Search
Element Search
Structure Search
Thermo Scientific Chemicals
Trypan Blue, dye content >60%
CAS: 72-57-1 | C34H24N6Na4O14S4 | 960.79 g/mol
Have Questions?
Change view
Catalog number A18600.14
also known as A18600-14
Price (EUR)
36,20
Each
Quantity:
25 g
Price (EUR)
36,20
Each
Chemical Identifiers
CAS72-57-1
IUPAC Nametetrasodium (3Z)-5-amino-3-[2-(4'-{2-[(2Z)-8-amino-1-oxo-3,6-disulfonato-1,2-dihydronaphthalen-2-ylidene]hydrazin-1-yl}-3,3'-dimethyl-[1,1'-biphenyl]-4-yl)hydrazin-1-ylidene]-4-oxo-3,4-dihydronaphthalene-2,7-disulfonate
Molecular FormulaC34H24N6Na4O14S4
InChI KeyWMYVHJWZUUEZNE-ARWFNKCKSA-J
SMILES[Na+].[Na+].[Na+].[Na+].CC1=CC(=CC=C1N\N=C1\C(=O)C2=C(N)C=C(C=C2C=C1S([O-])(=O)=O)S([O-])(=O)=O)C1=CC=C(N\N=C2\C(=O)C3=C(N)C=C(C=C3C=C2S([O-])(=O)=O)S([O-])(=O)=O)C(C)=C1
View more
Specifications
Specification Sheet
Specification SheetAppearance (Color)Dark purple to dark brown or black
FormPowder
Assay (unspecified)≥60% dye content
Loss on Drying≤15.0% (200?C, constant weight)
lambda-max (UV-Vis)602-610 nm (methanol)
Trypan blue is commonly used in microscopy (for cell counting) and in laboratory mice for assessment of tissue viability. Used in dye exclusion studies in cell culture. A viability dye for quantifying live versus dead cells with permeable membranes, blue. It is also used to identify islet cell cluster cell viability from caprine pancreatic samples. It is also useful to observe hyphea of fungi and Stramenopiles. Trypan blue solution may be used in trypan blue based cytotoxitiy and proliferation assays.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Applications
Trypan blue is commonly used in microscopy (for cell counting) and in laboratory mice for assessment of tissue viability. Used in dye exclusion studies in cell culture. A viability dye for quantifying live versus dead cells with permeable membranes, blue. It is also used to identify islet cell cluster cell viability from caprine pancreatic samples. It is also useful to observe hyphea of fungi and Stramenopiles. Trypan blue solution may be used in trypan blue based cytotoxitiy and proliferation assays.
Solubility
Soluble in water (10 mg/ml at 25°C), ethanol (0.6 mg/ml), and EGME (20 mg/ml).
Notes
Store away from oxidizing agents. Keep the container tightly closed and place it in a cool, dry and well ventilated condition.
Trypan blue is commonly used in microscopy (for cell counting) and in laboratory mice for assessment of tissue viability. Used in dye exclusion studies in cell culture. A viability dye for quantifying live versus dead cells with permeable membranes, blue. It is also used to identify islet cell cluster cell viability from caprine pancreatic samples. It is also useful to observe hyphea of fungi and Stramenopiles. Trypan blue solution may be used in trypan blue based cytotoxitiy and proliferation assays.
Solubility
Soluble in water (10 mg/ml at 25°C), ethanol (0.6 mg/ml), and EGME (20 mg/ml).
Notes
Store away from oxidizing agents. Keep the container tightly closed and place it in a cool, dry and well ventilated condition.
RUO – Research Use Only
General References:
- McMahon TA.; Rohr JR. Trypan blue dye is an effective and inexpensive way to determine the viability of Batrachochytrium dendrobatidis zoospores.EcoHealth2014,11(2), 164-167.
- Serafini R.; Longobardi V.; Spadetta M.; Neri D.; Ariota B.; Gasparrini B.; Di Palo R. Trypan blue/giemsa staining to assess sperm membrane integrity in salernitano stallions and its relationship to pregnancy rates.Reproduction in Domestic Animals = Zuchthygiene,2014,49(1), 41-47.