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6-JOE, SE (6-Carboxy-4',5'-Dichloro-2',7'-Dimethoxyfluorescein, Succinimidyl Ester)
Citations & References (15)
Invitrogen™

6-JOE, SE (6-Carboxy-4',5'-Dichloro-2',7'-Dimethoxyfluorescein, Succinimidyl Ester)

6-JOE, SE (6-Carboxy-4',5'-Dichloro-2',7'-Dimethoxyfluorescein, Succinimidyl Ester)
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Catalog NumberQuantity
C6171MP5 mg
Catalog number C6171MP
Price (EUR)
610,00
Each
Quantity:
5 mg
Price (EUR)
610,00
Each

This single isomer preparation of the amine-reactive dye 6-JOE, SE has fluorescence characteristics that are red-shifted as compared to fluorescein (excitation/emission is 522/550 nm) and is one of the traditional fluorophores used in automated DNA sequencing.

For Research Use Only. Not for use in diagnostic procedures.

Specifications
Chemical ReactivityAmine
Emission550
Excitation522
Label or DyeJOE
Product Type6-JOE SE
Quantity5 mg
Reactive MoietyActive Ester, Succinimidyl Ester
Shipping ConditionRoom Temperature
Label TypeClassic Dyes
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -30°C) and protect from light.

Citations & References (15)

Citations & References
Abstract
Ultra-high-speed DNA sequencing using capillary electrophoresis chips.
Authors:Woolley AT, Mathies RA
Journal:Anal Chem
PubMed ID:8644919
'DNA sequencing has been performed on microfabricated capillary electrophoresis chips. DNA separations were achieved in 50 x 8 microns cross-section channels microfabricated in a 2 in. x 3 in. glass sandwich structure using a denaturing 9% T, 0% C polyacrylamide sieving medium. DNA sequencing fragment ladders were produced and fluorescently ... More
Real-time PCR in the microbiology laboratory.
Authors:Mackay IM
Journal:Clin Microbiol Infect
PubMed ID:15008940
'Use of PCR in the field of molecular diagnostics has increased to the point where it is now accepted as the standard method for detecting nucleic acids from a number of sample and microbial types. However, conventional PCR was already an essential tool in the research laboratory. Real-time PCR has ... More
Energy-transfer fluorescent reagents for DNA analyses.
Authors:Glazer AN, Mathies RA
Journal:Curr Opin Biotechnol
PubMed ID:9013654
'Fluorescence resonance energy transfer has facilitated the development of a new class of high-performance fluorescent labeling reagents for multiplex analyses of nucleic acids. The enhanced emission of energy transfer (ET) primers has provided a decadic improvement in the performance of automated DNA sequencers. The emission spectral purity of ET primers ... More
Energy transfer primers with 5- or 6-carboxyrhodamine-6G as acceptor chromophores.
Authors:Hung SC, Ju J, Mathies RA, Glazer AN
Journal:Anal Biochem
PubMed ID:8660606
'Energy-transfer (ET) fluorescent primers for DNA sequencing and multiplex genetic analysis (Ju, J., Ruan, C., Fuller, C. W., Glazer, A. N., and Mathies, R. A. (1995) Proc. Natl. Acad. Sci. USA 92, 4347-4351) are named according to the convention D-N-A, where D is the donor, N is the number of ... More
Fluorescence excitation transfer immunoassay (FETI).
Authors:Ullman EF, Khanna PL
Journal:Methods Enzymol
PubMed ID:7033726
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