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Citations & References (3)
Invitrogen™
AbC™ Anti-Rat/Hamster Bead Kit
The AbC™ Anti-Rat/Hamster Antibody Compensation Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometryRead more
| Catalog Number | Quantity |
|---|---|
| A10389 | 1 kit |
Catalog number A10389
Price (EUR)
373,00
Each
Quantity:
1 kit
Price (EUR)
373,00
Each
The AbC™ Anti-Rat/Hamster Antibody Compensation Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when using fluorochrome-conjugated to rat or hamster antibodies with any laser in flow cytometry.
• Species reactivity—for use with all isotypes of rat and hamster antibody conjugates
• Maximum laser compatibility—for use with all lasers
Accurate compensation is crucial for effective multicolor analysis in flow cytometry. Often, however, cellular-based compensation controls are not completely effective, as many antigens are not highly expressed, and dimly stained cells can lead to inaccurate compensation settings. The AbC™ Anti-Rat/Hamster Bead Kit was developed to address this issue.
View a selection guide for all flow cytometry compensation beads.
How it works
Each kit provides polystyrene microspheres that have fluorescent antibody conjugate capture capacity (positive compensation beads) or are inert (negative compensation beads). After mixing with a fluorophore-conjugated rat or hamster antibody, the two components provide a distinct high-signal positive control with an appropriate negative population that can then be used to set compensation properly regardless of the intensity of the cells in the actual experiment.
Species reactivity
The AbC™ Anti-Rat/Hamster Bead Kit is ideal for compensation with all isotypes of rat (IgG1, IgG2a, IgG2b, IgG2c, IgM) and hamster (Armenian IgG and Syrian IgG) immunoglobulins.
Maximum laser compatibility
All AbC™ Compensation Bead Kits are ideal for use with any excitation source, including the 405 nm laser which has historically provided higher background signals due to auto-fluorescence with other kits on the market.
• Species reactivity—for use with all isotypes of rat and hamster antibody conjugates
• Maximum laser compatibility—for use with all lasers
Accurate compensation is crucial for effective multicolor analysis in flow cytometry. Often, however, cellular-based compensation controls are not completely effective, as many antigens are not highly expressed, and dimly stained cells can lead to inaccurate compensation settings. The AbC™ Anti-Rat/Hamster Bead Kit was developed to address this issue.
View a selection guide for all flow cytometry compensation beads.
How it works
Each kit provides polystyrene microspheres that have fluorescent antibody conjugate capture capacity (positive compensation beads) or are inert (negative compensation beads). After mixing with a fluorophore-conjugated rat or hamster antibody, the two components provide a distinct high-signal positive control with an appropriate negative population that can then be used to set compensation properly regardless of the intensity of the cells in the actual experiment.
Species reactivity
The AbC™ Anti-Rat/Hamster Bead Kit is ideal for compensation with all isotypes of rat (IgG1, IgG2a, IgG2b, IgG2c, IgM) and hamster (Armenian IgG and Syrian IgG) immunoglobulins.
Maximum laser compatibility
All AbC™ Compensation Bead Kits are ideal for use with any excitation source, including the 405 nm laser which has historically provided higher background signals due to auto-fluorescence with other kits on the market.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Diameter (Metric)6 μm
Emissionany lasers (UV to 633/635)
For Use With (Equipment)Flow Cytometer
FormatTube
Quantity1 kit
Shipping ConditionRoom Temperature
Stain ConfigurationSurface
Product LineCompenFlow, Molecular Probes
Product TypeCompensation Beads
Unit SizeEach
Contents & Storage
Contains 1 vial AbC™ anti-rat/hamster capture beads and 1 vial of negative beads.Store at 2°C to 8°C
Frequently asked questions (FAQs)
Does the AbC Anti-Rat/Hamster Bead Kit bind IgG2a kappa or IgG1 lambda rat with the same affinity?
I am performing flow cytometry. My compensation matrix has values over 100. That's not possible is it?
I’ve been told that I need to compensate my data for flow cytometry analysis. What does that mean?
What kind of controls do I need for flow cytometry?
Why should I worry about compensation in flow cytometry analysis?
Citations & References (3)
Citations & References
Abstract
Identification and characterization of intestinal antigen-presenting cells involved in uptake and processing of a nontoxic recombinant chimeric mucosal immunogen based on cholera toxin using imaging flow cytometry.
Journal:
PubMed ID:24197893
'Intragastric immunization with recombinant chimeric immunogen, SBR-CTA2/B, constructed from the saliva-binding region (SBR) of Streptococcus mutans antigen AgI/II and the A2/B subunits of cholera toxin (CT) induces salivary and circulating antibodies against S. mutans that protect against dental caries. We previously found that SBR-CTA2/B activated dendritic cells (DC) in the
Multiparametric analysis of host response to murine cytomegalovirus in MHC class I-disparate mice reveals primacy of Dk-licensed Ly49G2+ NK cells in viral control.
Journal:
PubMed ID:24068668
MHC class I D(k) and Ly49G2 (G2) inhibitory receptor-expressing NK cells are essential to murine CMV (MCMV) resistance in MA/My mice. Without D(k), G2(+) NK cells in C57L mice fail to protect against MCMV infection. As a cognate ligand of G2, D(k) licenses G2(+) NK cells for effector activity. These
Self MHC class I-licensed NK cells enhance adaptive CD8 T-cell viral immunity.
Journal:Blood
PubMed ID:21436069
MHC class I (MHC I) is essential to NK- and T-cell effector and surveillance functions. However, it is unknown whether MHC I polymorphism influences adaptive immunity through NK cells. Previously, we found that MHC I D(k), a cognate ligand for the Ly49G2 inhibitory receptor, was essential to NK control of