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Thermo Scientific™
EquiPhi29™ DNA Amplification Kit
The Thermo Scientific™ EquiPhi29 DNA Amplification Kit includes all required components for fast, sensitive, and efficient isothermal DNA amplification, which includes rolling circle amplification (RCA) and multiple displacement amplification – whole genome amplification (MDA-WGA).
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| Catalog Number | Quantity |
|---|---|
| A65394 | 500 Reactions |
| A65393 | 100 Reactions |
Catalog number A65394
Price (EUR)
1.698,00
Each
Quantity:
500 Reactions
Price (EUR)
1.698,00
Each
The Thermo Scientific™ EquiPhi29 DNA Amplification Kit includes all required components for fast, sensitive, and efficient isothermal DNA amplification, which includes rolling circle amplification (RCA) and multiple displacement amplification – whole genome amplification (MDA-WGA).
Features of the EquiPhi29 DNA Amplification Kit include:
- Fast DNA amplification in less than two hours
- Sensitivity from 1 fg of input DNA
- Maximal yield of 17 μg amplified DNA
- Compatibility with different types of sample input material (e.g., purified DNA, liquid media culture, agar plate colonies)
- Compatibility with various downstream applications such as DNA sequencing (Sanger sequencing, next-generation sequencing), digestion with restriction enzymes, cell-free DNA enrichment, and cell-free protein expression
- All required components for DNA amplification in RCA or MDA-WGA reactions
The kit contains EquPhi29 DNA Polymerase, a proprietary phi29 DNA polymerase mutant that possesses strong strand displacement activity and enables high thermostability, reaction speed, and product yield and low amplification bias. Also included is an optimized reaction buffer, exo-resistant random primer, pyrophosphatase, and dNTPs. The addition of pyrophosphatase to the reaction mixture enhances DNA amplification and facilitates sample handling.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatKit
Optimal Reaction Temperature42°C
Reaction TimeUp to 2 hours
Shipping ConditionDry Ice
For Use With (Application)RCA, MDA-WGA
FormLiquid
PolymeraseEquiPhi29 DNA Polymerase
Product TypeDNA Amplification Kit
Quantity500 Reactions
Research CategoryNucleic Acid Analysis, In Vitro Transcription, Cell-free Protein Expression, In Situ Sequencing, SNP Detection
Unit SizeEach
Contents & Storage
• 5 x 100 μL EquiPhi29 DNA Polymerase (10 U/μL)
• 2 x 1 mL EquiPhi29 DNA Polymerase Reaction Buffer (10X)
• 0.25 mL DTT (0.1 M)
• 5 x 200 μL Exo-Resistant Random Primer (500 μM)
• 1 mL dNTP Mix (10 mM each)
• 5 x 100 μL Pyrophosphatase, inorganic (0.1 U/μL)
Store at -15 to -25°C.