TRA-1-60 Alexa Fluor™ 488 Conjugate Kit for Live Cell Imaging
TRA-1-60 Alexa Fluor™ 488 Conjugate Kit for Live Cell Imaging
Invitrogen™

TRA-1-60 Alexa Fluor™ 488 Conjugate Kit for Live Cell Imaging

Molecular Probes™ human pluripotent stem cell (PSC) live imaging kits provide reagents for superior imaging of live human PSCs. ThisRead more
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Catalog NumberQuantity
A2561850 tests
Catalog number A25618
Price (EUR)
480,00
Each
Add to cart
Quantity:
50 tests
Price (EUR)
480,00
Each
Add to cart
Molecular Probes™ human pluripotent stem cell (PSC) live imaging kits provide reagents for superior imaging of live human PSCs. This TRA-1-60 Alexa Fluor™ 488 Conjugate Kit includes an optimally labeled primary antibody paired with reduced-background imaging medium (FluoroBrite™ DMEM) for the specific detection of the PSC marker TRA-1-60. For fixed cell staining of PSC markers and germ layer markers, we recommend the Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit.

With the TRA-1-60 Alexa Fluor™ 488 Conjugate Kit for Live Cell Imaging, you can:
• Confirm the expression of a key pluripotent stem cell marker using an optimally dye-conjugated antibody
• Preserve cell health and maximize fluorescence signal with FluoroBrite™ DMEM
• Stain live cell samples with confidence using reagents that are tested to be free of common contaminants

Confirm Expression of a Key PSC Marker
Powered by the high performance of bright, photostable Alexa Fluor™ dyes, this kit enables reliable live-cell detection of the surface PSC marker TRA-1-60. This antibody conjugate has been demonstrated to show superior staining results when used with FluoroBrite™ DMEM.

Preserve Cell Health and Maximize Signal
Staining cells in FluoroBrite™ DMEM maximizes cell health and enhances signal-to-noise ratio when performing live-cell fluorescence imaging.

Stain Live-cell Samples with Confidence
Unlike most other conjugated antibodies, the reagents provided in this kit are 0.2 μm sterile-filtered and tested to be free of common contaminants.

What You Get
Easily scalable to any culture plate, dish, or slide format, the TRA-1-60 Alexa Fluor™ 488 Conjugate Kit comes with reagents sufficient to stain 50 samples using a 200 μL staining volume and includes:
• 200 μL of Alexa Fluor™ 488 Mouse Anti-Human TRA-1-60 (50X concentration)
• 500 mL FluoroBrite™ DMEM
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeConjugate Kit
Quantity50 tests
ConjugateAlexa Fluor 488
Product LineAlexa Fluor
Unit SizeEach
Contents & Storage
  • 200 μL of Alexa Fluor™ 488 Mouse Anti-Human TRA-1-60 (50 x concentration) (store at 2°C to 8°C)
  • 500 mL FluoroBrite™ DMEM
  • Store at 2°C to 8°C, protect from light
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Citations & References (2)

Citations & References
Abstract
Improved Generation of Induced Pluripotent Stem Cells From Hair Derived Keratinocytes - A Tool to Study Neurodevelopmental Disorders as ADHD.
Authors:Re S, Dogan AA, Ben-Shachar D, Berger G, Werling AM, Walitza S, Grünblatt E,
Journal:Front Cell Neurosci
PubMed ID:30319360
'In the last decade, there is an increasing application of induced pluripotent stem cells (iPSCs) for disease modeling. The iPSC technology enables the study of patient-specific neuronal cell lines in vitro to evaluate dysfunction at the cellular level and identify the responsible genetic factors. This approach might be particularly valuable ... More
Lineage-Specific Differentiation Is Influenced by State of Human Pluripotency.
Authors:Lee JH, Laronde S, Collins TJ, Shapovalova Z, Tanasijevic B, McNicol JD, Fiebig-Comyn A, Benoit YD, Lee JB, Mitchell RR, Bhatia M,
Journal:Cell Rep
PubMed ID:28380358
Human pluripotent stem cells (hPSCs) have been reported in naive and primed states. However, the ability to generate mature cell types remains the imperative property for utility of hPSCs. Here, we reveal that the naive state enhances self-renewal while restricting lineage differentiation in vitro to neural default fate. Molecular analyses indicate ... More