Columns

Thermo Scientific Pierce C18 Spin Columns (10–150 μL) and Spin Tips (10–100 μL) effectively remove interfering contaminants, while also maintaining excellent binding and recovery of peptide samples for use upstream of mass spectrometry and other high sensitivity detection methods.
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Catalog NumberQuantityProduct Type
8485096 TipsSpin Tip
8987025 ColumnsColumn
8987350 ColumnsColumn
8778296 x 10 μL TipsPipette Tip
8778496 x 100 μL TipsPipette Tip
C18485002096 x 20 μL TipsSpin Tip
C18485020096 x 200 μL TipsSpin Tip
Catalog number 84850
Price (EUR)
332,65
Online Exclusive
382,00
Save 49,35 (13%)
Each
Quantity:
96 Tips
Product Type:
Spin Tip
Request bulk or custom format
Price (EUR)
332,65
Online Exclusive
382,00
Save 49,35 (13%)
Each

C18 Spin Tips for Peptide Desalting and Cleanup

Pierce™ C18 spin tips are designed for rapid, reproducible peptide desalting, cleanup, and concentration prior to mass spectrometry analysis. Utilizing reversed-phase C18 chemistry, these tips help enable efficient peptide binding and recovery while removing salts, detergents, and other contaminants that can interfere with downstream applications.

Used in combination with Pierce Spin Tip Adaptors (Catalog No. A40011776) and low protein binding microcentrifuge tubes (Catalog No. 90410), C18 spin tips support a simple centrifugation-based workflow that delivers consistent and reliable results across a wide range of sample inputs.

Features of C18 Spin Tips

  • Reliable – proven reversed-phase C18 chemistry
  • Efficient – effectively removes interfering contaminants to improve spectral quality
  • Streamlined – simple workflow using tip adaptor and centrifugation steps

Applications

  • Peptide desalting prior to LC-MS analysis
  • Cleanup following protein digestion workflows
  • Removal of salts, buffers, and contaminants
  • Peptide concentration and sample preparation

How to Use

C18 spin tips use a bind–wash–elute workflow performed by centrifugation. Tips are placed into a tip adaptor seated in a microcentrifuge tube for each step. Simply activate and equilibrate prior to adding your acidified sample. To enhance peptide binding repeat the sample loading step one time. Lastly, wash 2-3 times and elute into a fresh tube using an acetonitrile solution. Refer to Table 1 in the Figures for recommended buffers, volumes and spin speeds for each tip size.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Final Product TypePeptides
For Use With (Equipment)Microcentrifuge, Mass Spectrometer
Quantity96 Tips
Workflow StepSample Clean Up
Detection MethodMass Spectrometry
FormSolid
Product LinePierce
Product TypeSpin Tip
Starting MaterialPeptides, Protease-digested Protein
Unit SizeEach
Contents & Storage
Store at room temperature.

Frequently asked questions (FAQs)

Do you offer the adaptors provided with Pierce C18 Spin Tips (Cat. No. 84850) as a stand-alone product?

Unfortunately, the adaptors provided with Pierce C18 Spin Tips are not sold separately and they are supplied in a smaller quantity because they are meant to be reused.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Do I need to do C18 clean-up of my mass spectrometry sample before phosphopeptide enrichment?

Yes. C18 clean-up is necessary to remove small molecules that may interfere with phosphopeptide enrichment. Drying after clean-up is also necessary to concentrate the dilute samples and remove C18 organic eluting solvent so samples can be dissolved in the optimized buffers for phosphopeptide binding. Alternative binding buffers are not recommended as phosphopeptide yield and specificity will be affected. For maximal performance, ensure that the clean, dried peptide samples are completely solubilized in binding buffer with agitation for at least 10 min.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

How do I determine if my sample is compatible and ready for LC-MS?

Samples prepared using LC/MS grade reagents are suitable for LC-MS; however, particulates and other small molecules can all interfere with liquid chromatography separation and mass spectrometer source ionization. We recommend visual inspection of samples for particulate matter and using Pierce Peptide Desalting Spin Columns (Cat. Nos. 89851, 89852), Pierce C18 Spin Tips (Cat. No. 84850) or an in-line C18 trap column (https://www.thermofisher.com/order/catalog/product/164564-CMD) to remove non-volatile salts before MS analysis.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

I cleaned up my peptide sample using C18 or desalting columns, but I lost most of my peptides. What went wrong?

Peptides do not bind well to reversed phase resins at neutral pH or in the presence of organic solvents (e.g., acetonitrile). Acidify protein digest samples using formic acid or trifluoroacetic acid (TFA) to pH <3 before desalting. Ensure that samples do not contain organic solvents before and after clean-up by drying them down using a SpeedVac concentrator or equivalent.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

If I already removed small contaminants and detergents at the protein level, do I still need to desalt my peptides prior to mass spectrometry analysis?

Yes. We recommend performing additional cleanup after protein digestion to remove any residual salts or partially digested proteins using Pierce Peptide Desalting Spin Columns (Cat. Nos. 89851, 89852), Pierce C18 Spin Tips (Cat. No. 84850) or an in-line C18 trap column (https://www.thermofisher.com/order/catalog/product/164564-CMD).

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.