D-Biotin
D-Biotin
Citations & References (2)
Invitrogen™

D-Biotin

D-biotin binds with high affinity to streptavidin, avidin and NeutrAvidin™ biotin-binding proteins.Read more
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Catalog NumberQuantity
B2065610 mL
B15951 g
Catalog number B20656
Price (EUR)
183,00
Each
Add to cart
Quantity:
10 mL
Price (EUR)
183,00
Each
Add to cart
D-biotin binds with high affinity to streptavidin, avidin and NeutrAvidin™ biotin-binding proteins.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration50mM
Product TypeBiotin (unconjugated)
Quantity10 mL
Shipping ConditionRoom Temperature
ConjugateUnconjugated
FormLiquid
Product LineInvitrogen
Unit SizeEach
Contents & Storage
Store in refrigerator (2–8°C).

Frequently asked questions (FAQs)

How should I resolubilize D-biotin (B20656)?

D-biotin may be resolubilized in DMF (1-2 mg/mL), DMSO (49 mg/mL), ethanol (<1 mg/mL) or a buffer with a pH between >6 and <9. For long-term stability, D-biotin is stable in aqueous buffers at moderately acidic to neutral pH.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (2)

Citations & References
Abstract
Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation.
Authors:Hirsch JD, Eslamizar L, Filanoski BJ, Malekzadeh N, Haugland RP, Beechem JM, Haugland RP
Journal:Anal Biochem
PubMed ID:12419349
'The high-affinity binding of biotin to avidin, streptavidin, and related proteins has been exploited for decades. However, a disadvantage of the biotin/biotin-binding protein interaction is that it is essentially irreversible under physiological conditions. Desthiobiotin is a biotin analogue that binds less tightly to biotin-binding proteins and is easily displaced by ... More
A SPECTROPHOTOMETRIC ASSAY FOR AVIDIN AND BIOTIN BASED ON BINDING OF DYES BY AVIDIN.
Authors:GREEN NM,
Journal:Biochem J
PubMed ID:14340040
A spectrophotometric assay for avidin and biotin based on binding of dyes by avidin.