Hibernate™-A Medium
Hibernate™-A Medium
Citations & References (2)
Gibco™

Hibernate™-A Medium

Hibernate-A Medium is a specialized CO2-independent medium for neural cells that provides flexibility and control for benchtop experiments and transportation.
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Catalog NumberQuantity
A1247501500 mL
Catalog number A1247501
Price (EUR)
162,00
Each
Add to cart
Quantity:
500 mL
Price (EUR)
162,00
Each
Add to cart

Hibernate-A Medium is a specialized CO2-independent nutrient medium designed for the maintenance and preservation of neural cells, tissues, and slices. Optimized for adult and postnatal neural samples, this medium supports neurons in ambient carbon dioxide conditions for up to 48 hours. This feature provides researchers with enhanced flexibility and control during experiments and transportation. Hibernate-A Medium allows you to run bench-top live cell experiments, such as microscopy, flow cytometry, and other physiology studies.

Benefits

  • CO2 independence—eliminates the need for CO2 incubation for up to 48 hours, simplifying the maintenance and processing of neural cultures and tissues
  • Convenience and flexibility—ideal for experiments that require extended handling times outside of CO2 incubators, offering greater convenience for researchers
  • Transportation of neural samples—provides a reliable medium for the shipment of neural tissues, helping ensure their stability and viability upon arrival
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeNeural Cells
Product LineHibernate
Product TypeCell Culture Medium
Quantity500 mL
Shelf Life24 Months
Shipping ConditionAmbient
Tested ForCytotoxicity, Endotoxin, Sterility, pH, Osmolality
ClassificationSerum-free
Culture TypeMammalian Cell Culture
FormLiquid
Serum LevelSerum-free
SterilitySterile
With AdditivesPhenol Red
Unit SizeEach
Contents & Storage
Store in refrigerator (2°C to 8°C) and protect from light.

Frequently asked questions (FAQs)

What medium can be used to maintain/manipulate neurons at ambient CO2?

We offer Gibco Hibernate Medium for this purpose. When supplemented with Gibco B-27 Supplement and Gibco GlutaMAX-1 Supplement, this medium can allow for the manipulation of neurons at ambient CO2 for at least 48 hours while retaining their viability, and preserve viable brain tissue for up to a month when stored at 4 degrees C. Two Hibernate media are offered:

- Gibco Hibernate-A Medium (Cat. No. A1247501) is formulated for postnatal neurons.
- Gibco Hibernate-E Medium (Cat. No. A1247601) is formulated for embryonic neurons.

These two media have the same formulation and differ only in osmolality.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations & References (2)

Citations & References
Abstract
Survival and stimulation of neurite outgrowth in a serum-free culture of spiral ganglion neurons from adult mice.
Authors:Vieira M,Christensen BL,Wheeler BC,Feng AS,Kollmar R
Journal:Hearing research
PubMed ID:17521837
Patient-derived glioblastoma organoids as real-time avatars for assessing responses to clinical CAR-T cell therapy
Authors:Meghan Logun, Xin Wang, Yusha Sun, Stephen J Bagley, Nannan Li, Arati Desai, Daniel Y Zhang, MacLean P Nasrallah, Emily Ling-Lin Pai, Bike Su Oner, Gabriela Plesa, Donald Siegel, Zev A Binder, Guo-Li Ming, Hongjun Song, Donald M O'Rourke
Journal:Cell Stem Cell
PubMed ID:39657679
Patient-derived tumor organoids have been leveraged for disease modeling and preclinical studies but rarely applied in real time to aid with interpretation of patient treatment responses in clinics. We recently demonstrated early efficacy signals in a first-in-human, phase 1 study of dual-targeting chimeric antigen receptor (CAR)-T cells (EGFR-IL13Rα2 CAR-T cells) ... More