StemPro™ hESC SFM
StemPro™ hESC SFM
Gibco™

StemPro™ hESC SFM

StemPro hESC SFM is a serum- and feeder-free medium specially formulated to support the growth and expansion of pluripotent stem cells (hESCs and iPSCs). It has been shown to support PSC growth for >50 passages without any signs of karyotypic abnormalities while maintaining differentiation potential.
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Catalog NumberQuantity
A10007011 Kit
Catalog number A1000701
Price (EUR)
532,00
Each
Add to cart
Quantity:
1 Kit
Price (EUR)
532,00
Each
Add to cart

StemPro hESC SFM is a serum- and feeder-free medium specially formulated to support the growth and expansion of pluripotent stem cells (hESCs and iPSCs). It has been shown to support PSC growth for >50 passages without any signs of karyotypic abnormalities while maintaining differentiation potential.

StemPro hESC SFM has been extensively tested and is proven to maintain pluripotency in a growing list of hESC lines, including BG01, BG02, BG03, H1, H9, and BG01V. In addition, it has been used to scale up production of hESCs to over 1 x 1010 cells. StemPro hESC SFM has been shown to support hESC and iPSC growth for >50 passages without any signs of karyotypic abnormalities and to maintain the ability of hESCs to differentiate into all three germ line lineages.

StemPro hESC SFM components

  • StemPro hESC Supplement
  • DMEM/F-12 with GlutaMAX medium
  • Bovine serum albumin 25% (BSA)

Additional components required for complete medium (need to be purchased separately)

  • FGF BASIC (full length) REC HU (Cat. no. PHG0261)
  • 2-Mercaptoethanol (Cat. no. 21985-023)

Applications

Formulated for the growth and expansion of human embryonic and human induced pluripotent stem cells.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeEmbryonic Stem Cells (ESCs)
Manufacturing QualitycGMP for medical devices, 21 CFR Part 820 and ISO 13485
Product LineStemPro
Product TypeStem Cell Media
Quantity1 Kit
Shipping ConditionSupplement - dry ice, Basal - ambient, BSA - ambient
SpeciesHuman
ClassificationSerum-free
Culture TypeFeeder-free Stem Cell Culture (Human, iPS - Induced Pluripotent Stem, Embryonic)
FormLiquid
Serum LevelSerum-free
With AdditivesGlutaMAX, Phenol Red
Unit SizeEach
Contents & Storage
• StemPro™ hESC Supplement, store at -5 to -20°C and protect from light
• DMEM/F-12 with GlutaMAX medium, store at 2 to 8°C and protect from light
• Bovine serum albumin (BSA) 25%, store at 2 to 8°C and protect from light

Frequently asked questions (FAQs)

How do I prepare the StemPRO hESC SFM complete medium?

We recommend to thaw the supplement in the 37°C water bath, aliquot and freeze at -5°C to -20°C. Thaw each aliquot only one additional time. To make 100 ml complete medium, mix 90.8 ml DMEM/F-12 with GlutaMax matrix, 2 ml StemPRO hESC supplement, 7.2 ml 25% BSA solution, 80 ul bFGF (at 10 ug/ml), and 182 ul 2-mercaptoethanol (at 55mM). Store at 2°C-8°C until ready to use, but use the same day.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can StemPRO hESC SFM be used to culture mouse ESCs?

No, we don't recommend StemPro hESC SFM for mouse cells because they showed poor growth and some differentiation. We recommend KnockOut DMEM and KnockOut SR for mouse ESC cultures (catalog numbers 10829-018 and 10828-028 respectively).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can StemPRO hESC SFM be used for new cell line derivations?

Yes, derivations using StemPro hESC SFM have been carried out using either a basement membrane extract (like Geltrex growth factor) or feeder cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What cell lines has StemPRO hESC SFM been tested with?

StemPro hESC SFM has been tested with the following hESC: H1, H9, BG01, BG02, BG03, HUES6, HUES9, Cyth203, CyT49, BG01v, HES-2, HES-3, KhES-1, and TE06. The medium has also been used with the Rhesus monkey line R336.4. Some cells may require a short adaptation time but most do not.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Does StemPRO hESC SFM require the use of a basement membrane matrix?

Yes, we recommend using Geltrex hESC-qualified Reduced Growth Factor basement membrane (catalog number A10480-02), CELLstart (catalog number A10142-01) or similar matrix.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.